[1]马俊芬,李沛,黄幼田,等.曲古菌素A对食管癌细胞系EC1细胞凋亡的影响*[J].郑州大学学报(医学版),2010,(02):180-182.
 MA Junfen,LI Pei,HUANG Youtian,et al.Effects of trichostatin A on apoptosis in esophageal carcinoma cell line EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):180-182.
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曲古菌素A对食管癌细胞系EC1细胞凋亡的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年02期
页码:
180-182
栏目:
食管癌研究
出版日期:
2010-03-30

文章信息/Info

Title:
Effects of trichostatin A on apoptosis in esophageal carcinoma cell line EC1 cells
作者:
马俊芬李沛黄幼田杨洪艳郑智敏董子明#
郑州大学基础医学院病理生理学教研室郑州450001
Author(s):
MA JunfenLI PeiHUANG YoutianYANG HongyanZHENG ZhiminDONG Ziming
Department of Pathophysiology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
曲古菌素A细胞凋亡食管癌BaxBcl2EC1细胞
Keywords:
trichostatin Aapoptosisesophageal carcinomaBaxBcl2EC1 cell
分类号:
R735.1
文献标志码:
A
摘要:
探讨曲古菌素A(TSA)对食管癌EC1细胞凋亡的影响。方法:用DMSO(对照组)和0.3、0.5及1.0μmol/L的TSA处理EC1细胞24h,用AnnexinVFITC和PI双染色,流式细胞仪检测4组细胞的凋亡率,WesternBlot检测4组细胞中Bax及Bcl2表达的变化。结果:对照组、0.3、0.5及1.0μmol/LTSA组作用24h后,EC1细胞凋亡率分别为(2.18±0.37)%、(2.96±0.34)%、(7.41±1.01)%和(14.03±1.23)%,Bax蛋白表达水平分别为(0.42±0.23)、(0.45±0.11)、(1.16±0.28)和(1.89±0.30),Bcl2蛋白表达水平分别为(1.15±0.14)、(1.11±0.15)、(0.59±0.06)和(0.40±0.07),4组间3指标差异均有统计学意义(F=211.96,25.12和33.68,P均<0.001),随TSA作用浓度的增加,EC1细胞凋亡率、Bax蛋白表达均增加,Bcl2表达减少。结论:一定剂量的TSA可以诱导EC1细胞凋亡,凋亡的发生可能与Bax表达增加和Bcl2表达减少有关。
Abstract:
To explore the effects of trichostatin A on apoptosis in EC1 cells.Methods:EC1 cells at logarithmic growth phase were treated with 0.3 μmol/L, 0.5 μmol/L and 1.0 μmol/L TSA for 24 h, the cells were stained with Annexin VFITC and PI for analyzing apoptosis by flow cytometry, the expressions of Bax and Bcl2 were investigated with Western Blot.Cells treated with DMSO were the control.Results: Apoptosis rates of 0.3 μmol/L,0.5 μmol/L,1.0 μmol/L trichostatin A groups and the control group were (2.96±0.34)%,(7.41±1.01)%, (14.03±1.23)% and (2.18±0.37)%;the Bax expression level was (0.45±0.11),(1.16±0.28),(1.89±0.30) and (0.42±0.23);the Bcl2 expression level was (1.11±0.15),(0.59±0.06),(0.40±0.07), and (1.15±0.14). There were significant differences in the 3 indexes among the 4 groups(F=211.96,25.12,and 33.68,all P<0.001).Apoptosis rates and the Bax expression level increased and the Bcl2 expression level decreased with the increase of the dosage of TSA.Conclusion: TSA is able to induce apoptosis of esophageal carcinoma cell line EC1 cells partly through the regulation of the expression of Bax and Bcl2.

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备注/Memo

备注/Memo:
#通讯作者,男,1953年生,博士,教授,研究方向:肿瘤发病机制及生物治疗, Email:dongzm@zzu.edu.cn *教育部科技研究重大项目207150
更新日期/Last Update: 2010-04-29