[1]何卫华,史惠蓉#.卵巢上皮性癌组织中黏着斑激酶和肝癌缺失基因1 mRNA的表达*[J].郑州大学学报(医学版),2010,(02):209-212.
 HE Weihua,SHI Huirong.Expression of focal adhesion kinase and deleted in liver cancer 1 mRNA in epithelial ovarian cancer tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):209-212.
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卵巢上皮性癌组织中黏着斑激酶和肝癌缺失基因1 mRNA的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年02期
页码:
209-212
栏目:
论著
出版日期:
2010-03-30

文章信息/Info

Title:
Expression of focal adhesion kinase and deleted in liver cancer 1 mRNA in epithelial ovarian cancer tissue
作者:
何卫华史惠蓉#
郑州大学第一附属医院妇产科郑州450052
Author(s):
HE WeihuaSHI Huirong
Department of Gynecology and Obstetrics,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
黏着斑激酶肝癌缺失基因1卵巢上皮性肿瘤
Keywords:
focal adhesion kinasedeleted in liver cancer 1epithelial ovarian neoplasmcancer
分类号:
R737.31
文献标志码:
A
摘要:
检测卵巢上皮性癌组织中黏着斑激酶(FAK)和肝癌缺失基因1(DLC1)mRNA的表达。方法:采用逆转录聚合酶链反应检测12例正常卵巢和32例卵巢上皮性癌组织中FAK与DLC1mRNA的表达。结果:卵巢上皮性癌组织中FAKmRNA的表达水平(0.81±0.33)高于正常卵巢组织(0.24±0.15),2者相比,差异有统计学意义(t=2.979,P=0.007);FAKmRNA的表达水平与卵巢上皮性癌的分化程度、淋巴结转移和腹水形成有关(t/F=3.405,2.223和2.330,P均<0.05)。卵巢上皮性癌组织中DLC1mRNA的表达水平(0.30±0.11)低于正常卵巢组织(0.68±0.17),2者相比,差异有统计学意义(t=-3.284,P=0.013);DLC1mRNA的表达与卵巢上皮性癌的临床分期、淋巴结转移和腹水形成有关(t/F=3.156,2.944和2.470,P均<0.05)。卵巢上皮性癌组织中FAK与DLC1mRNA的表达有关联(rP=0.369,P=0.025)。结论:FAK的过表达和DLC1低表达或表达缺失可能与卵巢上皮性癌的发生、发展有关;联合检测2者有助于判断卵巢上皮性癌的恶性程度。
Abstract:
To detect the expression of FAK mRNA and DLC1 mRNA in epithelial ovarian cancer tissue.Methods:RTPCR was used to detect the expression of FAK mRNA and DLC1 mRNA in epithelial ovarian cancer and normal ovarian tissue.Results: The expression level of FAK mRNA in epithelial ovarian cancer tissue(0.81±0.33)was significantly higher than that in normal ovarian tissue[(0.24±0.15),t=2.979,P=0.007];the expression level of FAK mRNA was related with differentiation,lymphatic metastasis and ascites formation(t/F=3.405,2.223 and 2.330,all P<0.05). The expression level of DLC1 mRNA in epithelial ovarian cancer tissue(0.30±0.11) was significantly lower than that in normal ovarian tissue[(0.68±0.17),t=-3.284,P=0.013].The expression level of DLC1 mRNA was related with clinical stage,ascites formation and lymphatic metastasis(t/F=3.156,2.944 and 2.470,all P<0.05).The expression of FAK mRNA had association with that of DLC1 mRNA(rP=0.369,P=0.025).Conclusion: The overexpression of FAK and the underexpression or loss of DLC1 may play important roles in the generation and development of epithelial ovarian cancer;combined detection of FAK and DLC1 may be helpful in predicting the maligant degree of epithelial ovarian cancer.

参考文献/References:

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[2]Liao YC, Lo SH. Deleted in liver cancer1 (DLC1): a tumor suppressor not just for liver[J]. Int J Biochem Cell Biol, 2008,40(5):843
[3]Kim TY,Lee JW,Kim HP,et al.DLC1,a GTPaseactivating protein for Rho, is associated with cell proliferation,morphology,and migration in human hepatocellular carcinoma[J]. Biochem Biophys Res Commun,2007, 355(1):72
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备注/Memo

备注/Memo:
*河南省医学科技攻关计划基金资助项目20090113#通讯作者,女,1956年生,博士,教授,研究方向:妇科肿瘤,Email:huirongshi@yahoo.com.cn
更新日期/Last Update: 2010-04-29