[1]张勇,娄卫华#,李爱霞.罗格列酮对Hep2细胞核因子κB和血管内皮生长因子mRNA表达的影响[J].郑州大学学报(医学版),2010,(02):262-265.
 ZHANG Yong,LOU Weihua,LI Aixia.Effects of rosiglitazone on mRNA expressions of nuclear factorκB and vascular endothelial growth factor in Hep2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):262-265.
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罗格列酮对Hep2细胞核因子κB和血管内皮生长因子mRNA表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年02期
页码:
262-265
栏目:
论著
出版日期:
2010-03-30

文章信息/Info

Title:
Effects of rosiglitazone on mRNA expressions of nuclear factorκB and vascular endothelial growth factor in Hep2 cells
作者:
张勇娄卫华#李爱霞
郑州大学第一附属医院耳鼻喉科郑州450052
Author(s):
ZHANG YongLOU WeihuaLI Aixia
Department of Otorhinolaryngology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
喉癌罗格列酮Hep2细胞核因子κB血管内皮生长因子
Keywords:
laryngeal carcinoma rosiglitazone Hep2 cell nuclear factorκB vascular endothelial growth factor
分类号:
R739.65
文献标志码:
A
摘要:
通过检测罗格列酮(ROS)作用前后人喉癌Hep2细胞核因子(NFκB)和血管内皮生长因子(VEGF)mRNA表达的变化情况,探讨其抑制Hep2增殖的机制。方法:采用MTT法检测12.5、25.0、50.0和100.0μmol/LROS处理12、24、36、48、60和72h后Hep2细胞的增殖抑制率。RTPCR方法检测50μmol/L的ROS处理0、24、48和72h后Hep2细胞NFκB和VEGFmRNA表达的变化。结果:ROS对Hep2细胞的生长具有增殖抑制作用,其作用表现为剂量依赖性(12、24、36、48、60和72h各浓度组相比,F为117.584、98.241、92.352、117.228、148.458和124.900,P均<0.001)和时间依赖性(12.5、25.0、50.0和100.0μmol/L各时间组相比,F为140.949、123.335、148.325和176.590,P均<0.001)。RTPCR检测结果显示50μmol/L的ROS处理Hep2细胞0、24、48和72h后,NFκB相对表达量分别为(0.854±0.066)、(0.653±0.059)、(0.489±0.027)和(0.336±0.031),差异有统计学意义(F=111.357,P<0.001);VEGF相对表达量分别为(0.756±0.057)、(0.628±0.039)、(0.482±0.044)和(0.318±0.035),差异有统计学意义(F=89.796,P<0.001)。结论:ROS具有抑制Hep2细胞增殖的作用,其机制与下调NFκB和VEGFmRNA表达有关。
Abstract:
By detecting nuclear factorκB(NFκB)and vascular endothelial growth factor(VEGF) expression changes in human laryngeal carcinoma Hep2 cells before and after being treated with rosiglitazone(ROS), to explore its role in Hep2 proliferation inhibition.Methods:MTT method was used to observe the proliferation of Hep2 cells treated with 12.5,25.0,50.0, and 100.0 μmol/L ROS for 12,24,36,48,60,and 72 hours. RTPCR method was used to detect the expressions of NFκB and VEGF mRNA in the Hep2 cells treated with ROS at 50 μmol/L for 0,24,48,and 72 hours.Results: ROS inhibited growth of Hep2 cells in a dosedependent (at 12, 24, 36, 48, 60, and 72 h each concentration group,F were 117.584, 98.241, 92.352, 117.228, 148.458,and 124.900, P<0.001) and timedependent manner (12.5, 25.0,50.0, and 100.0 μmol/L groups at different time point,F were 140.949, 123.335, 148.325,and 176.590, P<0.001).The expression of NFκB mRNA was (0.854±0.066), (0.653±0.059), (0.489±0.027), and (0.336±0.031) at 0, 24, 48, and 72 h after the treatment by ROS at 50 μmol/L,and there was significant difference among different time groups(F=111.357,P<0.001).The expression of VEGF mRNA was (0.756±0.057), (0.628±0.039), (0.482±0.044), and (0.318±0.035) at 0, 24, 48, and 72 h,and there was significant difference among different time groups(F=89.796,P<0.001).Conclusion: ROS could inhibit Hep2 cell proliferation,and its mechanism is related to downregulating NFκB and VEGF mRNA expression.

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备注/Memo

备注/Memo:
#通讯作者,男,1959年生,博士,教授,研究方向:头颈肿瘤防治
更新日期/Last Update: 2010-04-29