[1]崔广晖),齐宇)#,赵松),等.FTY720对大鼠同种异体异位心脏移植物缺血再灌注损伤的影响[J].郑州大学学报(医学版),2010,(02):272-274.
 CUI Guanghui),QI Yu),ZHAO Song),et al.Effects of FTY720 on ischemiareperfusion injury of rat cardiac allografts[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(02):272-274.
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FTY720对大鼠同种异体异位心脏移植物缺血再灌注损伤的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年02期
页码:
272-274
栏目:
论著
出版日期:
2010-03-30

文章信息/Info

Title:
Effects of FTY720 on ischemiareperfusion injury of rat cardiac allografts
作者:
崔广晖1)齐宇1)#赵松1)廖崇先2)马启明3)王具明3)
1)郑州大学第一附属医院胸外科郑州4500522)厦门大学附属中山医院心脏中心厦门3610043)杭州同明医化科技开发公司杭州310011
Author(s):
CUI Guanghui1)QI Yu1)ZHAO Song1) LIAO Chongxian2) MA Qiming3) WANG Juming3)
1)Department of Thoracic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Heart Center,Zhongshan Hospital, Xiamen University, Xiamen 3610043)Hangzhou Tongming Medical Chemistry Science & Technology Development Co.,Ltd.,Hangzhou 310011
关键词:
FTY720缺血再灌注损伤心脏移植大鼠
Keywords:
FTY720ischemiareperfusion injuryheart transplantationrat
分类号:
R654.2
文献标志码:
A
摘要:
观察FTY720对同种异体异位心脏移植物缺血再灌注损伤的影响并探讨其相关机制。方法:利用Cuff技术建立颈部异位心脏移植模型。FTY720组(n=6)受体鼠自术前3d开始至手术当日管饲FTY720,剂量为3mg/kg;对照组(n=6)受体鼠自术前3d开始至手术当日管饲生理盐水,剂量为1mL/kg。于移植后8h切取移植心脏,电镜下观察心肌组织超微结构变化;TUNEL法测细胞凋亡,计算凋亡指数;测定心肌组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量和髓过氧化物酶(MPO)活性。结果:移植后8h,FTY720组心肌细胞形态基本正常,仅轻度水肿,间质水肿和炎性细胞浸润不明显;电镜超微结构显示心肌肌丝排列整齐,肌原纤维间轻度水肿,线粒体饱满,嵴密,水肿不明显,无明显的肌丝断裂现象。FTY720组只有少量心肌细胞凋亡,凋亡指数为(9.20±1.12),对照组心肌细胞凋亡明显,凋亡指数为(16.10±1.65)(t=8.475,P<0.001)。FTY720组SOD活性较对照组升高[(51.03±5.54)mmol/gvs.(28.39±2.38)mmol/g],而MDA含量[(10.90±1.93)U/gvs.(16.27±1.36)U/g]和MPO活性[(4.38±1.43)mU/gvs.(9.26±1.41)mU/g]下降(t分别为9.197、5.571和5.592,P均<0.001)。结论:FTY720能减轻大鼠移植心脏缺血再灌注损伤。
Abstract:
To study the effects of FTY720 on ischemiareperfusion injury of rat cardiac allografts.Methods:The model of rat heterotopic heart transplantation was established with Cuff technique. The recipient rats of the FTY720treated group (n=6) was administered with FTY720 orally at a dosage of 3 mg/kg for 3 days before grafting operation,and the control group was administered with normal saline at a dosage of 3 mL/kg. All recipients were sacrificed and the donor hearts were removed at 8 hours after transplantation. The ultrastructure of the graft tissues was observed under electron microscope. Apoptosis index (AI) in grafts was determined by TUNEL. Grafts’ SOD activity, MDA content, and MPO activity were measured.Results: At 8 hours after transplantation in FTY720treated group, there were less cellular swelling, less inflammatory cell infiltration and less mitochondrial oedema of cadiocyte.Compared with control group,the cell apoptosis in FTY720treated group decreased [AI:(9.20±1.12) vs. (16.10±1.65),t=8.475,P<0.001)],SOD activity [(51.03±5.54) mmol/g vs. (28.39±2.38) mmol/g] was higher,and MDA content[(10.90±1.93) U/g vs. (16.27±1.36) U/g] and MPO activity [(4.38±1.43) mU/g vs. (9.26±1.41) mU/g] were lower(t=9.197,5.571, and 5.592,P<0.001).Conclusion:FTY720 could ameliorate the myocardial ischemiareperfusion injury in rat heart transplantation.

参考文献/References:

[1]Hauptman PJ, Aranki S, Mudge GH Jr, et al. Early cardiac allograft failure after orthotopic heart transplantation[J]. Am Heart J, 1994,127(1):179
[2]孙博, 彭志海, 刘皋林. 免疫抑制剂在器官移植中的应用及研究进展[J]. 中国药学杂志, 2008,43(20):1 530
[3]殷亮, 李兵, 殷海泉. 新型免疫抑制剂FTY720在器官移植及眼科中的应用[J]. 中国组织工程与临床康复, 2009, 13(18):3 483
[4]崔广晖, 赵松, 王铁栓, 等. 大鼠同种异体颈部心脏移植动物模型的建立[J]. 医药论坛杂志, 2009, 30(3):40
[5]Krown KA, Page MT, Ngayen C. Tumor necrosis factorαinduced apoptosis in cardiac myocytes[J]. J Clin Invest,1996,98(12):2 854
[6]Gottlieb RA, Burleson CO, Kloner RA, et al. Reperfusion injury induces apoptosis in rabbit cardiomyocytes[J]. J Clin Invest, 1994, 94(4):1 621
[7]Ushitora Y,Tashiro H,Ogawa T,et al.Suppression of hepatocellular carcinoma recurrence after rat liver transplantation by FTY720,a sphingosine1phosphate analog[J].Transplantation,2009,88(8):980
[8]孙飞, 徐兴伟.免疫抑制剂在脊髓损伤中应用的研究现状[J].医学综述, 2009, 15(21):3 253
[9]黄洪锋,陈江华.新型免疫调节剂FTY720研究进展[J].国外医学:泌尿系统分册,2004,24(1):117
[10]Troncoso P, Ortiz M, Martinez L, et al. FTY 720 prevents ischemic reperfusion damage in rat kidneys[J].Transplant Proc, 2001, 33(1/2):857

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备注/Memo

备注/Memo:
#通讯作者,男,1978年生,博士研究生,主治医师,研究方向:心肺移植,Email:ekansqy@gmail.com
更新日期/Last Update: 2010-04-29