[1]王琼叶),侯桂琴),王莉莉),等.雷帕霉素抑制mTOR信号通路对EC9706细胞生长及凋亡的影响*[J].郑州大学学报(医学版),2010,(03):356-358.
 WANG Qiongye),HOU Guiqin),WANG Lili),et al.Effects of rapamycin inhibiting mTOR signaling pathway on growth and apoptosis of esophageal squamous cell carcinoma cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):356-358.
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雷帕霉素抑制mTOR信号通路对EC9706细胞生长及凋亡的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年03期
页码:
356-358
栏目:
食管癌研究
出版日期:
2010-05-15

文章信息/Info

Title:
Effects of rapamycin inhibiting mTOR signaling pathway on growth and apoptosis of esophageal squamous cell carcinoma cells
作者:
王琼叶1)侯桂琴2)王莉莉1)薛乐勋13)#
1)郑州大学第一附属医院肿瘤细胞生物研究室郑州4500522)郑州大学药学院药理学教研室郑州4500013)郑州大学生物工程系细胞生物研究室郑州450001
Author(s):
WANG Qiongye1)HOU Guiqin2)WANG Lili1)XUE Lexun13)
关键词:
食管鳞状细胞癌哺乳动物雷帕霉素靶蛋白雷帕霉素EC9706
Keywords:
esophageal squamous cell carcinomamammalian target of rapamycinrapamycinEC9706 cells
分类号:
R735.1
摘要:
检测食管鳞状细胞癌组织中哺乳动物雷帕霉素靶蛋白(mTOR)的活性及雷帕霉素(rapa)对食管鳞状细胞癌细胞系EC9706细胞生长及凋亡的影响。方法:采用免疫组织化学SP法检测50例食管鳞状细胞癌组织及15例正常食管组织中mTOR蛋白的表达。分别用不同浓度(20、50和100 nmol/L )的rapa处理食管鳞状细胞癌EC9706细胞系,以未处理组作为阴性对照,采用流式细胞术测定细胞周期和凋亡率。结果:食管鳞状细胞癌组织中mTOR蛋白的阳性表达率为64.0%(32/50),高于正常组织的13.3%(2/15)(χ2=11.873, P=0.001)。与对照组比较,rapa可使细胞停滞于G0/G1期(F=102.609,P=0.001),且明显促进细胞凋亡(F=916.882,P=0.001)。结论:mTOR信号通路在食管鳞状细胞癌中被显著激活,rapa能抑制食管鳞状细胞癌细胞生长并诱导细胞凋亡。
Abstract:
To study the activation of mammalian target of rapamycin(mTOR) signaling pathway in esophageal squamous cell carcinoma (ESCC) and the effects of rapamycin on the cell growth and apoptosis of ESCC cell line EC9706.Methods:The expressions of mTOR in 50 ESCC tissues and 15 normal esophageal tissues were detected by immunohistochemistry. The cell cycle and cell apoptosis were determined by using flow cytometry in EC9706 cells treated with rapamycin at different concentrations (20, 50 and 100 nmol/L), and the untreated cells were used as control.Results: The expression rate of mTOR in ESCC tissues was 64.0%(32/50),higher than that in normal esophageal tissues,and there was obvious difference(χ2=11.873,P=0.001).

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1942年2月生,教授,研究方向:肿瘤与生物工程,Email:xuelx@371.net*国家自然科学基金资助项目30901778;教育部“十·五”“211工程”重点学科建设项目教重字20022
更新日期/Last Update: 2010-05-28