[1]王琳),徐春燕),刘新奎),等.热化疗对H446细胞增殖的影响*[J].郑州大学学报(医学版),2010,(03):375-377.
 WANG Lin),XU Chunyan),LIU Xinkui),et al.Effect of thermochemotherapy on proliferation of H446 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):375-377.
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热化疗对H446细胞增殖的影响*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年03期
页码:
375-377
栏目:
论著
出版日期:
2010-05-15

文章信息/Info

Title:
Effect of thermochemotherapy on proliferation of H446 cells
作者:
王琳1)徐春燕2)刘新奎3)师国珍1)#
1)郑州大学第一附属医院放疗科;河南省高等学校临床医学重点学科开放实验室郑州4500522)郑州大学学报编辑部郑州4500013)郑州大学第一附属医院病案管理科;河南省高等学校临床医学重点学科开放实验室郑州450052
Author(s):
WANG Lin1)XU Chunyan2) LIU Xinkui3)SHI Guozhen1)
关键词:
H446细胞化疗热化疗Akt细胞增殖
Keywords:
H446 cell chemotherapythermochemotherapyAktcell proliferation
分类号:
R730.58
摘要:
研究热化疗对人小细胞肺癌H446细胞增殖的影响及其可能机制。方法:H446细胞分为单纯化疗组(单纯使用120 μg/L紫杉醇处理细胞)、热化联合组(采用43 ℃加热联合120 μg/L紫杉醇处理细胞)、抑制剂组(采用43 ℃加热联合120 μg/L紫杉醇及1 μmol/L Akt特异抑制剂wortmannin处理细胞),以未处理的H446细胞作对照。应用MTT法检测各组细胞增殖率的变化,采用Western Blot检测各组细胞磷酸化Akt水平。结果:对照组、单纯化疗组、热化联合组和抑制剂组细胞增殖率分别为(100.00±0.00)%、(69.16±2.95)%,(59.83±3.36)%和(40.65±0.14)%,磷酸化AKt水平分别为(1.52±0.01),(1.04±0.42),(0.69±0.03)和(0.00±0.00),4组细胞增殖率及磷酸化Akt水平相比,差异均有统计学意义(F=68.231和6.232,P均<0.05)。与对照组和单纯化疗组相比,热化联合组细胞增殖率及磷酸化Akt水平均降低(P<0.05),wortmannin可进一步抑制H446细胞的磷酸化,且降低细胞的增殖率(P<0.05)。结论:热化疗联合应用可抑制H446细胞增殖,其作用可能是通过抑制Akt信号转导通路实现的。
Abstract:
To study the effect of thermochemotherapy on the proliferation of lung cancer H446 cells and its possible mechanism.Methods:H446 cells were allocated into 4 groups:cells in chemotherapy group were given 120 μg/L paclitaxel,those in thermochemotherapy group were treated by paclitaxel and heating at 43 ℃,those in inhibitor group were treated by Akt inhibitor,wortmannin, besides the treatment as thermochemotherapy group.The cells untreated served as control. MTT assay was used to measure cell proliferation,and Western Blot was used to examine the phosphorylation level of Akt.

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备注/Memo

备注/Memo:
#通讯作者,男,1947年10月生,主任医师,教授,研究方向:细胞药物时相与放射线细胞时相的结合治疗恶性肿瘤,Email:shiguozhen0371@126.com *国家自然科学基金资助项目30571552;郑州市科技发展计划(攻关计划)基金资助项目19153(2006)
更新日期/Last Update: 2010-05-28