[1]陈广理)△,龚树生),陈沛),等.Hep2细胞中bFGF及其双受体系统mRNA的表达*[J].郑州大学学报(医学版),2010,(03):404-406.
 CHEN Guangli),GONG Shusheng),CHEN Pei),et al.Expressions of bFGF,perlecan and FGFR mRNA in Hep2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):404-406.
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Hep2细胞中bFGF及其双受体系统mRNA的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年03期
页码:
404-406
栏目:
论著
出版日期:
2010-05-15

文章信息/Info

Title:
Expressions of bFGF,perlecan and FGFR mRNA in Hep2 cells
作者:
陈广理12)龚树生3)陈沛1)罗凌惠1)
1)华中科技大学同济医学院附属协和医院耳鼻咽喉科武汉4300222)洛阳东方医院耳鼻咽喉科洛阳4710033)首都医科大学附属北京同仁医院耳鼻咽喉头颈外科北京100730
Author(s):
CHEN Guangli12)GONG Shusheng3)CHEN Pei1) LUO Linghui1)
关键词:
Hep2细胞碱性成纤维细胞生长因子串珠素高亲和力酪氨酸激酶受体喉癌
Keywords:
Hep2 cellbasic fibroblast growth factorperlecanhighaffinity fibroblast growth factor receptorlaryngeal carcinoma
分类号:
R739.65
摘要:
研究碱性成纤维细胞生长因子(bFGF)及其双受体系统串珠素(perlecan)和高亲和力酪氨酸激酶受体(FGFR)在人喉癌细胞Hep2中的表达。方法:培养Hep2和正常永生化表皮细胞系HaCaT细胞,应用RTPCR方法检测bFGF、perlecan及FGFR1~4 mRNA的表达。结果:bFGF、 perlecan、FGFR1、FGFR2及FGFR4 mRNA在 Hep2细胞中的相对表达量高于HaCaT细胞(t分别为 17.540、19.684、13.371、12.692和12.586,P均<0.05)。2种细胞中均未检测到FGFR3 mRNA。结论:bFGF、perlecan及FGFR与喉癌细胞的生长关系密切。
Abstract:
To study the expressions of basic fibroblast growth factor (bFGF),perlecan and the four highaffinity FGF receptors(FGFR) in human laryngeal carcinoma cells.Methods:The expressions of bFGF,perlecan and FGFR1~4 mRNA in human laryngeal carcinoma cells Hep2 were detected using techniques of semiquantity RTPCR.

参考文献/References:

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备注/Memo

备注/Memo:
△男,1971年4月生,博士,副主任医师,研究方向:耳鼻咽喉疾病的基础与临床,Email:glchen71@yahoo.com.cn*第4届教育部“高校青年教师奖”基金资助项目
更新日期/Last Update: 2010-05-28