[1]张军辉,赵玉林#,张勇.丙戊酸钠对Hep2细胞增殖及COX2 和Survivin mRNA表达的影响[J].郑州大学学报(医学版),2010,(03):440-442.
 ZHANG Junhui,ZHAO Yulin,ZHANG Yong.Effects of sodium valproate on proliferation and expressions of COX2 and Survivin mRNA in Hep2 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):440-442.
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丙戊酸钠对Hep2细胞增殖及COX2 和Survivin mRNA表达的影响()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年03期
页码:
440-442
栏目:
论著
出版日期:
2010-05-15

文章信息/Info

Title:
Effects of sodium valproate on proliferation and expressions of COX2 and Survivin mRNA in Hep2 cells
作者:
张军辉赵玉林#张勇
郑州大学第一附属医院耳鼻喉科郑州450052
Author(s):
ZHANG JunhuiZHAO YulinZHANG Yong
Department of Otorhinolaryngology, the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
喉癌丙戊酸钠Hep2细胞增殖环氧化酶2Survivin
Keywords:
laryngeal carcinomasodium valproateHep2 cellproliferationCOX2 Survivin
分类号:
R739.65
摘要:
探讨丙戊酸钠(VPA)对体外培养的人喉癌Hep2细胞增殖及环氧化酶2(COX2)和Survivin mRNA表达的影响。方法:以1、2、3、4和5 mmol/L的VPA处理人喉癌Hep2细胞12、24、36、48、60和72 h后,采用MTT法检测细胞的增殖活性,RTPCR方法检测3 mmoL/L VPA处理Hep2细胞0、24、48和72 h后COX2和Survivin mRNA的表达。结果:VPA对人喉癌Hep2细胞的生长具有明显的抑制作用,其作用表现为剂量依赖性(F分别为50.518,80.602,164.699,236.579和243.972,P均<0.001)和时间依赖性(F分别为63.067,126.190,197.807,254.084,294.646和334.773,P均<0.001)。以3 mmoL/L的VPA处理人喉癌Hep2细胞,随作用时间的延长,COX2 和Survivin mRNA的表达逐渐降低(F分别为210.290和76.773,P均<0.001)。结论:VPA对Hep2细胞具有明显的增殖抑制作用,其作用机制与下调COX2和Survivin mRNA的表达有关。
Abstract:
To observe the effects of sodium valproate(VPA) on proliferation and expressions of COX2 and Survivin mRNA in human laryngeal carcinoma Hep2 cells.Methods:MTT was used to observe the proliferation of Hep2 cells treated with various concentrations (1,2,3,4,and 5 mmol/L) of VPA for different time(12,24,36,48,60,and 72 h).

参考文献/References:

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备注/Memo

备注/Memo:
#通讯作者,男,1964年10月生,教授,主任医师,研究方向:头颈部肿瘤的防治
更新日期/Last Update: 2010-05-28