[1]孙广莉△,张明昌,蒋丽.粉防己碱对人翼状胬肉成纤维细胞体外增殖及增殖细胞核抗原表达的影响[J].郑州大学学报(医学版),2010,(03):451-452.
 SUN Guangli,ZHANG Mingchang,JIANG Li.Effects of tetrandrine on proliferation and expression of PCNA of human pterygium fibroblasts[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(03):451-452.
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粉防己碱对人翼状胬肉成纤维细胞体外增殖及增殖细胞核抗原表达的影响 ()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年03期
页码:
451-452
栏目:
论著
出版日期:
2010-05-15

文章信息/Info

Title:
Effects of tetrandrine on proliferation and expression of PCNA of human pterygium fibroblasts
作者:
孙广莉张明昌蒋丽
郑州大学第一附属医院眼科郑州450052
Author(s):
SUN GuangliZHANG MingchangJIANG Li
Department of Ophthalmology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
粉防己碱翼状胬肉成纤维细胞增殖增殖细胞核抗原
Keywords:
tetrandrinepterygiumfibroblastprolifertionproliferating cell nuclear antigen
分类号:
R777.33
摘要:
观察粉防己碱(Tet)对体外培养人翼状胬肉成纤维细胞(HPF)增殖的影响。方法:用20、40、80和160 μmol/L的Tet作用于体外培养的HPF,24、48及72 h后采用MTT法检测细胞生长抑制率, 免疫细胞化学法检测HPF增殖细胞核抗原(PCNA)的表达情况。结果:Tet在20、40、80和160 μmol/L浓度下作用24、48及72 h,能够抑制HPF增殖(F时间=235.632、342.039、586.788和584.667,P均<0.001;F组间=372.672、474.734和389.467,P均<0.001)。Tet干预后PCNA蛋白表达下降(F=185.623,P=0.009)。结论:Tet可抑制翼状胬肉HPF细胞增殖。
Abstract:
To investigate the effects of tetrandrine on proliferation of human pterygium fibroblast (HPF) in vitro.Methods:Different concentrations (20,40,80, and 160 μmol/L) of Tet were added into cultured HPF for 24,48, and 72 hours.

参考文献/References:

[1]Coroneo MT, Di Girolamo N, Wakefield D. The pathogenes is of pterygia[J].Curr Opin Ophthalmol,1999,10(4):282
[2]Tan DT,Chee SP, Dear KB,et al.Effect of pterygium morphology on pterygium recurrence in a controlled trial comparing conjunctival autografting with bare sclear excision[J].Arch Ophthalmol,1997,115(10):1 235
[3]徐永红,边城,李定国,等.汉防己甲素对成纤维细胞增殖及胶原合成的作用[J].天津医药,2004,32(10):633
[4]刘德伍,李国辉,刘德明,等.粉防己碱抑制瘢痕形成机理的研究[J].中药药理与临床,2000,16(6):10
[5]刘德伍,李国辉,邹萍,等.粉防己碱对PDGF、TGF2β诱导瘢痕成纤维细胞增殖与胶原合成的抑制作用[J].中药药理与临床, 2004,20(4):10
[6]Ohta Y, Ichimura K. Proliferation markers, proliferating cell nuclear antigen, Ki67, 5bromo2’deoxyuridine, and cyclin D1 in mouse olfactory epithelium[J].Ann Otol Rhinol Laryngol, 2000,109(11):1 046

相似文献/References:

[1]段昱△,吕品,贺付成,等.粉防己碱对EC9706细胞增殖、细胞周期及sPLA2Ⅱa蛋白表达的影响[J].郑州大学学报(医学版),2013,(03):303.
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备注/Memo

备注/Memo:
△女,1977年5月生,博士,主治医师,研究方向:眼表疾病,Email:sunguangli1@126.com
更新日期/Last Update: 2010-05-28