[1]彭涛,滕军放,关文娟,等.盐酸法舒地尔体外诱导大鼠骨髓间充质干细胞向神经元样细胞分化的可行性[J].郑州大学学报(医学版),2010,(04):559.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):559.
点击复制

盐酸法舒地尔体外诱导大鼠骨髓间充质干细胞向神经元样细胞分化的可行性()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年04期
页码:
559
栏目:
论著
出版日期:
2010-07-30

文章信息/Info

作者:
彭涛滕军放关文娟文全庆张博爱贾延劼
郑州大学第一附属医院神经内科 郑州 450052
关键词:
盐酸法舒地尔骨髓间充质干细胞分化神经元大鼠
文献标志码:
A
摘要:
目的:探讨Rho/Rho激酶(ROCK)抑制剂盐酸法舒地尔体外诱导大鼠骨髓间充质干细胞(MSCs)向神经元样细胞分化的可行性。方法:全骨髓培养法分离培养大鼠骨髓MSCs,用200 μmol/L盐酸法舒地尔诱导分化。倒置显微镜观察诱导不同时间细胞的形态学变化;AOEB染色法鉴定诱导后细胞存活率;免疫荧光法鉴定诱导后神经元特异性烯醇化酶(NSE)、神经丝蛋白(NF200)与胶质纤维酸性蛋白(GFAP)的表达,判断其分化情况。结果:盐酸法舒地尔诱导30、60、90、120及180 min后细胞存活率分别为(96.7±2.2)%、(95.3±1.9)%、(93.8±1.8)%、(92.5±2.1)%及(90.1±1.3)%,其中以盐酸法舒地尔诱导120 min后,形态变化最为理想。盐酸法舒地尔诱导60、90、120及180 min后, NSE阳性表达率分别为(66.5±1.9)%、(88.1±3.2)%、(93.6±1.9)%和(93.5±5.4)%,NF200阳性表达率分别为(70.1±2.9)%、(89.5±1.3)%、(98.1±1.6)%和(98.3±1.9)%,而GFAP阳性表达率均小于5%。结论:盐酸法舒地尔可在体外快速、高效地诱导大鼠MSCs向神经元样细胞分化。

参考文献/References:

[1]Woodbury D,Schwarz EJ,Prockop DJ,et al. Adult rat and human bone marrow stromal cells differentiate into neurons[J].J Neurosci Res,2000,61(4):364
[2]EtienneManneville S, Hall A.Rho GTPases in cell biology[J].Nature,2002,420(6 916):629
[3]Riento K,Ridley AJ. Rocks:multifunctional kinases in cell behaviour[J].Nat Rev Mol Cell Biol,2003,4(6):446
[4]Yaghoobi MM,Mowla SJ,Tiraihi T. Nucleostemin,a coordinator of selfrenewal, is expressed in rat marrow stromal cells and turns off after induction of neural differentiation[J].Neurosci Lett,2005,390(2):81
[5]WisletGendebien S,Hans G,Leprince P,et al. Plasticity of cultured mesenchymal stem cells:switch from nestinpositive to excitable neuronlike phenotype[J].Stem Cells,2005,23(3):392
[6]Brown JH,Del Re  DP,Sussman MA. The Rac and Rho  hall of fame: a decade of hypertrophic signaling hits[J].Circ Res,2006,98(6):730
[7]Pacary E,Legros H,Valable S,et al. Synergistic effects of CoCl(2) and ROCK inhibition on mesenchymal stem cell differentiation into neuronlike cells[J]. J Cell Sci,2006,119(Pt 13):2 667
[8]Kamata Y, Hattori Y. Neural differentiation of human neuroblastoma GOTO cells via a RhoRho kinasephosphorylation signal transduction and continuous observation of a single GOTO cell during differentiation[J].J Vet Med Sci,2007,69(1):37

相似文献/References:

[1]王义生△,韩枫,曹亚伟,等.山羊骨髓间充质干细胞的体外培养与生物学特性[J].郑州大学学报(医学版),2010,(02):266.
 WANG Yisheng,HAN Feng,CAO Yawei,et al.Biologic characteristics of goat bone marrow mesenchymal stem cells cultured in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):266.
[2]张勇),程敬亮)#,李华丽),等.超顺磁性氧化铁颗粒标记对大鼠骨髓间充质干细胞活力的影响*[J].郑州大学学报(医学版),2009,(06):1176.
 ZHANG Yong),CHENG Jingliang),LI Huali),et al.Effects of super paramagnetic iron oxide labeling on biological activity of bone marrow stem cells of rat[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1176.
[3]牛德亮),常彩红)#.人工模拟胰腺微环境诱导骨髓间充质干细胞分化为胰岛素产生细胞*[J].郑州大学学报(医学版),2009,(05):960.
 NIU Deliang),CHANG Caihong).Mesenchymal stem cells transdifferentiate to insulinproducing cells upon microenvironment manipulation in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):960.
[4]蒋伟,法宪恩,李晓召.窦房结细胞裂解液对大鼠骨髓间充质干细胞的诱导分化作用[J].郑州大学学报(医学版),2011,(01):79.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):79.
[5]王义生)△,张振),张鑫),等.乙醇对兔骨髓间充质干细胞6种神经递质mRNA表达的影响*[J].郑州大学学报(医学版),2011,(03):357.
 WANG Yisheng),ZHANG Zhen),ZHANG Xin),et al.Effects of alcohol on expressions of 6 kinds of neurotransmitters mRNA in bone marrow mesenchymal stem cells of rabbits[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):357.
[6]张雁儒),张辉),马辉),等.BMP2基因转染对兔骨髓间充质干细胞碱性磷酸酶活性和钙含量的影响[J].郑州大学学报(医学版),2014,(01):102.
 ZHANG Yanru#,ZHANG Hui#,MA Hui#,et al.Effect of BMP2 gene transfection on alkaline phosphalase activity and calcium deposition in bone marrow mesenchymal stem cell of rabbits[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):102.
[7]武俊芳),牛杰),李晓鹏),等.氧化型低密度脂蛋白对小鼠骨髓间充质干细胞迁移的影响*[J].郑州大学学报(医学版),2014,(03):304.
 WU Junfang),NIU Jie),LI Xiaopeng),et al.Effect of oxidized lowdensity lipoprotein on migration of mouse bone marrowderived stem cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):304.
[8]武俊芳),牛杰),李晓鹏),等.血管紧张素Ⅱ对小鼠骨髓间充质干细胞中ERK1/2和NFκB信号途径介导的炎症反应的影响*[J].郑州大学学报(医学版),2014,(04):465.
 WU Junfang,NIU Jie,LI Xiaopeng,et al.Effect of angiotensin Ⅱ on inflammatory responses of mouse bone marrow mesenchymal stem cell mediated by ERK1/2 and NFκB pathways[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(04):465.
[9]路平),赵潇丹),郝玉娟),等.小鼠骨髓间充质干细胞在子宫内膜损伤小鼠子宫内膜中的定位*[J].郑州大学学报(医学版),2015,(01):101.
 LU Ping),ZHAO Xiaodan),HAO Yujuan),et al.Location of murine bone marrow mesenchymal stem cells in uterine endometrium of murine endometrial damage model[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):101.
[10]徐玉生,崔浩,张松,等.miRNA let7b修饰的骨髓间充质干细胞移植对大鼠脊髓损伤后脊髓NF200、GFAP表达的影响[J].郑州大学学报(医学版),2015,(03):400.
 XU Yusheng,CUI Hao,ZHANG Song,et al.Effects of miRNA let7bmodified bone mesenchymal stem cells transplantation on expressions of NF200 and GFAP in rats with spinal cord injury[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(04):400.

备注/Memo

备注/Memo:
国家自然科学基金资助项目30770758;河南省教育厅自然科学基金资助项目2008A320032
更新日期/Last Update: