[1]王贵琴,张威,陈玉,等.雷帕霉素对食管癌EC1细胞mTOR表达及细胞生长与凋亡的影响[J].郑州大学学报(医学版),2010,(05):709-712.
 WANG Guiqin,ZHANG Wei,CHEN Yu),et al.Effects of rapamycin on expression of mTOR gene,growth and apoptosis of human esophageal EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(05):709-712.
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雷帕霉素对食管癌EC1细胞mTOR表达及细胞生长与凋亡的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年05期
页码:
709-712
栏目:
食管癌研究
出版日期:
2010-09-30

文章信息/Info

Title:
Effects of rapamycin on expression of mTOR gene,growth and apoptosis of human esophageal EC1 cells
作者:
王贵琴1张威2陈玉2亚国伟1陈奎生1
1)郑州市骨科医院病理科 郑州 450052
2)郑州大学第一附属医院病理科;河南省肿瘤病理重点实验室 郑州 450052
Author(s):
WANG Guiqin1ZHANG Wei2CHEN Yu2)YA Guowei2CHEN Kuisheng2
1)Department of Pathology,Osteopathic Hospital of Zhengzhou,Zhengzhou 450052 2)Department of Pathology, the First Affiliated Hospital,Zhengzhou University;Henan Key Laboratory of Tumor Pathology, Zhengzhou 450052
关键词:
雷帕霉素食管肿瘤 EC1细胞mTOR增殖凋亡
分类号:
R735.1
摘要:
目的:探讨雷帕霉素对人食管癌EC1细胞哺乳动物雷帕霉素靶蛋白(mTOR)的表达及细胞生长、凋亡的影响。方法:采用低、中、高浓度的雷帕霉素(100、150、200 nmol/L)分别作用于EC1细胞24、48、72 h,同时设溶剂对照组和空白对照组,用免疫细胞化学及原位杂交法检测EC1细胞中mTOR蛋白与mRNA的表达,MTT法检测EC1细胞的增殖情况,TUNEL法检测各组作用24 h后细胞凋亡情况。结果:雷帕霉素作用24、48与72 h,5组细胞mTOR蛋白与mRNA的表达差异均有统计学意义(mTOR蛋白:F=29.273、34.328、41.571,P均<0.001;mTOR mRNA:F=34.969、53.614、36.943, P均<0.001),细胞生长抑制率差异亦有统计学意义(F=566.732、51.768和186.022,P均<0.001);雷帕霉素低、中、高浓度组EC1细胞mTOR蛋白与mRNA的表达均低于各对照组,且其表达随雷帕霉素浓度的增加及作用时间的延长而减弱(P<0.05)。雷帕霉素低、中、高浓度组细胞生长抑制率均高于各对照组,且随雷帕霉素浓度的增加和作用时间的延长而升高(P<0.05)。作用24 h,5组细胞凋亡指数(AI)差异有统计学意义(F=524.563,P<0.001),雷帕霉素低、中、高浓度组AI均高于各对照组,且随雷帕霉素浓度的增加而增高(P<0.05)。结论:雷帕霉素可能通过抑制EC1细胞mTOR蛋白与mRNA的表达从而抑制EC1细胞的增殖,促进细胞凋亡。

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备注/Memo

备注/Memo:
河南省杰出青年科学基金资助项目074100510009;郑州市科技创新团队基金资助项目096SYJH33097;河南省科技创新型团队基金资助项目
更新日期/Last Update: 2010-10-28