[1]郝秀轻,张林西,孙黎,等.NS398联合顺铂对Eca109细胞增殖及凋亡的影响[J].郑州大学学报(医学版),2010,(05):713-715.
 HAO Xiuqing,ZHANG Linxi,SUN Li,et al.Effects of NS398 combined with cisplatin on apoptosis and proliferation of Eca109 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(05):713-715.
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NS398联合顺铂对Eca109细胞增殖及凋亡的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年05期
页码:
713-715
栏目:
食管癌研究
出版日期:
2010-09-30

文章信息/Info

Title:
Effects of NS398 combined with cisplatin on apoptosis and proliferation of Eca109 cells
作者:
郝秀轻张林西孙黎罗强武欣范婕
河北北方学院基础医学院 张家口 075000
Author(s):
HAO XiuqingZHANG LinxiSUN LiLUO QiangWU XinFAN Jie
Basic Medical College,Hebei North University,Zhangjiakou 075000
关键词:
食管肿瘤Eca109细胞NS398顺铂凋亡增殖
分类号:
R735.1
摘要:
目的:观察环氧化物酶2选择性抑制剂NS398联合顺铂对食管癌Eca109细胞增殖及凋亡的影响。方法:采用细胞增殖抑制实验(CCK8法)检测不同质量浓度的顺铂(1.25、2.50、5.00和10.00 mg/L)单用及联合小剂量(1.00和10.00 μmol/L)NS398作用于人食管癌Eca109细胞24 h后细胞增殖抑制率;采用流式细胞仪检测细胞周期的改变及早期凋亡情况。结果:顺铂、N398单用及2者联合对Eca109细胞均有抑制增殖作用(F顺铂=1 391.300,FNS398=642.898,F交互=15.254,P均<0.001),可阻止细胞周期的进程(G0/G1期:F顺铂=1 308.300,FNS398=133.138,F交互=26.692,P均<0.001;S期:F顺铂=1 470.300,FNS398=149.638,F交互=30.000,P均<0.001),诱导细胞凋亡(F顺铂=39.493,P<0.001,FNS398=0.000,P=0.987;F交互=4.325,P=0.006)。顺铂联合NS398对Eca109细胞的作用明显强于顺铂单用,且随着NS398剂量的增高而增高(P<0.01)。结论:NS398能够增强顺铂对食管癌细胞的生长抑制和诱导凋亡效应。

参考文献/References:

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备注/Memo

备注/Memo:
河北省自然科学基金资助项目C2005000664
更新日期/Last Update: 2010-10-28