[1]骆丽,李道明.小白菊内酯对EC9706细胞增殖及VEGF、NFκB P65蛋白表达的影响[J].郑州大学学报(医学版),2010,(05):716-718.
 LUO Li),LI Daoming).Effects of parthenolide on expressions of VEGF, NFκB P65 protein and proliferation of EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(05):716-718.
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小白菊内酯对EC9706细胞增殖及VEGF、NFκB P65蛋白表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年05期
页码:
716-718
栏目:
食管癌研究
出版日期:
2010-09-30

文章信息/Info

Title:
Effects of parthenolide on expressions of VEGF, NFκB P65 protein and proliferation of EC9706 cells
作者:
骆丽12李道明1
1)郑州大学第一附属医院病理科;河南省肿瘤病理重点实验室 郑州 450052
2)北京市潞河医院病理科 北京 101149
Author(s):
LUO Li12)LI Daoming1)
1)Department of Pathology,the First Affiliated Hospital,Zhengzhou University;Henan Key Laboratory of Tumor Pathology, Zhengzhou 4500522)Department of Pathology,Beijing Luhe Hospital,Beijing 101149
关键词:
小白菊内酯EC9706细胞血管内皮生长因子核转录因子κB P65食管癌
分类号:
R735.1
摘要:
目的:探讨小白菊内酯(PTL)对食管癌EC9706细胞的增殖、血管内皮生长因子(VEGF)及核转录因子κB P65(NFκB P65)蛋白表达的影响。方法:倒置显微镜下观察不同浓度(20、40和80 μmol/L)的PTL作用48 h后EC9706细胞的形态学变化;MTT法检测20、40和80 μmol/L PTL及20 μmol/L PTL与25 mg/L 5氟尿嘧啶(5FU)联用对EC9706细胞增殖的影响;免疫细胞化学法检测20和40 μmol/L的PTL作用72 h后EC9706细胞VEGF和NFκB P65蛋白表达的改变。结果:①PTL作用EC9706细胞48 h后,细胞数量减少,细胞固缩变圆,核浓缩、核质分布不清,细胞溶解碎裂。②PTL及联合组作用于EC9706细胞24、48和72 h后,相同时间段各组细胞增殖抑制率差异均有统计学意义(χ224 h=31.420,P<0.001;χ248 h=34.590,P<0.001;χ272 h=38.190,P<0.001)。PTL对EC9706细胞的增殖有抑制作用;细胞培养72 h后PTL与5FU联合组对EC9706细胞的抑制作用与50 mg/L 5FU组相当。③PTL作用EC9706细胞后,与对照组比较VEGF和NFκB P65蛋白表达均降低(χ2 NFκB P65=25.120,P<0.001;χ2VEGF=22.190,P<0.001)。结论:PTL能抑制EC9706细胞的增殖,增加EC9706对5FU的敏感性;PTL的抗肿瘤机制可能与抑制NFκB传导通路有关。

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更新日期/Last Update: 2010-10-28