[1]李沛,赵璇,马俊芬,等.5杂氮2’脱氧胞苷和5氟尿嘧啶对食管癌细胞系中ECadherin和TIMP3基因甲基化状态及蛋白表达的影响[J].郑州大学学报(医学版),2010,(05):719-722.
 LI Pei,ZHAO Xuan,MA Junfen,et al.Effects of 5AzaCdR or 5FU on ECadherin and TIMP3 gene methylation status and their protein expressions in EC1 and EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(05):719-722.
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5杂氮2’脱氧胞苷和5氟尿嘧啶对食管癌细胞系中ECadherin和TIMP3基因甲基化状态及蛋白表达的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2010年05期
页码:
719-722
栏目:
食管癌研究
出版日期:
2010-09-30

文章信息/Info

Title:
Effects of 5AzaCdR or 5FU on ECadherin and TIMP3 gene methylation status and their protein expressions in EC1 and EC9706 cells
作者:
李沛赵璇马俊芬赵继敏杨洪艳董子明
郑州大学基础医学院病理生理学教研室 郑州 450001
Author(s):
LI PeiZHAO XuanMA JunfenZHAO JiminYANG HongyanDONG Ziming
Department of Pathophysiology, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001
关键词:
食管癌甲基化5杂氮2’脱氧胞苷5氟尿嘧啶ECadherinTIMP3EC1EC9706
分类号:
R735.1
摘要:
目的:观察去甲基化药物5杂氮2’脱氧胞苷(5AzaCdR)和5氟尿嘧啶(5FU)对食管癌细胞系EC1和EC9706(均存在ECadherin甲基化改变和TIMP3基因未发生甲基化改变)中2种基因甲基化状态及蛋白表达的影响。方法:用5AzaCdR和5FU分别作用于EC1和EC9706细胞,应用甲基化特异性PCR检测2种药物处理前后细胞中ECadherin基因和TIMP3基因的甲基化状态,应用免疫细胞化学方法检测TIMP3和ECadherin蛋白的表达。结果:5AzaCdR或5FU处理后两种食管癌细胞系中TIMP3基因仍为非甲基化;5AzaCdR作用后,两种食管癌细胞系中ECadherin基因的甲基化状态发生了逆转,而5FU作用后,ECadherin基因的甲基化状态无改变。EC1和EC9706经5AzaCdR处理后TIMP3蛋白表达无改变,而经5FU处理后表达增强(P<0.05),EC1和EC9706经两种药物处理后ECadherin蛋白表达均增强(F=116.835、119.628、191.700和210.532,P<0.001)。结论:5AzaCdR能够有效逆转EC1和EC9706细胞中ECadherin基因甲基化状态,并使其蛋白恢复表达;对TIMP3基因的甲基化状态和蛋白表达影响不大。

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备注/Memo

备注/Memo:
河南省高校科技创新人才支持计划基金资助项目2009HAST1T001;郑州大学优秀研究生培育基金资助项目A121
更新日期/Last Update: 2010-10-28