[1]郭三星),侯桂琴),李杰),等.shRNA对EC9706细胞p70S6K表达的影响[J].郑州大学学报(医学版),2011,(01):7.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(01):7.
点击复制

shRNA对EC9706细胞p70S6K表达的影响()
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年01期
页码:
7
栏目:
食管癌研究
出版日期:
2011-01-20

文章信息/Info

作者:
郭三星1)侯桂琴2)李杰3)张艳2)贝维娟2)薛乐勋13)
1)郑州大学第一附属医院肿瘤细胞研究室 郑州 450052
2)郑州大学药学院 郑州 450001
3)郑州大学生物工程系细胞生物学研究室 郑州 450001
关键词:
食管肿瘤EC9706哺乳动物雷帕霉素靶蛋白核糖体40s小亚基S6蛋白激酶短发夹RNA
文献标志码:
A
摘要:
目的:观察核糖体40 s小亚基S6蛋白激酶(p70S6K)特异性短发夹RNA(shRNA)对EC9706细胞p70S6K表达的影响。方法:根据GenBank p70S6K的mRNA序列,设计并合成p70S6K特异性的shRNA序列,退火形成双链后插入pSIRENRetroQDsRedExpress载体,构建表达载体pshRNAp70S6K,转染EC9706细胞。采用RTPCR和Western blot方法分别检测转染24、48、72、96及120 h后细胞中p70S6K mRNA和磷酸化p70S6K的表达,设未转染的EC9706细胞为对照。结果:各组细胞p70S6K mRNA和磷酸化p70S6K蛋白的表达差异有统计学意义(F=106.343,721.632,P均<0.001)。转染细胞p70S6K mRNA的表达在转染24、48和72 h后均较对照组下降(P<0.05),在第48 h表达最低;磷酸化p70S6K蛋白的表达在转染24、48、72和96 h后均较对照组下降(P<0.05),第48 h表达最低。结论:p70S6K特异性shRNA能够下调EC9706细胞中p70S6K mRNA 和蛋白的表达。

相似文献/References:

[1]庞霞,郑湘予,李晟磊,等.食管鳞癌组织中半乳糖凝集素3和Bcl2蛋白的表达*[J].郑州大学学报(医学版),2010,(01):8.
 PANG Xia,ZHENG Xiangyu,LI Shenglei,et al.Expression of Galectin3 and Bcl2 protein in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):8.
[2]张威,陈玉,亚国伟,等.RNA干扰对EC9706细胞mTOR表达及细胞生长与凋亡的影响*[J].郑州大学学报(医学版),2010,(01):11.
 ZHANG Wei,CHEN Yu,YA Guowei,et al.Effect of mTOR siRNA on the expression of mTOR gene,growth and apoptosis of human esophageal carcinoma EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):11.
[3]崔玲玲),李春阳),王旗),等.食管鳞癌组织中EphrinB2、半胱氨酸蛋白酶8蛋白和mRNA的表达*[J].郑州大学学报(医学版),2010,(01):21.
 CUI Lingling),LI Chunyang),WANG Qi),et al.Expression of EphrinB2 and Caspase8 protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):21.
[4]吴欣爱),王筱璨),樊青霞),等.食管鳞状细胞癌组织中乏氧诱导因子1α蛋白和mRNA的表达[J].郑州大学学报(医学版),2010,(02):183.
 WU Xinai),WANG Xiaocan),FAN Qingxia),et al.Expression of HIF1α protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):183.
[5]王彦平),代丽萍),吴晓冰),等.细胞色素P4501A1 Ile/Val位点基因多态性与食管癌关系的meta 分析*[J].郑州大学学报(医学版),2010,(02):186.
 WANG Yanping),DAI Liping),WU Xiaobing),et al.Metaanalysis of cytochrome P4501A1 Ile/Val gene polymorphism with esophageal carcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):186.
[6]刘欣欣,赵江沙,印宏坤,等.食管鳞状细胞癌组织中表皮调节素、双调蛋白及表皮生长因子受体mRNA的表达[J].郑州大学学报(医学版),2010,(02):189.
 LIU Xinxin,ZHAO Jiangsha,YIN Hongkun,et al.Expression of epiregulin,amphiregulin,and epidermal growth factor receptor mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):189.
[7]陈妍琼),赵志华),张蕾)#,等.食管鳞状细胞癌组织中KiSS1蛋白和微血管密度的检测*[J].郑州大学学报(医学版),2009,(06):1127.
 CHEN Yanqiong),ZHAO Zhihua),ZHANG Lei),et al.Expressions of KiSS1 protein and detection of MVD in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):1127.
[8]吴成富)),杨建萍),陈明勇),等.RNA干扰沉默桩蛋白对食管鳞癌EC9706细胞体外侵袭能力的影响*[J].郑州大学学报(医学版),2009,(06):1130.
 WU Chengfu)),YANG Jianping),CHEN Mingyong),et al.Effects of silencing expression of Paxillin using RNA interference on invasion of esophageal squamous cell carcinoma EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):1130.
[9]陈玉,亚国伟,张威,等.雷帕霉素靶蛋白RNA干扰联合雷帕霉素对人食管癌移植瘤生长和移植瘤组织中雷帕霉素蛋白基因表达的影响*[J].郑州大学学报(医学版),2009,(06):1135.
 CHEN Yu,YA Guowei,ZHANG Wei,et al.Effects of RNAi combined with rapamycin on expression of mTOR gene in tumor tissue of nude mice transplanted with human esophageal cancer cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):1135.
[10]史祖宣,李克,樊青霞#.食管鳞状细胞癌组织中神经性钙黏附蛋白和mRNA的表达*[J].郑州大学学报(医学版),2009,(06):1138.
 SHI Zuxuan,LI Ke,FAN Qingxia.Expression of Ncadherin protein and mRNA in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):1138.
[11]刘晓莉),刘怀民)#,高启龙),等.靛玉红甲肟对食管癌EC9706细胞增殖、凋亡的影响*[J].郑州大学学报(医学版),2012,(04):429.
 LIU Xiaoli,LIU Huaimin,GAO Qilong,et al.Effects of indirubin3’monoxime on proliferation and apoptosis of human esophageal cancer EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):429.

更新日期/Last Update: