[1]周舫△,梁守沛,王玉珍,等.肺癌组织中HSP701+190(G/C)多态性对HSP701 mRNA及HSP70蛋白表达的影响*[J].郑州大学学报(医学版),2011,(04):514.
 ZHOU Fang,LIANG Shoupei,WANG Yuzhen,et al.Effects of HSP701+190(G/C) polymorphisms on transcription and translation of HSP701 mRNA and HSP70 protein in lung cancer tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):514.
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肺癌组织中HSP701+190(G/C)多态性对HSP701 mRNA及HSP70蛋白表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年04期
页码:
514
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Effects of HSP701+190(G/C) polymorphisms on transcription and translation of HSP701 mRNA and HSP70 protein in lung cancer tissue
作者:
周舫1梁守沛1王玉珍1王明君2许东1姚武1吴逸明1
1)郑州大学公共卫生学院劳动卫生学教研室 郑州 450001 2)中平能化医疗集团 平顶山 467000
Author(s):
ZHOU Fang1)LIANG Shoupei1)WANG Yuzhen1)WANG Mingjun2)XU Dong1)YAO Wu1)WU Yiming1)
1)Department of Occupational Health, College of Public Health,Zhengzhou University, Zhengzhou 450001 2)Zhong Ping Energy Chemical Medicine Group,Pingdingshan 467000
关键词:
热休克蛋白70单核苷酸多态性肺癌
Keywords:
heat shock protein 70 single nucleotide polymorphism lung cancer
分类号:
R734.2
摘要:
目的:探讨热休克蛋白70(HSP70)1+190(G/C)位点基因多态性对肺癌组织中HSP701 mRNA及HSP70蛋白表达的影响。方法:应用PCRRFLP技术检测46例肺癌及相应癌旁正常组织中HSP701+190(G/C)位点多态性,RTPCR法检测HSP701 mRNA水平,Western blot测定HSP70蛋白。结果:肺癌组织中HSP701 mRNA与HSP70蛋白的表达明显高于癌旁正常组织(t=19.968和17.794, P<0.001),并且其表达水平与肺癌的分化程度有关(t=6.813,9.386,P<0.001);肺癌组织中HSP701 mRNA与蛋白表达水平呈正相关(r=0.817, P<0.001)。46例中,CC型11例(23.9%),GG型17例(37.0%),GC型18例(39.1%),不同基因型携带者HSP701 mRNA和HSP70蛋白表达水平差异均有统计学意义(F=102.607和62.617, P<0.001),CC基因型携带者表达水平高于GG和GC基因型携带者(P<0.001)。结论:HSP701+190(G/C)CC基因型可能是肺癌的一个高危险因素,并能影响其mRNA和蛋白的表达。
Abstract:
Aim:To investigate the effect of heat shock protein(HSP) 701 gene polymorphism on the expressions of HSP701 mRNA and HSP70 protein.Methods:PCRRFLP was applied to detect the single nucleotide polymorphisms (SNPs) of HSP701+190(G/C) in 46 patients with lung cancer,RTPCR was applied to measure the expression of HSP701 mRNA, and Western blot was applied to detect the level of HSP70.Results:The expression levels of HSP701 mRNA and protein in lung cancer tissue were obviously higher than those in the adjacent normal tissues(t=19.968,17.794,P<0.001), and they were related with the differentiation of lung cancer (t=6.813,9.386,P<0.001).A highly positive correlation existed between levels of HSP701 mRNA and HSP70 protein in lung concer tissue (r=0.817,P<0.001).Among the 46 cases,CC,GG and GC genotypes accounted for 23.9%,37.0%,and 39.1%,respectively.There were significant differences in the expression levels of HSP701 mRNA and HSP70 protein among the three genotypes (F=102.607,62.617, P<0.001).Compared with the other two genotypes, both the levels of HSP701 mRNA and HSP70 protein were apparently higher in the individuals with CC genotype(P<0.001).Conclusion:CC genotype of HSP701+190(G/C) may be a highrisk factor for lung cancer and affect genetic transcription and protein expression.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30600490;河南省教育厅科技创新人才支持计划基金资助项目2008HASTIT028;河南省教育厅自然科学研究基金资助项目2008A320018 △女,1974年7月生,博士,副教授,研究方向:职业肿瘤与应激医学,Email:zhoufang23@sina.com
更新日期/Last Update: 1900-01-01