[1]李双凤△,冉珂,王亚平,等.硫化氢延迟预处理对大鼠心肌缺血再灌注损伤后细胞的保护作用*[J].郑州大学学报(医学版),2011,(04):526.
 LI Shuangfeng,RAN Ke,WANG Yaping,et al.Cytoprotection of hydrogen sulfide delayed preconditioning on myocardial ischemic reperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):526.
点击复制

硫化氢延迟预处理对大鼠心肌缺血再灌注损伤后细胞的保护作用*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年04期
页码:
526
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Cytoprotection of hydrogen sulfide delayed preconditioning on myocardial ischemic reperfusion injury in rats
作者:
李双凤1冉珂1王亚平1唐正国2肖艳英1王丹1
1)中南大学湘雅二医院麻醉科 长沙 410011 2)长沙市第三医院麻醉科 长沙 410011
Author(s):
LI Shuangfeng1) RAN Ke1)WANG Yaping1)TANG Zhengguo2)XIAO Yanying1) Wang Dan1)
1)Department of Anesthesiology, the Second Xiangya Hospital, Central South University,Changsha 410011 2)Department of Anesthesiology, the Third Hospital of Changsha,Changsha 410011
关键词:
硫化氢缺血再灌注凋亡线粒体ATP敏感性钾通道大鼠 中图分类号R542.2
Keywords:
hydrogen sulfideischemia reperfusionapoptosismitochondrial ATP sensitive potassium channelrat
分类号:
R542.2
摘要:
目的:观察硫化氢(H2S)延迟预处理对大鼠心肌缺血再灌注(IR)损伤后细胞的保护作用及可能机制。方法:雄性SD大鼠32只,分为4组:假手术组(Sham组)、缺血再灌注组(IR组)、H2S延迟预处理组(H2S组)和线粒体ATP敏感性钾通道(mitoKATP)阻滞剂5HD+H2S组(5HD组),每组8只。除假手术组,余3组通过结扎在体心脏冠状动脉前降支30 min,松开复灌120 min进行IR模型制备。再灌注末电镜下观察各组大鼠心肌细胞超微结构的变化,采用免疫印迹法检测各组心肌细胞中Bcl2和Bax蛋白的表达。结果:4组间Bcl2和Bax蛋白的表达,差异有统计学意义(F=31.937和11.681, P<0.001)。与IR组相比,H2S组Bcl2蛋白上调和Bax蛋白下调(P均<0.05), 5HD组差异无统计学意义(P均>0.05)。结论:H2S延迟预处理具有心肌保护作用,其机制可能与调节细胞凋亡及激活mitoKATP有关。
Abstract:
Aim: To observe the cytoprotection of hydrogen sulfide delayed preconditioning on myocardial ischemic reperfusion injury in rats and the possible mechanism.Methods:Thirtytwo male SD rats were randomly divided into 4 groups: group sham, IR group (group IR), hydrogen sulfide treatment group(group H2S) and 5HD+H2S group(group 5HD). IR model was made by ligation of the anterior descending coronaryartery for 30 min followed by 120 min reperfusion. We observed myocardial ultrastructure under electron microscope and detected myocardial Bcl2 and Bax protein by western blotting.Results:The expressions of Bcl2 and Bax protein among 4 groups were different, there were obvious differences(F=31.937 and 11.681,P<0.001).The expressions of Bcl2 protein was higher and Bax protein was lower in group IR than in group H2S(P<0.05); there was no significant difference in group IR and in group 5HD(P>0.05).Conclusion:Hydrogen sulfide protect myocardial function. Regulation of cell apoptosis and activating mitoKATP are the possible mechanisms.

参考文献/References:

[1]Hu LF, Pan TT, Neo KL, et al. Cyclooxygenase2 mediates the delayed cardioprotection induced by hydrogen sulfide preconditioning in isolated rat cardiomyocytes[J]. Pflugers Arch,2008,455(6):971
[2]Hu Y,Chen X,Pan TT,et al.Cardioprotection induced by hydrogen sulfide preconditioning involves activation of ERK and PI3K/Akt pathway[J].Pfugers Arch,2008,455(4):607
[3]Zhao ZQ, Corvera JS, Halkos ME, et al. Inhibition of myocardial injury by ischemic postconditioning during reperfusion: comparision with ischemic preconditioning[J]. Am J Physiol Heart Circ Physiol,2003,285(2):H579
[4]汪宝军,付润芳,岳云霄,等.巴戟天寡糖对大鼠心肌缺血再灌注损伤的影响[J].郑州大学学报:医学版,2010, 45(5):792
[5]刘仰斌, 李启华,刘欣. 苦菜总黄酮对大鼠心肌缺血再灌注损伤的保护作用[J].郑州大学学报:医学版,2010,45(1):107
[6]Wang M,Tsai BM,Reiger KM. 17betaEstradiol decreases p38 MAPKmediated myocardial inflammation and dysfunction following acute ischemia [J]. J Mol Cell Cardiol, 2006, 40(2):205
[7]Dombkowski RA,Russell MJ,Olson KR.Hydrogen sulfide as an endogenous regulator of vascular smooth muscle tone in trout[J].Am J Physiol Regul Integr Comp Physiol,2004,286(4): R678
[8]Hosoki R,Matsuki N,Kimura H.The possible role of hydrogen sulfide as an endogenous smooth muscle relaxant in synergy with nitric oxide[J].Biochem BioPhys Res Commun,1997,237(3):527
[9]Elrod JW, Calvert JW, Morrison J,et al.Hydrogen sulfide attenuates myocardial ischemiareperfusion injury by preservation of mitochondrial functione[J]. Proc Natl Acad Sci USA,2007, 104(39):15560
[10]Pan TT, Chen YQ, Bian JS. All in the timing: a comparison between the cardioprotection induced by H2S preconditioning and postinfarction treatment[J]. Eur J Pharmacol,2009,616(1/3):160
[11]Hochhauser E, Kivity S, Offen D, et al. Bax ablation protects against myocardial ischemiareperfusion injury in transgeic mice[J].Am J Physiol Heart Cire Physiol, 2003, 284(6):H2351
[12]Wang N, Minatoguchi S, Chen X, et al. Antidiabetic drug miglitol inhibits myocardial apoptosis involving decreased hydroxyl radical production and Bax expression in an ischaemia/reperfusion rabbit heart[J]. Br J Pharmacol, 2004,142(6):983

相似文献/References:

[1]崔广晖)),齐宇)#,廖崇先).经冠状动脉转染转化生长因子β1基因对大鼠移植心脏缺血再灌注损伤的影响[J].郑州大学学报(医学版),2010,(01):99.
 CUI Guanghui),QI Yu),LIAO Chongxian).Effects of TGFβ1 gene transfection in vitro on ischemiareperfusion injury after heart transplantation in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):99.
[2]孙培春))△,吴万庆),吴刚),等.转化生长因子β1对大鼠肠黏膜缺血再灌注损伤的保护作用[J].郑州大学学报(医学版),2009,(06):1194.
 SUN Peichun)),WU Wanqing),WU Gang),et al.Protection of TGFβ1 against intestinal mucosal ischemiareperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):1194.
[3]艾艳秋),谢小娟),张卫)#,等.盐酸戊乙奎醚对心脏瓣膜置换术患者心肌细胞NFκB活性及血浆TNFα水平的影响[J].郑州大学学报(医学版),2009,(05):996.
 AI Yanqiu),XIE Xiaojuan),ZHANG Wei),et al.Effect of penehyclidine hydrochloride on the levels of myocardium NFκB and plasma TNFα in patients undergoing heart valve replacement[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):996.
[4]曹华,周晓红#,黄新莉,等.外源性硫化氢在脂多糖致老年大鼠急性肺损伤中的作用*[J].郑州大学学报(医学版),2010,(03):387.
 CAO Hua,ZHOU Xiaohong,HUANG Xinli,et al.Roles of exogenous hydrogen sulfide in acute lung injury induced by lipopolysaccharide in aged rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):387.
[5]马小波),杜东书),李豫),等. 大鼠胃缺血后处理模型的建立*[J].郑州大学学报(医学版),2011,(03):399.
 MA Xiaobo),DU Dongshu),LI Yu),et al.Establishment of gastric ischemic postconditioning model in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):399.
[6]王娟△,阚全程,齐跃东,等.醋柳黄酮对大鼠肾缺血再灌注损伤的保护作用[J].郑州大学学报(医学版),2011,(04):587.
 WANG Juan,KAN Quancheng,QI Yuedong,et al.Protective effects of sindacon on renal ischemicreperfusion injury in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):587.
[7]王庆治),张帮杰),王素娟),等.谷氨酰胺对大鼠急性全脑缺血再灌注损伤后小肠黏膜Occludin蛋白表达的影响[J].郑州大学学报(医学版),2011,(06):878.
 WANG Qingzhi,ZHANG Bangjie,WANG Sujuan,et al.Impact of glutamine on expression of Occludin in intestinal mucosal tissue from acute global cerebral ischemiareperfusion injury rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):878.
[8]曹慧霞,牛福坤,尤冠巧,等.硫化氢对TGFβ1诱导的HK2细胞上皮间充质转化的抑制作用[J].郑州大学学报(医学版),2012,(05):654.
 CAO Huixia,NIU Fukun,YOU Guanqiao,et al.Inhibitive effect of hydrogen sulfide on epithelialmesenchymal transition of HK2 cells induced by TGFβ1[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(04):654.
[9]方红波),张水军)#,张明),等.胆总管短暂梗阻对小鼠肾脏HSP27蛋白表达及缺血再灌注损伤的影响[J].郑州大学学报(医学版),2012,(05):662.
 FANG Hongbo),ZHANG Shuijun),ZHANG Ming),et al.Effects of transient common bile duct obstruction on expression of HSP27 and ischemiareperfusion injury in kidneys of mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(04):662.
[10]程鹤云,滕军放#,郭利利.重组人粒细胞集落刺激因子对急性缺血再灌注脑损伤大鼠脑组织Egr1、Survivin蛋白表达及血管再生的影响[J].郑州大学学报(医学版),2013,(02):200.
 CHENG Heyun,TENG Junfang,GUO Lili.Effect of rhGCSF on Egr1 and Survivin expressions and vascular regeneration of rats with acute cerebral ischemia reperfusion injury[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):200.

备注/Memo

备注/Memo:
*湖南省卫生厅基金资助项目B2009110 △女,1984年9月生,硕士,住院医师,研究方向:心肌保护,Email:lishuangfeng1984@126.com
更新日期/Last Update: 1900-01-01