[1]贾莉婷△,刘艳丽,马葆靖,等.两种多囊卵巢综合征大鼠模型造模方法的比较*[J].郑州大学学报(医学版),2011,(04):538.
 JIA Liting,LIU Yanli,MA Baojing,et al.Comparison of two kinds of polycystic ovary syndrome rat models[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):538.
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两种多囊卵巢综合征大鼠模型造模方法的比较*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年04期
页码:
538
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Comparison of two kinds of polycystic ovary syndrome rat models
作者:
贾莉婷1刘艳丽1马葆靖2张展1李肖甫1赵德华1张秋菊1郭瑞莹1宋晓妍1荣守华1
1)郑州大学第三附属医院检验科 郑州 450052 2)郑州大学第三附属医院保健部 郑州 450052
Author(s):
JIA Liting1)LIU Yanli1)MA Baojing2)ZHANG Zhan1)LI Xiaofu1)ZHAO Dehua1)ZHANG Qiuju1)GUO Ruiying1)SONG Xiaoyan1) RONG Shouhua1)
1)Clinical Laboratory,the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 2)Department of Heath Care,the Third Affiliated Hospital,Zhengzhou University, Zhengzhou 450052
关键词:
多囊卵巢综合征来曲唑脱氢表雄酮动物模型雄激素受体黄体生成素受体大鼠
Keywords:
polycystic ovaries syndromeletrozoledehydroepiandrosteroneanimal modelandrogen receptorluteinizing hormone receptorrat
分类号:
R711.75
摘要:
目的:采用来曲唑和脱氢表雄酮(DHEA)建立多囊卵巢综合征(PCOS)大鼠模型,并对2种模型进行比较。方法:6周龄和23日龄SD雌性大鼠各60只分别用于来曲唑和DHEA建模,均随机分为实验组、溶剂对照组和空白对照组各20只;从建模前后大鼠体质量增长、动情周期、卵巢变化、血清性激素水平、卵巢中雄激素受体(AR)和黄体生成素受体(LHR)等指标进行模型评价。结果:①2种造模方法的实验组大鼠卵巢颗粒细胞层明显减少,间质增生。②来曲唑造模的实验组实验前后体质量增加量、卵巢体积和相对质量均大于对照组(F=13.136,9.778和6.544,P均<0.05);DHEA造模的实验组仅卵巢体积小于对照组 (F=19.383,P<0.001)。③来曲唑造模的实验组黄体生成素、睾酮(T)含量高于对照组,而卵泡刺激素(FSH)、雌二醇(E2)、孕酮(P)低于对照组(P<0.05);DHEA造模组的实验组血清T、E2高于对照组,FSH低于对照组(P<0.05)。④来曲唑造模的实验组AR、LHR含量均高于对照组(P<0.05);DHEA造模的实验组仅AR高于对照组(P<0.05)。结论:①来曲唑大鼠模型更接近于人PCOS特征,是相对理想的PCOS模型。②AR、LHR参与了多囊状卵巢的形成,可作为PCOS模型蛋白质水平的评价指标。
Abstract:
Aim:To compare two kinds of polycystic ovaries syndrome rat models induced by letrozole and dehydroepiandrosterone.Methods:Rats aged six weeks and 23 days(each 60 cases) were administered letrozole and DHEA respectively to induce PCOS model.Increased weight of rats,oestrous cycle,morphology and histology of ovary,serum hormone levels and the expression of AR,LHR in the ovary were examined.Results:①The two experimental groups showed less granular cell layer and proliferated mesenchyma in ovary. ②The increased weight, volume,and relative quality of ovaries in letrozole experimental groups were larger than that in control(F=13.136,9.778 and 6.544,P<0.05);In DHEA group, the volume of ovrian in the experimental group was significantly smaller than in controls(F=19.383,P<0.001).③The level of luteinizing hormone(LH) and testosterone(T) of letrozole experimental group were significantly higher than in controls, serum follicle stimulating hormone(FSH), estradiol(E2) and progesterone(P) of letrozole experimental group was significantly lower than controls.T and E2 of DHEA experimental group were significantly higher than controls, FSH of DHEA experimental group were significantly lower than controls(P<0.05).④The expression of AR,LHR in the ovary of letrozole experimental group were significantly higher than in controls(P<0.05). In DHEA group, the expression of AR in the ovary was significantly higher than in controls(P<0.05).Conclusion: ①Compared with DHEA groups, PCOS model induced by letrozole in several ways is more similar to the human disease and it is a relative perfect rat model. ②The expression of AR,LHR in rat ovary can be evaluated indexes for protein level in PCOS model.

参考文献/References:

[1]Yavasoglu I, Kucuk M, Coskun A,et al. Polycystic ovary syndrome and prolactinoma association[J]. Inter Med,2009,48(8):611
[2]胡颜霞,张展,贾莉婷,等. 来曲唑诱导多囊卵巢综合征大鼠模型的研究[J].中国妇幼保健,2006,21(7):957
[3]任伟慧,贾莉婷,张展,等.来曲唑诱导的多囊卵巢综合征大鼠卵巢组织中角质细胞生长因子的表达[J].郑州大学学报:医学版,2008,43(3):532
[4]郭洪胜,安长新,陈东.来曲唑诱导多囊卵巢综合征大鼠卵巢组织中FSH受体的表达及其意义[J].吉林大学学报:医学版,2009,35(6):1048
[5]Aytan H,Caglar P,Uygur D, et al.Effect of the immunomodulator leflunomide on the induction of endometriosis in an experimental rat model[J].Fertil Steril,2007,87(3):698
[6]Kafali H, Iriadam M, Ozardali I,et al.Letrozoleinduced polycystic ovaries in the rat: a new model for cystic ovarian disease[J].Arch Med Res,2004,35(2):103
[7]Meenakumari KJ, Agarwal S, Krishna A, et al. Effects of metformin treatment on luteal phase progesterone concentration in polycystic ovary syndrome[J]. Braz J Med Biol Res,2004,37(11):1637
[8]Abbott DH, Dumesic DA,Franks S.Developmental origin of polycystic ovary syndromea hypothesis[J].J Endocrinol,2002,174(1):1
[9]Manneras L, Cajander S, Holmang A,et al.A new rat model exhibiting both ovarian and metabolic characteristics of polycystic ovary syndrome[J].Endocrinology,2007,148(8): 3781
[10]Zurvarra FM,Salvetti NR,Mason JI,et al.Disruption in the expression and immunolocalisation of steroid receptors and steroidogenic enzymes in letrozoleinduced polycystic ovaries in rat[J].Reprod Fertil Dev,2009,21(7):827
[11]Jakimiuk AJ, Weitsman SR, Navab A,et al. Luteinizing hormone receptor, steroidogenesis acute regulatory protein, and steroidogenic enzyme messenger ribonucleic acids are overexpressed in thecal and granulosa cells from polycystic ovaries[J].J Clin Endocrinol Metab,2001,86(3):1318

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备注/Memo

备注/Memo:
*河南省医学科技攻关基金资助项目200803068;河南省科技攻关基金资助项目092102310185;河南省教育厅自然科学研究基金资助项目2010A310014 △女,1958年11月生,本科,教授,研究方向:临床免疫学,Email: jialt@163.com
更新日期/Last Update: 1900-01-01