[1]徐晓杰,周鸣,李桂源#.bromodomain结构域对溴区包含蛋白7亚细胞定位的影响*[J].郑州大学学报(医学版),2011,(04):542.
 XU Xiaojie,ZHOU Ming,LI Guiyuan.Effects of bromodomain on subcellular localization of BRD7[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):542.
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bromodomain结构域对溴区包含蛋白7亚细胞定位的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年04期
页码:
542
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Effects of bromodomain on subcellular localization of BRD7
作者:
徐晓杰1周鸣2李桂源2#
1)南阳医学高等专科学校病理学教研室 南阳 473000 2)中南大学湘雅医学院肿瘤研究所分子遗传室 长沙 410078
Author(s):
XU Xiaojie1)ZHOU Ming2)LI Guiyuan2)
1)Department of Pathology, Nanyang Medical School,Nanyang 4730002)Department of Molecular Genetics,Cancer Research Institute,Xiangya School of Medicine, Central South University, Changsha 410078
关键词:
鼻咽癌缺失突变亚细胞定位bromodomain溴区包含蛋白7
Keywords:
nasopharyngeal carcinomadeletion mutationsubcellular localizationbromodomainBRD7
分类号:
R739.6
摘要:
目的:探讨溴区包含蛋白7(BRD7)的亚细胞定位以及bromodomain结构域对BRD7亚细胞定位的影响。方法:首先采用GFP介导的亚细胞定位方法,在非洲绿猴肾COS7细胞中考察BRD7的亚细胞定位;然后分别构建bromodomain缺失型的BRD7重组体pCMVMyc/BRD7△brd和pEGFPC2/BRD7△brd, 并通过GFP介导的亚细胞定位方法和间接免疫荧光方法,分别在COS7中考察bromodomain缺失型BRD7的亚细胞定位,从而探讨BRD7的bromodomain结构域对其亚细胞定位的影响。结果:成功构建了pCMVMyc/BRD7△brd和pEGFPC2/BRD7△brd。BRD7 及bromodomain缺失型BRD7在COS7中均主要定位在细胞核,与染色质的分布存在一定程度的相似性。结论:bromodomain结构域的缺失并不影响BRD7的细胞核分布,但是在核内的分布模式发生了一定的改变。
Abstract:
Aim:To investigate the subcellular localization of BRD7 and the effects of bromodomain on its localization.Methods:Firstly, the subcelluar localization of BRD7 was detected in COS7 cells by direct GFP fluorescence.Secondly, bromodomain deleted BRD7 mutations, pCMVMyc/BRD7△brd and pEGFPC2/BRD7△brd, were constructed to investigate the effects of bromodomain on the subcellular localization of BRD7 in COS7 cells by GFP fluorescence and indirect immunofluorescence.Results: It was found that BRD7 and bromodomain deleted BRD7 mutations were all mainly localized in nucleus, which was similar to the nuclear distribution of chromatin, to some extent.Conclusion: Bromodomain deletion may not affect the nuclear localization of BRD7,but the localization pattern of BRD7 in nucleus is changed.

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30300175, 30200135, 30400238, 30470367 #通讯作者,男,1951年11月生,博士,教授,研究方向:鼻咽癌的分子遗传学,Email: ligy@xysm.net
更新日期/Last Update: 1900-01-01