[1]朱春晖,谢勇,吕农华#.外源性野生型p53基因对sw1990细胞调控基因表达的影响[J].郑州大学学报(医学版),2011,(04):559.
 ZHU Chunhui,XIE Yong,LV Nonghua.Influence of exogenous wildtype p53 on proliferation and expression of related genes of sw1990 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):559.
点击复制

外源性野生型p53基因对sw1990细胞调控基因表达的影响
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年04期
页码:
559
栏目:
论著
出版日期:
2011-07-15

文章信息/Info

Title:
Influence of exogenous wildtype p53 on proliferation and expression of related genes of sw1990 cells
作者:
朱春晖谢勇吕农华#
南昌大学第一附属医院消化研究所 南昌 330006
Author(s):
ZHU ChunhuiXIE YongLV Nonghua
Gastrointestinal Research Institute,the First Affiliated Hospital,Nanchang University,Nanchang 330006
关键词:
p53转染胰腺癌sw1990细胞
Keywords:
p53transfectionpancreatic carcinomasw1990 cell
分类号:
R576
摘要:
目的:探讨外源性野生型p53基因对胰腺癌SW1990细胞调控基因表达的影响与可能机制。方法:分别采用RTPCR和Western blot方法检测sw1990细胞、重组质粒p53GFP转染的sw1990细胞和正常胰腺组织中p53、p21waf1/cip1、bax、 puma、pten 及mdm2 mRNA和蛋白的表达。结果:sw1990细胞中p53、p21waf1/cip1、bax、puma和pten mRNA的表达低于正常胰腺组织(t分别为3.657、4.214、5.463、2.225和2.032,P均<0.05),mdm2 mRNA的表达高于正常胰腺组织(t=4.262,P=0.017),P53、BAX、PTEN和磷酸化AKT蛋白水平低于正常胰腺组织(t分别为7.928、5.879、10.287和4.798,P均<0.05)。p53GFP转染组中p53、p21waf1/cip1、bax、puma、pten和mdm2 mRNA的表达高于未处理组(t分别为3.512、4.227、2.310、2.410、5.476和3.285,P均<0.05) ,P53、BAX和PTEN蛋白水平高于未处理组(t分别为16.040、7.885和2.898,P均<0.05)。结论:外源性野生型p53基因导入后可能通过诱导p21waf1/cip1、bax、pten、puma和mdm2的表达。
Abstract:
Aim: To discuss the influence of exogenous wildtype p53 gene on the proliferation of pancreatic carcinoma, as well as the expression of related genes.Methods:RTPCR and western blotting was performed to detect the mRNA expression and protein level of p53,p21waf1/cip1,bax,puma ,pten and mdm2 in sw1990, sw1990 transfected with recombinant plasmids p53GFP and normal pancreatic tissue.Results:p53,p21waf1/cip1 ,bax,puma and pten mRNA expression in sw1990 is lower than that in normal pancreatic tissue(t value was 3.657,4.214,5.463,2.225,and 2.032,P<0.05),mdm2 mRNA expression is higher than that in normal pancreatic tissue (t=4.262,P=0.017);the protein content of P53、BAX、PTEN and pAKT is lower than that in normal pancreatic tissue (t value was 7.928,5.879,10.287,and 4.798,P<0.05);p53, p21waf1/cip1, bax, puma,pten and mdm2 mRNA expression in the p53GFP transfection group was higher than that in the untreated group(t value was 3.512,4.227,2.310,2.410,5.476,and 3.285,P<0.05),P53,BAX,PTEN protein level in the p53GFP transfection group was higher than that in the untreated group(t value was 16.040,7.885,and 2.898,P<0.05).Conclusion:Exogenous wildtype p53 gene can inhibit the proliferation of sw1990 possibly through the induction of the expression of p21waf1/cip1,bax,pten,puma and mdm2.

参考文献/References:

[1]Jemal A,Siegel R,Ward E,et al.Cancer statistics,2008[J].CA Cancer J Clin,2008,58(2):71
[2]徐刚,刘占举,崔静,等.p53和bcl2在胰腺癌组织中的表达及意义[J].河南科技大学学报:医学版,2007,25(1):5
[3]Kaina B.DNA damagetriggered apoptosis: critical role of DNA repair,double strand breaks,cell proliferation and signaling[J].Biochem Pharmacol,2003,66(8):1547
[4]Hofmann TG,Moller A,Sirma H,et al.Regulation of p53 activity by its interaction with homeodomaininteracting protein kinase2[J].Nat Cell Biol,2002,4(1):1
[5]Pan ZQ,Reardon JT,Li L,et al. Inhibition of nucleotide excision repair by the cyclindependent kinase inhibitor p21[J].J Biol Chem,1995,270(37):22008
[6]Cory S,Adams JM. The Bc12 family: regulators of the cellular lifeordeath switch[J].Nat Rev Cancer,2002,2(9):647
[7]Bomer C.The Bcl2 protein family:sensors and checkpoints for lifeordeath decisions[J].Mol Immunol,2003,39(11):615
[8]Nakano K,Vousden KH. PUMA,a novel proapoptotic gene is induced by p53[J].Mol Cell,2001,7(3):683
[9]Yu J,Zhang L,Hwang PM,et al. PUMA induces the rapid apoptosis of colorectal cancer cell[J].Mol Cell,2001,7(3):673
[10]Oda K,Arakawea H,Tanaa T,et al.p53AIPI,a potential mediator of p53 dependent apoptosis,and its regulation by Ser462 phosphorylated p53[J].Cell,2000,102(6):849
[11]Shimizu S,Narita M,Tsujimoto Y. Bcl2 family proteins regulate the release of apoptogenic cytochrome C by the mitochondrial channel VDAC[J].Nature,1999,399(6735):483
[12]Oda E,Ohki R,Murasawa H,et al.Noxa,a BH3only member of the Bcl2 family and candidate mediator of p53induced apoptosis[J].Science,2000,288(5468):1053
[13]Gericke A,Munson M,Ross AH.Regulation of the PTEN phosphatase[J].Gene,2006, 347:1
[14]Weng L,Brown J,Eng C.PTEN induces apoptosis and cell cycle arrest through phosphoinositol3kinase/Aktdependent and independent pathways[J].Hum Mol Genet,2001,10(3):237
[15]Dinulescu DM,Ince TA,Quade BJ,et al.Role of Kras and Pten in the development of mouse models of endometriosis and endometrioid ovarian cancer[J].Nat Med,2005, 11(1):63
[16]Maknio K,Day CP,Wang SC,et al.Upregulation of IKKα/IKKβ by integrinlinked kinase is required for HER2/nueinduced NFκB antiapoptotic pathway[J].Oncogene,2004,23(21):3883
[17]Weng LP,Brown JL,Baker KM,et al.PTEN blocks insulinmediate EST2 phosphorylation through MAP kinase,independently of the phosphoinositide 3kinase pathway[J].Hum Mol Genet,2002,11(15):1687

相似文献/References:

[1]陈奎生,王志永,周强,等.真核起始因子4E反义寡核苷酸对人食管癌EC1细胞中真核起始因子4E表达的影响*[J].郑州大学学报(医学版),2009,(05):935.
 CHEN Kuisheng,WANG Zhiyong,ZHOU Qiang,et al.Effect of antisense oligodeoxynucleotides of eukaryotic initiation factor 4E on eIF4E expression in human esophageal carcinoma EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):935.
[2]张艳芳),徐发林),张俊河),等.甲泼尼龙对心肌缺血再灌注损伤大鼠心肌组织中p53、caspase3mRNA表达的影响*[J].郑州大学学报(医学版),2009,(02):321.
 ZHANG Yanfang),XU FaLin),ZHANG Junhe),et al.Influence of methylprednisolone on expressions of p53 and caspase3 mRNA in myocardia tissue of rats with ischemia reperfusion injury[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):321.
[3]樊慧),王立东)#,李吉林),等.同卵双胞胎食管、贲门组织P53、PCNA蛋白的表达*[J].郑州大学学报(医学版),2009,(01):34.
 FAN Hui),WANG Lidong),LI Jilin),et al.Expression of P53 and PCNA in esophageal and gastric cardia epithelia tissue from the monozygotic twins[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):34.
[4]叶柯)△,葛红),蒋月),等.放疗前后食管鳞状细胞癌组织中P53和Caspase3蛋白的表达*[J].郑州大学学报(医学版),2009,(01):45.
 YE Ke),GE Hong),JIANG Yue),et al.Expression of P53 and Caspase3 in esophageal squamous cell carcinoma tissue before and after radiotherapy[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):45.
[5]李留霞),乔玉环)#,王淼),等.人磷脂磷酸水解酶3基因真核表达载体的构建及其对卵巢癌细胞生长的抑制作用*[J].郑州大学学报(医学版),2009,(01):69.
 LI Liuxia),QIAO Yuhuan),WANG Miao),et al.Construction of hLPP3 eukaryotic expression vector and its inhibition effect on ovarian cancer cell line[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(04):69.
[6]王威,郝长付,吴拥军#,等.p53 Arg/Pro基因多态性及吸烟对河南汉族人群肺癌发病的影响[J].郑州大学学报(医学版),2010,(05):746.
 WANG Wei,HAO Changfu,WU Yongjun,et al.Effects of p53 Arg/Pro gene polymorphism and smoking on the occurrence of lung cancer in Henan Han population[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(04):746.
[7]周胜理),王立东)﹟,岳文彬),等.同卵双胞胎食管鳞状细胞癌患者7对临床病理特征[J].郑州大学学报(医学版),2011,(01):123.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):123.
[8]朱春晖),谢勇),吕农华)#.5氟尿嘧啶对胰腺癌细胞sw1990增殖及相关基因表达的影响[J].郑州大学学报(医学版),2011,(06):887.
 ZHU Chunhui),XIE Yong),LV Nonghua).Influence of 5fluorouracil on proliferation and expression of associated genes of sw1990[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):887.
[9]罗向阳△,王伟.尿道下裂患儿包皮组织p53和p21waf/cip1 mRNA的表达[J].郑州大学学报(医学版),2011,(06):898.
 LUO Xiangyang,WANG Wei.Expressions of p53 and p21waf/cip1 mRNA in preputial tissue of children with hypospadias[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(04):898.
[10]贺红柳),轩小燕),李刚),等.稳定表达磷酸化Stat3蛋白的EC1细胞株的获取*[J].郑州大学学报(医学版),2013,(03):297.
 HE Hongliu),XUAN Xiaoyan),LI Gang),et al.Establishment of EC1 cell line stably expressing pStat3 protein[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(04):297.

备注/Memo

备注/Memo:
#通讯作者,女,1954年9月生,硕士,教授,研究方向:消化系统疾病的早期诊治,Email:lvnonghua@163.com
更新日期/Last Update: 1900-01-01