[1]洪永贵△,黄涛,郑玉玲,等.养阴清热法对放疗后食管癌大鼠cmyc蛋白表达和细胞凋亡的影响*[J].郑州大学学报(医学版),2011,(05):667.
 HONG Yonggui,HUANG Tao,ZHENG Yuling,et al.Effects of nourishingyin and clearingheat therapy on cmyc expression and apoptosis of esophageal cancerous rats after radiotherapy[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):667.
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养阴清热法对放疗后食管癌大鼠cmyc蛋白表达和细胞凋亡的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
667
栏目:
食管癌研究
出版日期:
2011-09-20

文章信息/Info

Title:
Effects of nourishingyin and clearingheat therapy on cmyc expression and apoptosis of esophageal cancerous rats after radiotherapy
作者:
洪永贵1黄涛2郑玉玲3王俊生1
1)安阳市肿瘤医院内科 安阳 455000 2)黄河科技学院医学院 郑州 450005 3)河南中医学院 郑州 450008
Author(s):
HONG Yonggui1HUANG Tao2ZHENG Yuling3WANG Junsheng1
1)Department of Internal Medicine, Anyang Tumor Hospital, Anyang 455000 2)College of Medical Sciences,Huanghe Science and Technology College,Zhengzhou 450005 3)Henan University of Traditional Chinese Medicine, Zhengzhou 450008
关键词:
食管肿瘤养阴清热法凋亡cmyc大鼠
分类号:
R735.1
摘要:
目的:探讨养阴清热法对放疗后食管癌大鼠cmyc蛋白表达及细胞凋亡的影响。方法:130只Wistar大鼠,20只为空白对照组(空白组),余110只用药物诱发食管癌,1次/周,连续20周,经病理证实造模成功后,20只作为食管癌造模组(食管癌组),余90只局部60Co照射5 d后分为5组:单放组[蒸馏水10 mL/(kg·d)]、六味组[六味地黄丸4.5 g/(kg·d)]、低剂量组[地黄管食通口服液5 g/(kg·d)]、中剂量组[地黄管食通口服液10 g/(kg·d)]和高剂量组[地黄管食通口服液15 g/(kg·d)],灌胃5周,完成后处死大鼠,SP法检测大鼠食管癌组织cmyc蛋白的表达,TUNEL法检测细胞凋亡,并计算凋亡指数。结果:①cmyc表达:7组比较,差异有统计学意义(H=22.038,P=0.001)。②凋亡:各组均见凋亡,凋亡指数比较差异有统计学意义(F=2 958.293,P<0.001);各治疗组凋亡指数均高于空白组及食管癌组(P均<0.001);六味组与低剂量及单放组相比差异无统计学意义(P分别为0.055和0.954);其余各药物组相比差异均有统计学意义(P均<0.001)。结论:养阴清热法可能通过抑制cmyc基因突变诱导食管癌细胞凋亡。

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备注/Memo

备注/Memo:
*国家中医药管理局基金资助项目0203JP25
△男,1969年8月生,硕士,副主任医师,研究方向:恶性肿瘤的中西医结合治疗,Email:hygsir168@126.com
更新日期/Last Update: 2011-09-20