[1]刘章锁△,王建军.ANGPTL3反义寡核苷酸对EC9706细胞侵袭黏附能力的影响*[J].郑州大学学报(医学版),2011,(05):670.
 LIU Zhangsuo,WANG Jianjun.Influence of ANGPTL3 antisense oligodeoxynucleotide on ability of invasion and adhesion of EC9706 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):670.
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ANGPTL3反义寡核苷酸对EC9706细胞侵袭黏附能力的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
670
栏目:
食管癌研究
出版日期:
2011-09-20

文章信息/Info

Title:
Influence of ANGPTL3 antisense oligodeoxynucleotide on ability of invasion and adhesion of EC9706 cells
作者:
刘章锁12王建军2
1)河南省煤炭总医院胸外科 郑州 450011 2)华中科技大学同济医学院附属协和医院胸外科 武汉 430022
Author(s):
LIU Zhangsuo12WANG Jianjun2
1)Department of Chest Surgery,the Coal General Hospital of Henan Province, Zhengzhou 450011 2)Department of Chest Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022
关键词:
ANGPTL3反义寡核苷酸EC9706细胞侵袭黏附
分类号:
R735.1
摘要:
目的:观察ANGPTL3反义寡核苷酸(ASODN)对食管癌细胞EC9706侵袭黏附能力的影响。方法:根据GenBank收录的ANGPTL3 mRNA序列,设计ASODN,并制成脂质体ASODN混合液转染EC9706细胞,观察转染后EC9706细胞黏附率、细胞侵袭穿膜细胞数及侵袭抑制率。结果:ANGPTL3 ASODN转染48 h后,EC9706细胞的分布较对照组明显稀疏,细胞体积变小,皱缩呈圆形,胞核及胞质轮廓折射增强,较粗糙,部分细胞不再贴壁生长,悬浮于培养液中。ASODN组细胞的黏附率较对照组和SCODN组降低(P<0.05),侵袭细胞数下降(P<0.05),侵袭抑制率增加(t=124.470,P<0.001)。结论:ANGPTL3 ASODN能降低EC9706细胞侵袭黏附能力,从而影响恶性肿瘤浸润转移生物学行为。

参考文献/References:

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备注/Memo

备注/Memo:
*河南省基础与前沿技术研究计划基金资助项目102300410057
△男,1963年7月生,博士,主任医师,研究方向:胸部肿瘤,Email: liuzs666@sina.com
更新日期/Last Update: 2011-09-20