[1]苏建华△,林伟,陈玉芳,等.丁咯地尔对大鼠坐骨神经嵌压伤后背根神经元凋亡及血管内皮生长因子表达的影响*[J].郑州大学学报(医学版),2011,(05):674-22.
 SU Jianhua,LIN Wei,CHEN Yufang,et al.Effects of buflomedil on neural cell apoptosis and expression of vascular endothelial growth factor in dorsal root ganglionic following crushing lesion of sciatic nerve in rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):674-22.
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丁咯地尔对大鼠坐骨神经嵌压伤后背根神经元凋亡及血管内皮生长因子表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
674-22
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Effects of buflomedil on neural cell apoptosis and expression of vascular endothelial growth factor in dorsal root ganglionic following crushing lesion of sciatic nerve in rats
作者:
苏建华1林伟1陈玉芳2丁新生3唐金荣3肖杭4包德诚1
1)江苏大学附属金坛医院神经内科 金坛213200 2)常州市第四人民医院病理科 常州213001 3)南京医科大学第一附属医院神经内科 南京210029 4)南京医科大学应用神经毒理研究所 南京210029
Author(s):
SU Jianhua1LIN Wei1CHEN Yufang2DING Xinsheng3TANG Jinrong3XIAO Hang4BAO Decheng1
1)Department of Neurology,the Affiliated Jintan Hospital,Jiangsu University,Jintan 213200 2)Department of Pathology,the Fourth People's Hospital of Changzhou,Changzhou 213001 3)Department of Neurology,the First Affiliated Hospital,Nanjing University of Medical Sciences,Nanjing 210029 4)Institute of Toxicology and Neurology, Nanjing University of Medical Sciences,Nanjing 210029
关键词:
丁咯地尔坐骨神经嵌压伤背根神经元凋亡血管内皮生长因子大鼠
Keywords:
buflomedilsciatic nerve crush injuryneuron of dorsal root ganglionicapoptosisvascular endothelial growth factorrat
分类号:
R363
摘要:
目的:研究丁咯地尔对大鼠坐骨神经嵌压伤后背根神经元凋亡及血管内皮生长因子(VEGF)表达的影响。方法:84只成年健康SD大鼠分为正常组、模型组[生理盐水,0.8 mL/(kg·d)]、丁咯地尔组[丁咯地尔,40 mg/(kg·d)],腹腔注射给药14 d后制作坐骨神经嵌压伤动物模型,模型制备后6 h、12 h、24 h、48 h、72 h、96 h和7 d提取L1~5背根神经节,采用TUNEL法检测神经细胞的凋亡,应用免疫组化方法检测VEGF的表达。结果:正常组少见背根神经细胞凋亡;模型组和丁咯地尔组在模型制备后各时间点神经细胞凋亡数差异有统计学意义(F=13.790和23.084,P<0.001),模型组12 h神经细胞凋亡数较正常组明显增多,72 h达高峰,7 d减弱;丁咯地尔组12 h神经细胞凋亡增多,但较模型组减少,24 h~7 d均较同期模型组明显减少。正常组VEGF阳性神经细胞见少量表达;模型组和丁咯地尔组在模型制备后各时间点VEGF阳性神经细胞数差异有统计学意义(F=90.670和70.430,P<0.001),模型组在48 h有较高表达,持续至72 h,与正常组比较差异均有统计学意义(P<0.05);丁咯地尔组在模型制备后24 h表达升高,48~72 h表达到高峰,持续至96 h。以上各时间点丁咯地尔组神经细胞凋亡数和VEGF阳性细胞数与模型组比较差异有统计学意义(P<0.05)。结论:坐骨神经嵌压伤后背根神经元存在广泛的细胞凋亡现象,丁咯地尔可能通过上调VEGF来抑制坐骨神经损伤后背根神经元的凋亡。
Abstract:
Aim: To explore effect of buflomedil on the apoptosis of the neuron and the expression of vascular endothelium growth factor(VEGF) in dorsal root ganglionic(DRG) following crushing lesion of sciatic nerve in rats.Methods:A total of 84 SD rats were divided into the normal group,model group[normal saline,0.8 mL/(kg·d)],and buflomedil group [40 mg/(kg·d)] in random,Drugs were given to each group except normal group by intrapritoneal injection once per day for 14 days, then the sciatic nerve lesion models were performed, the apoptosis of the neuron cells was detected using the TUNEL methods,and the expression of VEGF was detected by immunohistochemical staining at the end of 6 h, 12 h, 24 h, 48 h, 72 h, 96 h, 7 days,respectively.Results:There was less TUNEL positive cells and expression of VEGF in normal group;at the end of 12 h after the model perfomed, the apoptosis of the neural cells in model group increased obviously than the buflomedil group, peaked at 72 h after the sciatic nerve lesion, and it decreased at the end of 7 days(F=13.790,P<0.001);and also the apoptosis of the neural cells in buflomedil group increased, compared with the model group at the end of 12 h, decreased obviously at the end of 24 h~7d, compared with model group(P<0.05).There was less expression of VEGF in normal group; the expressions of VEGF increased in the model group at the end of 48 h after the model perfomed,lasted to 72 h(P<0.05);and also the expression of VEGF in buflomedil group increased obviously at the end of 24 h(P<0.05),peaked at 48~72 h, lasted to 96 h(F=70.430,P<0.001), Compared with model group,the expression of VEGF were obviously different at the above times(P<0.05).Conclusion:Buflomedil could upregulate the expression of VEGF, and performs neuroprotective effect by inhibiting apoptosis.

参考文献/References:

[1]Keane RW,Davis AR,Dietrich WD.Inflammatory and apoptotic signaling after spinal cord injury[J].Neurotrauma,2006,23(3/4):335
[2]Jin KL,Mao XO,Greenberg DA.Vascular endothelial growth factor:direct neuroprotective effect in vitro ischemia[J]. PNAS, 2000, 97(18):10242
[3]Sondell M,Lundborg G,Kanje M.Vascular endothelial growth factor has neurotrophic activity and stimulates axonal outgrowth, enhancing cell survival and Schwann cell proliferation in the peripheral nervous system [J].Neurosic,1999,19(14):5731
[4]Marteau P,Ballet F,Chretien Y,et al.Effect of vasodilators on hepatic microcirculation:a study of the inhibition of norepinephrineinduced vasoconstriction in the isolat perfused rat liver[J].Hepatology,1988,8(2):228
[5]Boisseau MR,Freyburger G,Belloc F,et al.Hemorheological effects buflomedil:action on shape and functions of the human neutrophils[J].Blood Vessels,1991,28(Suppl 1):15
[6]苏建华,徐厚明,唐金荣,等.盐酸丁咯地尔对周围神经损害的保护作用[J].中国新药杂志,2004,13(12):1322
[7]Patro IK, Hattopadhyay M, Patrio N.Flunarizine enhances functional recovery following sciatic nerve crush lesion in rats [J]. Neurosci Lett,1999,26(3):97
[8]谭振军,魏劲波,李之望,等.催产素对大鼠背根神经节分离细胞GABA激活电流的调制作用[J].生理学报,2000,52(5):381
[9]Calaterra TC,Rand RW,Bentson JR.Ischemic paralysis of the facial nerve:a possible etiologic factor in Belys palsy[J]. Laryngoscope,1976,86(1):92
[10]Dahlin LB.Aspects on pathophysiology of nerve entrapments and nerve compression injuries[J].Neurosurg Clin N Am,1991,2(1):21
[11]Maede K,Chung YS,Ogawa Y,et al.Prognostic value of vascular endothelial growth factor expression in gastric carcinoma [J].Cancer,1996,77(5):858
[12]Storkebaum E,Lambrechts D,Carmeliet P.VEGF once regarded as a specific angiogenic factor,now implicated inneuroprotection[J].Bioassays,2004,26(9):943
[13]苏建华,章莉萍,陈玉芳,等.盐酸丁咯地尔预处理对大鼠坐骨神经损害后神经元血管内皮生长因子的影响 [J].徐州医学院学报,2006,26(2):105
[14]Sun FY,Guo X.Molecular and cellular mechanisms of neuroprotection by vascular endothelium growth factor[J].J Neurosci Res,2005,79(1/2):180

备注/Memo

备注/Memo:
*常州市科技局指导性科研基金资助项目CS2008928;金坛市科技局基金资助项目TS2007069;金坛市“133工程”基金资助项目2008 △男,1969年11月生,本科,副主任医师,副教授,研究方向:周围神经损伤和缺血再灌注损伤,Email:sjh2385225@163.com
更新日期/Last Update: 2011-09-20