[1]冷弘,刘慧涛,臧玉翠,等.复方大青叶注射液对体外培养HTH9细胞CXCR4启动子活性的影响*[J].郑州大学学报(医学版),2011,(05):681-30.
 LENG Hong,LIU Huitao,ZANG Yucui,et al.Effect of compound Folium Isatidis injection on activity of CXCR4 promoters in HTH9 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):681-30.
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复方大青叶注射液对体外培养HTH9细胞CXCR4启动子活性的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
681-30
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Effect of compound Folium Isatidis injection on activity of CXCR4 promoters in HTH9 cells in vitro
作者:
冷弘1刘慧涛2臧玉翠3马云云4李敏3靳静4赵国强3#
1)洛阳职业技术学院免疫学与病原生物学教研室 洛阳 471000 2)南阳油田总医院消化肾病科 南阳473132 3)郑州大学基础医学院微生物学与免疫学教研室 郑州 450001 4)河南省职工医学院微生物学与免疫学教研室 郑州 451191
Author(s):
LENG Hong1 LIU Huitao2ZANG Yucui3 MA Yunyun4LI Min3JIN Jing4ZHAO Guoqiang3
1)Department of Immunology and Pathogen Biology,Luoyang Vocation & Technical College,Luoyang 471000 2)Department of Gastroenterology and Nephrology, Nanyang Oilfield General Hospital,Nanyang 473132 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University, Zhengzhou 450001 4)Department of Microbiology and Immunology, Henan Medical College for Staff and Workers, Zhengzhou 451191
关键词:
复方大青叶注射液CXCR4启动子艾滋病HTH9细胞
Keywords:
compound Folium Isatidis injectionsCXCR4 promotorAIDSHTH9 cells
分类号:
R285.5
摘要:
目的:探讨复方大青叶注射液对体外培养HTH9细胞中CXCR4启动子活性的作用。方法:克隆人CXCR4启动子序列,构建报告载体pGL4.17CXCR4;转染HTH9细胞株,G418筛选后分组,高、低剂量实验组分别用200、100 mg/L大青液注射液处理,设注射水对照组、空白对照组和未转染对照组;各组处理24 h后,进行荧光素酶活性检测。结果:成功构建含人CXCR4启动子序列的报告载体PGL4.17CXCR4;5组荧光素酶活性比较差异有统计学意义(F=135.510,P<0.001)。复方大青叶注射液高剂量组与低剂量组荧光素酶活性均明显低于对照组(P<0.01)。结论:复方大青叶注射液能够有效抑制体外培养HTH9细胞中CXCR4启动子活性。
Abstract:
Aim:To investigate the effect of the compound Folium Isatidis injection on the activity of CXCR4 promoters in HTH9 cells cultivated in vitro.Methods:The sequence of CXCR4 promoter gene was cloned,and inserted into the reporter vector pGL4.17 to construct luciferase reporter vector (pGL4.17CXCR4),the recombinant plasmids were transfected into HTH9 cells.Screened by G418, the lasting transfected cells were allocated into 4 groups: high dose group and low dose group (treated with 200,100 mg/L compound Folium Isaticlis injection for 24 h,respectively), injection water control group,blank control group,and untransfection group. The luciferase activity was evaluated.Results:The results of restrict double digestions and DNA sequencing proved that pGL4.17CXCR4 was constructed successfully. The level of luciferase activity among the 5 groups had significant differences (F=135.510,P<0.001).The levels of the compound Folium Isatidis injection groups, both high dose group and low dose group, were remarkably lower than those of control groups (P<0.01).Conclusion: The compound Folium isatidis injection could obviously restrain the activity of CXCR4 promoters in HTH9 cells in vitro.

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备注/Memo

备注/Memo:
*河南省医学科技攻关基金资助项目2008002002 #通讯作者,男,1965年10月生,教授,研究方向:肿瘤病因学,Email:zhaogq@zzu.edu.cn
更新日期/Last Update: 2011-09-20