[1]丁培杰,段长恩,张世杰,等.重组人生长激素对人巨噬细胞分泌IL1、IL6和TNFα的影响*[J].郑州大学学报(医学版),2011,(05):684-34.
 DING Peijie,DUAN Changen,ZHANG Shijie,et al.Effect of recombinant human growth hormone on production of IL1, IL6, and TNFα from human macrophages[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):684-34.
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重组人生长激素对人巨噬细胞分泌IL1、IL6和TNFα的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
684-34
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Effect of recombinant human growth hormone on production of IL1, IL6, and TNFα from human macrophages
作者:
丁培杰1段长恩2张世杰3赵国强4#
1)漯河医学高等专科学校免疫学教研室 漯河 462000 2)新乡医学院免疫学教研室 新乡 453003 3)郑州大学第一附属医院检验科 郑州 450052 4)郑州大学基础医学院微生物学与免疫学教研室 郑州 450001
Author(s):
DING Peijie1DUAN Chang’en2ZHANG Shijie3ZHAO Guoqiang4
1)Department of Immunology,Luohe Medical Academy,Luohe 462000 2)Department of Immunology,Xinxiang Medical College,Xinxiang 453003 3)Clinical Laboratory,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 4)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001
关键词:
生长激素IL1IL6TNFα巨噬细胞
Keywords:
growth hormone IL1IL6TNFα macrophage
分类号:
R392
摘要:
目的:观察重组人生长激素(rhGH)对人巨噬细胞分泌IL1、IL6和TNFα的影响。方法:以粒细胞巨噬细胞集落刺激因子体外诱导从人外周血中分离的单个核细胞,使其分化为巨噬细胞,并用流式细胞术鉴定;诱导获得细胞分为6组,分别用1 mg/L脂多糖和0、1、4、20及50 μg/L的rhGH培养4、8和12 h,采用ELISA法测定各组巨噬细胞上清液中IL1、IL6和TNFα的分泌情况。结果:诱导的细胞经鉴定为巨噬细胞。不同质量浓度的rhGH均可促进所诱导的巨噬细胞分泌IL1、IL6和TNFα;随培养时间的延长,各组巨噬细胞以上细胞因子的分泌量增加。结论:rhGH对人单核巨噬细胞分泌IL1、IL6和TNFα有促进作用,提示其可能参与免疫调节作用。
Abstract:
Aim:To investigate the effects of recombinant human growth hormone(rhGH) on the production of IL1, IL6, and TNFα from human macrophages.Methods:The mononuclear cells were obtained from human peripheral blood and induced to macrophages by granulocyte and monocyte colony stimulating factor,and identified by flow cytometry. Then the macrophages were allocated into 6 groups and stimulated by lipopolysaccharide and 0,1,4,20, and 50 μg/L rhGH, respectively. The production of IL1, IL6, and TNFα from macrophages were detected by ELISA.Results:The macrophages were obtained and identified. It was showed that rhGH could promote macrophages to secrete IL1, IL6, and TNFα, and the contents of IL1,IL6,and TNFα increased with culture time.Conclusion:rhGH could promote the production of IL1,IL6 and TNFα by macrophages, suggesting that it may be involved in immune regulation.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省教育厅自然科学研究计划基金资助项目教科外[2008]78号 2008C310003 #通讯作者,男,1965年10月生,博士,教授,研究方向:分子免疫学,Email:zhaogq@zzu.edu.cn
更新日期/Last Update: 2011-09-20