[1]李幸乐,许玉玲,黄学勇,等.河南省2010年肠道病毒EV71型分离株全基因组序列分析*[J].郑州大学学报(医学版),2011,(05):699-51.
 LI Xingle,XU Yuling,HUANG Xueyong,et al.Complete sequence analysis of Enterovirus 71 strain from a fatal case of handfootmouth disease during an epidemic of Henan province in 2010[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):699-51.
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河南省2010年肠道病毒EV71型分离株全基因组序列分析*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
699-51
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Complete sequence analysis of Enterovirus 71 strain from a fatal case of handfootmouth disease during an epidemic of Henan province in 2010
作者:
李幸乐许玉玲黄学勇陈豪敏许汴利#
河南省疾病预防控制中心传染病所 郑州 450016
Author(s):
LI Xingle XU Yuling HUANG XueyongCHEN HaominXU Bianli
Infectious Disease Institute, Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016
关键词:
手足口病肠道病毒71型测序同源性分析
Keywords:
handfootmouth diseaseEnterovirus 71sequencing homology analysis
分类号:
R183.4
摘要:
目的:了解2010年河南省流行的肠道病毒(EV)71型基因特征。方法:筛选2010年河南省分离的EV71型病毒株1株(EV71/Henan/DC/2010),进行全基因组序列测定和基因进化特性分析。结果:EV71/Henan/DC/2010与其他EV71病毒株相比,编码区无核苷酸的缺失或插入,5’UTR区和3’UTR区长度和序列有一定差异。核苷酸同源性比较显示,EV71/Henan/DC/2010与2008年北京暴发流行株同源性最高(99.2%),与安徽阜阳暴发流行株和河南省暴发流行株均具有很高的同源性(98.1%和99.1%);与EV71各亚型代表株的比较结果显示,除VP3区以外,EV71/Henan/DC/2010各区均与C4亚型代表株同源性最高;氨基酸同源性比较结果显示,EV71/Henan/DC/2010大多数核苷酸变异属无义突变,相应氨基酸并未随之发生改变。根据VP1区基因序列构建遗传进化分析树显示,EV71/Henan/DC/2010与C4亚型同属一个分支。结论:EV71/Henan/DC/2010与2008年北京EV71流行株亲缘关系最为接近,同属于C4亚型。与我省及我国既往流行毒株相比,未发生大的变异。
Abstract:
Aim: To investigate the genetic characteristics of Enterovirus 71(EV71) circulating strains of Henan province in 2010.Methods:The EV71 strain isolated from a fatal case of handfootmouth disease during an epidemic of Henan in 2010 was chosen.Its complete genome was sequenced and analyzed comparatively.Results: The results showed that EV71/Henan/DC/2010 had no insertion or deletion detected in the coding region but several insertions and deletions in 5’and 3’UTR. Phylogenetic analysis of EV71/Henan/DC/2010 and reference strains showed it had the highest nuclyeotide homology with 2008 Beijing epidemicstrain FJ606448(99.2%),and with Anhui epidemicstrain EU703813 and Henan epidemicstrain GU366191(98.1% and 99.1%). The majority nucleotide variations were nonsensemutation, and the corresponding amino acid did not change along with it. In all but VP3 regions,EV71/Henan/DC/2010 had the highest nucleotide homology with C4 subtype. It clustered with reference strains of C4 subtype in the phylogenetic tree.Conclusion: EV71/Henan/DC/2010 strain has the nearest genetic relationship with 2008 Beijing epidemicstrain FJ606448, both belong to the C4 subtype. EV71/Henan/DC/2010 strain has not remarkable variation.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省医学科技攻关基金资助重大项目201001015 #通讯作者,男,1958年3月生,硕士,主任医师,研究方向:流行病与寄生虫病,Email:xubl@hncdc.com.cn
更新日期/Last Update: 2011-09-20