[1]颜秉兴△,孟英红,孙迎军,等.多黏菌素B联合不同抗菌药物抗耐碳青霉烯肺炎克雷伯菌和大肠埃希菌的协同作用[J].郑州大学学报(医学版),2011,(05):714-72.
 YAN Bingxing,MENG Yinghong,SUN Yingjun,et al.Synergy of polymyxin B in combination with several antimicrobial agents in defencing carbapenems resistant Klebsiella pneumoniae and Escherichia coli[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):714-72.
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多黏菌素B联合不同抗菌药物抗耐碳青霉烯肺炎克雷伯菌和大肠埃希菌的协同作用
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
714-72
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Synergy of polymyxin B in combination with several antimicrobial agents in defencing carbapenems resistant Klebsiella pneumoniae and Escherichia coli
作者:
颜秉兴孟英红孙迎军李建军
濮阳市油田总医院检验科 濮阳 457001
Author(s):
YAN Bingxing MENG Yinghong SUN Yingjun LI Jianjun
Clinical Laboratory, Puyang City Oilfield General Hospital,Puyang 457001
关键词:
碳青霉烯耐药协同部分抑菌浓度
Keywords:
carbapenemresistancesynergy fractional inhibitory concentration
分类号:
R978.1
摘要:
目的:观察多黏菌素B与几种抗菌药物联合抗耐碳青霉烯肺炎克雷伯菌和大肠埃希菌的协同作用。方法:16株碳青霉烯类耐药菌中肺炎克雷伯菌11株,大肠埃希菌5株,观察多黏菌素B与利福平、强力霉素、头孢吡肟、亚胺培南、头孢曲松和庆大霉素对上述菌株的协同作用,通过棋盘微量稀释法确定每种抗菌药物的最小抑菌浓度(MIC)值,根据MIC计算部分抑菌浓度指数(FICI),FICI<0.5定义为有协同作用。结果:试验菌株对除多黏菌素B、利福平和强力霉素外的抗菌药物均耐药。对于所有试验菌株,PB与RIF或DOX在生理浓度均存在协同作用,联合后PB的MIC值至少降低3倍以上。与其他研究药物的联合未观察到协同作用。结论:多黏菌素B与利福平、强力霉素体外联合抵御耐碳青霉烯大肠埃希菌和肺炎克雷伯菌存在协同作用。
Abstract:
Aim:To observe the synergy of polymyxin B in combination with several antimicrobial agents in defencing carbapenems resistant Klebsiella pneumoniae and Escherichia coli.Methods:Sixteen carbapenems resistant strains including 11 strains of Klebsiella pneumoniae and 5 strains of Escherichia coli were collected.The synergy of polymyxin B in combination with rifampin, doxycycline, cefepime, imipenem,ceftriaxone,or gentamicin in defencing the abovementioned strains was observed. MIC of each antibiotic was determined by microdilution checkerboard tests,and the FIC index(FICI) was calculated by MIC.Synergy was defined as FICI<0.5.Results: All strains were resistant to all of the tested antimicrobials with the exception of polymyxin B, rifampin and doxycycline. Synergy was observed in the combination of polymyxin B with rifampin and doxycycline in defencing all of the tested isolates, resulting in at least 3fold decrease in MIC. No synergy was observed in other combinations studied at physiologic concentrations.Conclusion: Synergy exists in the combination of polymyxin B with rifampin and doxycycline in defencing carbapenems resistant Klebsiella pneumoniae and Escherichia coli.

参考文献/References:

[1]Taccone FS, RodriguezVillalobos H,De Backer D. Successful treatment of septic shock due to panresistant Acinetobacter baumannii using combined antimicrobial therapy including tigecycline[J]. Eur J Clin Microbiol Infect Dis,2006, 25(4):257
[2]Elemam A, Rahimian J, Mandell W. Infection with panresistant Klebsiella pneumoniae: a report of 2 cases and a brief review of the literature[J]. Clin Infect Dis, 2007,49(2):271
[3]叶应妩, 王毓三, 申子瑜. 全国临床检验操作规程[M]. 3版.南京:东南大学出版社,2006.
[4]Clinical and Laboratory Standards Institute/NCCLS. Performance standards for antimicrobial susceptibility testing: 20th ed. CLSI/NCCLS M100S20[S]. Clinical and Laboratory Standards Institute, 2010,Wayne, Pa.
[5]Zavascki AP,Goldani LZ,Li J,et al. Polymyxin B for the treatment of multidrugresistant pathogens: a critical review[J]. J Antimicrob Chemother, 2007, 60(6):1206
[6]Bratu S, Tolaney P, Karumudi U,et al. Carbapenemaseproducing Klebsiella pneumoniae in Brooklyn, NY: molecular epidemiology and in vitro activity of polymyxin B and other agents[J].J Antimicrob Chemother,2005, 56(1): 128
[7]Elemam A, Rahimian J, Doymaz M, et al. In vitro evaluation of antibiotic synergy for polymyxin Bresistant carbapenemaseproducing Klebsiella pneumoniae[J]. J Clin Microbiol, 2010,48(10):3558
[8]Wareham DW, Bean DC. In vitro activity of polymyxin B in combination with imipenem, rifampicin and azithromycin versus multidrug resistant strains of Acinetobacter baumannii producing OXA23 carbapenemases[J]. Ann Clin Microbiol Antimicrob, 2006, 5:10

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备注/Memo

备注/Memo:
△男,1966年1月生,本科,副主任技师,研究方向:细菌耐药,Email:bingxingy00123@126.com
更新日期/Last Update: 2011-09-20