[1]向莉△,李书剑,张杰文.美洲商陆抗病毒蛋白对人神经胶质瘤细胞U251细胞增殖和凋亡的影响[J].郑州大学学报(医学版),2011,(05):742.
 XIANG Li,LI Shujian,ZHANG Jiewen.Effects of recombinant pokeweed antiviral proteins from Phytolacca amercana on the proliferation and apoptosis of Human Gliomaous Cells U251[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):742.
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美洲商陆抗病毒蛋白对人神经胶质瘤细胞U251细胞增殖和凋亡的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
742
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Effects of recombinant pokeweed antiviral proteins from Phytolacca amercana on the proliferation and apoptosis of Human Gliomaous Cells U251
作者:
向莉 李书剑 张杰文
河南省人民医院神经内科 郑州 450003
Author(s):
XIANG LiLI ShujianZHANG Jiewen
Department of Neurology,Henan Provincial People's Hospital, Zhengzhou 450003
关键词:
流式细胞美洲商陆抗病毒蛋白凋亡
Keywords:
flow cytometrypokeweed antiviral proteinsapoptosis
分类号:
R739.4
摘要:
目的:观察美洲商陆抗病毒蛋白(PAP)对人神经胶质瘤细胞U251细胞增殖和凋亡的影响。方法:采用MTT法检测0、20、40、 60、80和100 mg/L PAP处理48 h以及40 mg/L PAP处理24、36 、48 和72 h对神经胶质瘤细胞U251细胞生长的影响;40 mg/L PAP处理U251细胞36 h后,采用荧光染色技术检测细胞凋亡,采用流式细胞仪检测U251细胞周期分布的影响;采用Northern blot和Western blot检测40 mg /L PAP 处理24、48、72和96 h对U251细胞周期调控蛋白FasL和Fas的影响。结果:0、20、40、 60、80和100 mg/L PAP处理48 h,U251细胞的增殖抑制率间的差异有统计学意义(F=284.560,P=0.007),40 mg/L PAP处理24、36、48 和72 h ,U251细胞的增殖抑制率间的差异有统计学意义(F=280.250,P=0.045)。PAP促进U251细胞凋亡(t=106.350,P=0.007),PAP改变细胞周期分布,使G0+G1期细胞比例增高,S期比例降低。PAP还可上调FasL蛋白表达,下调Fas蛋白表达。结论:PAP可改变细胞周期分布, 影响细胞周期调控蛋白表达,并可诱导U251细胞凋亡,从而抑制细胞增殖。
Abstract:
Aim: To study the effects of pokeweed antiviral protein(PAP) on cell proliferation and apoptosis on U251 cells and to elucidate its molecular mechanism.Methods:The proportion of the periodic tumor cells were altered by 0,20,40, 60,80, 100 mg/L PAP treated 48 h using MTT and SubG1 curves were displayed at 40 mg/L PAP treated 36 h by flow cytometry analysis. With fluorescence staining assay to detect the U251 apoptosis. The mRNAs and protein expression of the FasL and Fas treated at 40 mg/L PAP was examined by Northern blot and Western blot.Results:PAP significantly suppressed U251 cell proliferation by 0,20,40,60,80,100 mg/L PAP treated 48 h(F=284.560,P=0.007) or 40 ng/L PAP treated 24,36,48,72 h (F=28.250,P=0.045).The proportion of the periodic tumor cells were altered by PAP.PAP decreased the proportion of cells in S phase and increased the proportion of cells in G0/G1 and G2/M phases of the cell cycle.The mRNAs and protein expression of the FasL downregulated gradually as treated time increased and the mRNA and protein expression levels of Fas was upregulated gradually as treated time increased.Conclusion:PAP can induce apoptosis and alert cell cycle arrest of the Human Gliomaous Cells U251 cells.

参考文献/References:

[1]Sturm MB,Tyler PC,Evans GB,et al.Transition state analogues rescue ribosomes from saporinL1 ribosome inactivating protein[J]. Biochemistry,2009,48(41):9941
[2]Barbieri L,Polito L,Bolognesi A,et al.Ribosomeinactivating proteins in edible plants and purification and characterization of a new ribosomeinactivating protein from Cucurbita moschata[J].Biochim Biophys Acta,2006,1760(5):783
[3]Kozlov IuV,Sudarkina OIu,Kurmanova AG.Ribosomeinactivating lectins from plants[J].Mol Biol,2006,40(4):711
[4]Aron GM,Irvin JD.Inhibition of herpes simplex virus multiplication by the pokeweed antiviral protein[J]. Antimicrob Agents Chemother,1980,17(6):1032
[5]Olson M, Ramakrishana S, Anand R. Ribosomal inhibitory proteins from plants inhibits HIV1 replication in acutely infected peripheral blood mononuclear cells[J]. AIDS Res Hum Retroviruses, 1991, 7(12): 1025
[6]陈国菊,石丽,雷建军,等.中国商陆抗病毒蛋白真核表达载体的构建及在比赤酵母中的表达[J].园艺学报,2008,35(6):847
[7]陈定虎,王锡锋,李莉,等.美洲商陆抗病毒蛋白真核表达载体的构建及在毕赤酵母中的表达[J].农业生物技术学报,2003,11(2):183

备注/Memo

备注/Memo:
△女,1969年5月生,硕士,副主任医师,研究方向:分子生物学,Email:xiangliyiyuan@163.com
更新日期/Last Update: 2011-09-20