[1]陈汇,许冰#.内皮素受体拮抗剂对肝硬化大鼠转化生长因子β1和Ⅰ型胶原mRNA表达的影响[J].郑州大学学报(医学版),2011,(05):745.
 CHEN Hui,XU Bing.Effects of ET receptor antagonist on expression of TGFβ1 and collagen typeⅠmRNA in hepatic cirrhotic rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):745.
点击复制

内皮素受体拮抗剂对肝硬化大鼠转化生长因子β1和Ⅰ型胶原mRNA表达的影响
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
745
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Effects of ET receptor antagonist on expression of TGFβ1 and collagen typeⅠmRNA in hepatic cirrhotic rats
作者:
陈汇许冰#
郑州大学第一附属医院肝胆胰外科 郑州 450052
Author(s):
CHEN HuiXU Bing
Department of Hepatopancreatobiliary Surgery, the First Affiliated Hospital, Zhengzhou University,Zhengzhou 450052
关键词:
内皮素受体拮抗剂肝硬化Ⅰ型胶原转化生长因子β1大鼠
Keywords:
ET receptor antagonistcirrhosiscollagen typeⅠTGFβ1rat
分类号:
R657.3
摘要:
目的:探讨内皮素受体拮抗剂对肝硬化大鼠转化生长因子β1和Ⅰ型胶原mRNA表达的影响。方法:40只雄性SD大鼠,随机分成四氯化碳组、正常对照组、内皮素A受体拮抗剂治疗组、内皮素B受体拮抗剂治疗组和联合治疗组5组,每组8只。后3组在四氯化碳灌胃的基础上2次/d (间隔10 h)分别皮下注射BQ123(12.5 μg/kg)和BQ788(15 μg/kg)以及BQ123+BQ788(12.5 μg/kg+15 μg/kg)。取部分肝组织进行常规病理学检测,部分采用RTPCR测定大鼠肝组织转化生长因子β1和Ⅰ型胶原mRNA表达水平。结果:常规病理结果显示内皮素受体拮抗剂处理组肝脏炎症反应及纤维化明显减轻。5组大鼠肝组织转化生长因子β1 mRNA表达水平比较,差异无统计学意义(F=2.857,P=0.765);Ⅰ型胶原mRNA表达水平比较[(0.437±0.082)、(0.623±0.142)、(0.655±0.124)、(0.558±0.183)和(0.874±0.170)],差异有统计学意义(F=11.235,P=0.023)。结论:内皮素受体拮抗剂能有效抑制肝硬化大鼠Ⅰ型胶原mRNA的表达, 缓解肝脏炎症反应和肝纤维化。
Abstract:
Aim: To investigate the effects of ET receptor antagonist on the expression of collagen typeⅠand TGFβ1 mRNA in carbon tetrachlorideinduced cirrhosis rats.Methods:A total of 40 male SD rats were allocated into carbon tetrachloride group, normal group,endothelin A receptor antagonist group, endothelin B receptor antagonist group,and combined treatment group. The posterior 3 groups were injected with BQ123(12.5 μg/kg),BQ788(15 μg/kg),and BQ123+BQ788,respectively,besides carbon tetrachloride treatment.The expressions of collagen typeⅠand TGFβ1 mRNA were determined by RTPCR.And a specific portion of liver tissue in every group was took for routine pathology testing.Results:The expression of TGFβ1 mRNA in 5 groups had no significant difference(F=2.857,P=0.765),but that of collagen typeⅠmRNA in 5 groups[(0.437±0.082),(0.623±0.142), (0.655±0.124), (0.558±0.183), and (0.874±0.170)] had significant differences (F=11.235,P=0.023).Microscopic image showed that inflammatory reaction decreased in the cirrhotic rats with injection of both ET receptor antagonists or combination administration.Conclusion: Endothelin receptor antagonists could inhibit the expression of hepatic collagen typeⅠin cirrhotic rats effectively,and reduce inflammation reaction and liver fibrosis.

参考文献/References:

[1]Feng HQ, Weymouth ND, Rockey DC. Endothelin antagonism in portal hypertensive mice: implications for endothelin receptorspecific signaling in liver disease[J]. Am J Physiol Gastrointest Liver Physiol, 2009,297(1):G27
[2]Gressner AM, Weiskirchen R. Modern pathogenetic concepts of liver fibrosis suggest stellate cells and TGFbeta as major players and therapeutic targets[J]. J Cell Mol Med, 2006,10(1):76
[3]Khimji AK, Shao R, Rockey DC. Divergent transforming growth factorβ signaling in hepatic stellate cells after liver injury: functional effects on ECE1 regulation[J].Am J Pathol,2008,173(3):716
[4]许冰,谢继辉.内皮素受体拮抗剂对肝硬变门静脉高压症大鼠门静脉压及ET1 mRNA表达的影响[J].郑州大学学报:医学版, 2004,39(5):829
[5]Atzori L, Poli G, Perraa A. Hepatic stellate cell: a star cell in the liver[J]. Int J Biochem Cell Biol,2009,41(8/9):1639
[6]Kordes C,Sawitza I,Hussinger D.Hepatic and pancreatic stellate cells in focus[J].Biol Chem,2009,390(10):1003
[7]Sysa P, Potter JJ, Liu X, et al. Transforming growth factorbeta1 upregulation of human alpha(Ⅰ) collagen is mediated by Sp1 and Smad2 transacting factors[J]. DNA Cell Biol, 2009,28(9):425
[8]Wynn TA,Barron L.Macrophages:master regulators of inflammation and fibrosis[J].Semin Liver Dis,2010,30(3):245
[9]Wynn TA. Cellular and molecular mechanisms of fibrosis[J]. J Pathol, 2008,214(2):199

相似文献/References:

[1]管生,李明省,马南,等.超选择性经肝动脉化疗栓塞术联合射频消融治疗严重肝硬化合并小肝癌36例[J].郑州大学学报(医学版),2010,(04):666.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(05):666.
[2]邹黎),毛建娜),郭长青).肝源性糖尿病80例临床分析[J].郑州大学学报(医学版),2010,(04):687.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(05):687.
[3]苏英),郭长青),高鹏),等.HCV所致慢性肝病中肝源性糖尿病的发生率与血糖水平[J].郑州大学学报(医学版),2012,(03):422.
[4]李焱,孙长宇#,周言.非侵入性指标诊断肝硬化食管静脉曲张的价值*[J].郑州大学学报(医学版),2013,(04):505.
 LI Yan,SUN Changyu,ZHOU Yan.Value of noninvasive parameters in dignosis of esophageal varices for patients with liver cirrhosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(05):505.
[5]梁丽),顾福嘉),黄国美)#,等.脐血干细胞移植治疗失代偿期肝硬化效果观察*[J].郑州大学学报(医学版),2014,(04):552.
 LIANG Li,GU Fujia,HUANG Guomei,et al.Efficacy of umbilical cord blood stem cell transplantation for decompensated cirrhosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2014,(05):552.
[6]张香梅△,杨桂林,乐晓华,等.肝细胞癌合并乙型肝炎肝硬化患者癌组织中长链非编码RNA表达谱分析*[J].郑州大学学报(医学版),2015,(02):194.
 ZHANG Xiangmei,YANG Guilin,LE Xiaohua,et al.Expression profiles of long noncoding RNA in HCC patients suffering hepatitis B liver cirrhosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(05):194.
[7]易佳音,张淑凤,冯慧芬,等.血清胆汁酸谱系测定在肝硬化诊断中的应用价值[J].郑州大学学报(医学版),2019,(06):922.[doi:10.13705/j.issn.1671-6825.2019.01.008]
 YI Jiayin,ZHANG Shufeng,FENG Huifen,et al.Application value of serum bile acid lineage measurement in diagnosis of cirrhosis[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2019,(05):922.[doi:10.13705/j.issn.1671-6825.2019.01.008]

备注/Memo

备注/Memo:
#通讯作者,男,1968年12月生,博士,副教授,研究方向:肝硬化门脉高压和布加综合征,Email:bingxumd@126.com
更新日期/Last Update: 2011-09-20