[1]赵彦坡,秦洁,孙石磊#.美金刚对大鼠脑缺血再灌注后Caspase3表达及丙二醛含量的影响[J].郑州大学学报(医学版),2011,(05):748.
 ZHAO Yanpo,QIN Jie,SUN Shilei.Effects of memantine on expression of Caspase3 and MDA in rats after ischemiareperfusion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):748.
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美金刚对大鼠脑缺血再灌注后Caspase3表达及丙二醛含量的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
748
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Effects of memantine on expression of Caspase3 and MDA in rats after ischemiareperfusion
作者:
赵彦坡秦洁孙石磊#
郑州大学第一附属医院神经内科 郑州 450052
Author(s):
ZHAO YanpoQIN JieSUN Shilei
Department of Neurology, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052
关键词:
迟发损伤美金刚脑缺血Caspase3丙二醛大鼠
Keywords:
injury of delaymemantinecerebral ischemiaCaspase3MDArat
分类号:
R743
摘要:
目的:观察非竞争性NMDA受体拮抗剂美金刚对大鼠脑缺血再灌注后迟发性脑损伤的抑制作用。方法:雄性Wistar大鼠135只,随机分为假手术组、模型组及干预组,采用线栓法建立大鼠大脑中动脉闭塞(MCAO)局灶性脑缺血再灌注模型,干预组用美金刚溶液灌胃,HE染色观察神经元形态变化,免疫组织化学染色检测神经元Caspase3的表达变化,分光光度计检测其Caspase3、MDA含量变化。结果:再灌注后12、24和48 h假手术组、模型组及干预组的Caspase3阳性区平均积分光密度、Caspase3酶活性和MDA含量比较,差异有统计学意义(F=63.900、246.589、437.277,41.151、82.116、106.407,387.615、209.846、475.285,P均<0.001)。干预组Caspase3表达及MDA含量较模型组降低,而高于假手术组,差异有统计学意义(P<0.05)。结论:美金刚可下调大鼠脑缺血再灌注后Caspase3表达及MDA含量,阻止迟发性脑损伤而发挥神经保护作用。
Abstract:
Aim: To investigate the inhibition of NMDA receptor antagonist memantine on the cerebral ischemic injury of delay in rats.Methods:One hundred and thirtyfive male Wistar rats were randomly divided into 3 groups:shamoperation group,model group,and intervention group. The models of cerebral ischemiareperfusion injury rats were established by the middle cerebral artery occlusion(MCAO).The rats of intervention group were treated with memantine. The changes of cell morphology and the expression of Caspase3 in cerebral cortex neurons were observed by HE staining and immunohistochemistry technique,respectively.The contents of Caspase3 and MDA were detected by spectrophotometer.Results:The expression of Caspase3,Caspase3 enzyme activity and the level of MDA among the 3 groups at 12,24,and 48 h were different(F=63.900, 246.589, 437.277;41.151, 82.116, 106.407;387.615, 209.846,475.285,P<0.001). The expression levels of Caspase3 and MDA in intervention group were lower than those of model group but higher than those of shamoperation group(P<0.05).Conclusion:NMDA receptor antagonist could suppress the expression of Caspase3 and MDA in ischemiareperfusion brain and inhibit injury of delay in rats to play a neuroprotective role.

参考文献/References:

[1]Rogalewski A, Schneider A, Ringelstein EB,et al. Toward a multimodal neuroprotective treatment of stroke[J].Stroke, 2006,37(4):1129
[2]Culmsee C, Junker V, Kremers W, et al.Combination therapy in ischemic stroke: synergistic neuroprotective effects of memantine and clenbuterol[J].Stroke, 2004,35(5):1197
[3]Dogan A,Eras MA,Rao VL,et al.Protective effects of memantine against ischemiareperfusion injury in spontaneously hypertensive rats[J]. Acta Neurochir (Wien),1999,141(10):1107
[4]Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats[J].Stroke, 1989,20(1):84
[5]Krivonos OV, Amosova NA,Smolentseva IG. Use of the glutamate NMDA receptor antagonist PKMerz in acute stroke[J].Neurosci Behav Physiol,2010,40(5):529
[6]Monaghan DT,Cotman CW. Distribution of NmethylDaspartatesensitive L
[3H]glutamatebinding sites in rat brain[J].J Neurosci, 1985,5(11):2909
[7]JantasSkotniczna D,Kajta M, Lasoń W, et al.Memantine attenuates staurosporineinduced activation of caspase3 and LDH release in mouse primary neuronal cultures [J].Brain Res,2006,1069(1):145
[8]Zhang G, Dong Y, Zhang B, et al. Isofluraneinduced caspase3 activation is dependent on cytosolic calcium and can be attenuated by memantine[J]. Neuroscience,2008,28(17):4551
[9]Ozsüer H, Gorgülü A, Kiris T, et al.The effects of memantine on lipid peroxidation following closedhead trauma in rats [J]. Neurosurg Rev,2005,28(2):143
[10]Qiu W, Wei R, Zhang C, et al.Aglycine sitespecific NMDA receptor antagonist protects retina ganglion cells from ischemic injury by modulating apoptotic cascades[J].J Cell Physiol,2010,223(3):819
[11]Hu NW, Klyubin I, Anwyl R,et al.GluN2B subunitcontaining NMDA receptor antagonists prevent Abetamediated synaptic plasticity disruption in vivo[J].Proc Natl Acad Sci USA,2009,106(48):20504
[12]Szegedi V, Juhász G, Parsons CG, et al.In vivo evidence for functional NMDA receptor blockade by memantine in rat hippocampal neurons[J].J Neural Transm,2010,117(10):1189

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备注/Memo

备注/Memo:
#通讯作者,女,1969年9月生,博士,副主任医师,研究方向:脑血管病,Email:sunshilei@hotmail.com
更新日期/Last Update: 2011-09-20