[1]亢勤△.慢性乙醇中毒小鼠海马Caspase3的表达[J].郑州大学学报(医学版),2011,(05):754.
 KANG Qin.Expression of Caspase3 in hippocampus of chronic alcoholic dementia mice[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(05):754.
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慢性乙醇中毒小鼠海马Caspase3的表达
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年05期
页码:
754
栏目:
论著
出版日期:
2011-09-20

文章信息/Info

Title:
Expression of Caspase3 in hippocampus of chronic alcoholic dementia mice
作者:
亢勤
河南省建筑医院内科 郑州 450001
Author(s):
KANG Qin
Department of Internal Medicine,Building Hospital of Henan Province,Zhengzhou 450001
关键词:
慢性乙醇中毒Caspase3小鼠
Keywords:
chronic alcoholic dementiaCaspase3mouse
分类号:
R595.6
摘要:
目的:探讨慢性乙醇中毒小鼠海马Caspase3的表达。方法:小鼠随机分为3组,正常对照组,高、低剂量乙醇组,每组6只,雌雄各半。高、低剂量组分别灌胃2.4和4.8 mL/d无水乙醇,以建立慢性酒精中毒小鼠模型;正常对照组灌胃等量生理盐水。采用Morris型水迷宫实验检测小鼠学习记忆功能;出现学习记忆功能障碍2周后免疫组织化学方法检测小鼠海马激活型Caspase3的表达,Western Blotting法检测Caspase3蛋白表达。结果:高剂量组(14±2) d后出现学习记忆功能障碍,早于低剂量组的(28±3) d(t=9.511,P<0.001)。3组小鼠大脑海马区激活型Caspase3和Caspase3蛋白表达差异有统计学意义(F=22.110、23.060,P<0.001),高剂量组表达高于低剂量组(P<0.05)。结论:慢性乙醇中毒引起脑功能退化可能与脑细胞凋亡有关。
Abstract:
Aim:To study the expression of Caspase3 in hippocampus of chronic alcoholic dementia mice.Methods:Mice were allocated in 3 groups:normal control group, highdose and lowdose alcohol group,and 6 in each group.The mice in highdose and lowdose group were given intragastricly 4.8 and 2.4 mL/d absolute ethyl alcohol to establish chronic alcoholic dementia model. The learningmemory function was detected by Morris water maze experiment,and 2 weeks later,immunohistochemical staining and Westernblotting were used to detect the expressions of activated Caspase3 and Caspase3 protein in hippocampus.Results:Mice in highdose group showed learningmemory dysfunction after (14±2) d,earlier than that (28±3) d of lowdose group(t=9.511,P<0.001).There were significant differences in activated Caspase3 protein and Caspase3 protein in hippocampus among the three groups(F=22.110,23.060,P<0.001),and the expressions of activated Caspase3 protein and Caspase3 protein in hippocampus of highdose group were higher than those of lowdose group(P<0.05).Conclusion:Learningmemory dysfunction induced by chronic alcoholic dementia may be related with cell apoptosis through caspasedependent pathway.

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备注/Memo

备注/Memo:
△男,1968年8月生,本科,主治医师,研究方向:脑血管疾病
更新日期/Last Update: 2011-09-20