[1]李靓,石科,李俊平,等.杜氏盐藻鞭毛相关蛋白cDNA片段的克隆及在鞭毛重吸收过程中的表达*[J].郑州大学学报(医学版),2011,(06):821.
 LI Liang,SHI Ke,LI Junping,et al. Cloning and expression of flagella associated protein cDNA fragment from Dunaliella salina and its expression during flagellar reabsorption[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):821.
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杜氏盐藻鞭毛相关蛋白cDNA片段的克隆及在鞭毛重吸收过程中的表达*()
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
821
栏目:
专题研究
出版日期:
2011-11-20

文章信息/Info

Title:
 Cloning and expression of flagella associated protein cDNA fragment from Dunaliella salina and its expression during flagellar reabsorption
作者:
李靓石科李俊平柴丹丹薛乐勋#
 郑州大学生物工程系细胞生物学研究室 郑州 450001
Author(s):
 LI LiangSHI KeLI JunpingCHAI DandanXUE Lexun
 Laboratory for Cell Biology, Department of Bioengineering, Zhengzhou University,Zhengzhou 450001
关键词:
 杜氏盐藻鞭毛相关蛋白秋水仙碱
分类号:
Q781
文献标志码:
A
摘要:
 目的:克隆杜氏盐藻鞭毛相关蛋白(FAP)的cDNA片段并探讨其功能。方法:分析莱茵衣藻等生物的FAP同源蛋白的氨基酸序列保守区域,设计简并引物。提取盐藻总RNA进行RTPCR。根据得到的序列设计3’RACE引物,巢式PCR扩增该cDNA的3’端序列。秋水仙碱处理对数生长期的盐藻细胞,使细胞停留在分裂中期,并诱导鞭毛缩短,半定量PCR检测FAP基因的表达情况。结果:RTPCR和3’RACE分别得到长1 535 bp和782 bp的cDNA片段,拼接后总长2 141 bp,编码541个氨基酸。序列比对发现与莱茵衣藻的FAP(88%)、团藻CDC48(89%)氨基酸序列均有较高的同源性。秋水仙碱处理后盐藻细胞FAP mRNA表达量高于未处理对照组(F组间=43.192,P<0.001;F时间=2.659,P=0.043;F交互=594.419,P<0.001)。结论:成功获得杜氏盐藻FAP的cDNA片段,该基因在秋水仙碱诱导的鞭毛重吸收过程中表达量增高。

参考文献/References:

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备注/Memo

备注/Memo:
 *国家自然科学基金资助项目30700014;科技部国际科技合作基金资助项目2007DFA01240  #通讯作者,男,1944年2月生,教授,博士研究生导师,研究方向:肿瘤标志物与基因工程,Email:xuelx@zzu.edu.cn
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