[1]李俊平,刘岷,毛丽红,等.杜氏盐藻FLA8基因全长的克隆及鉴定*[J].郑州大学学报(医学版),2011,(06):828.
 LI Junping,LIU Min,MAO Lihong,et al.Cloning and identification of the fulllength cDNA sequence of FLA8 gene from Dunaliella salina[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):828.
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杜氏盐藻FLA8基因全长的克隆及鉴定*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
828
栏目:
专题研究
出版日期:
2011-11-20

文章信息/Info

Title:
Cloning and identification of the fulllength cDNA sequence of FLA8 gene from Dunaliella salina
作者:
李俊平刘岷毛丽红石科李靓许尧薛乐勋#
郑州大学生物工程系细胞生物学研究室 郑州 450003
Author(s):
LI Junping LIU Min MAO LihongSHI KeLI LiangXU Yao XUE Lexun
Laboratory for Cell Biology, Department of Bioengineering, Zhengzhou University,Zhengzhou 450003
关键词:
杜氏盐藻FLA8基因驱动蛋白2
Keywords:
Dunaliella salinaFLA8 kinesin2
分类号:
Q781
摘要:
目的:获得 FLA8 基因cDNA序列。方法:根据衣藻、小球藻等驱动蛋白2亚基的氨基酸高度保守序列GYNGTIF、NEDPKDA设计一对简并引物,采用 RTPCR 及 3’RACE 和5’RACE的方法扩增杜氏盐藻FLA8基因的全长。结果:克隆得到的杜氏盐藻FLA8基因cDNA全长序列为2 612 bp,序列分析显示其包括90 bp的5’UTR、167 bp的3’UTR以及2 355 bp的开放阅读框,其编码784个氨基酸残基,蛋白质的理论等电点为6.65,相对分子质量为85 097。氨基酸序列同源性分析显示其与其他物种有较高的同源性:团藻(98%)、衣藻(99%)、小球藻(92%)。结论:得到杜氏盐藻驱动蛋白2亚基FLA8的基因全长。
Abstract:
Aim:To get the fulllength cDNA sequence of FLA8 gene from Dunaliella salina (D. salina).Methods:A pair of degenerated primers was designed according to conserved homologous amino acid sequences of GYNGTIF and NEDPKDA of kinesin2 motor subunit of FLA8 from microalgae and other organisms. The fulllength cDNA of FLA8 gene was obtained by RTPCR, 3’and 5’RACE technology.Results:The results showed that the fulllength cDNA sequence of the cloned FLA8 gene was 2 612 bp,possessing a 90 bp 5’UTR, a 167 bp 3’UTR and a 2 355 bp open reading frame encoding 784 amino acids. The theoretical pI value of FLA8 was 6.65 and the relative molecular weight was 85 097.Amino acid sequence homologous analysis indicated that FLA8 of D. salina shared high homology with other organisms, such as Volvox carteri f. nagariensis(98%),Chlamydomonas reinhardtii(99%), Micromonas(92%).Conclusion:The fulllength cDNA sequence of FLA8 gene from D. salina has been cloned.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30700014;科技部国际科技合作基金资助项目2007DFA01240 #通讯作者,男,1944年2月生,教授,博士研究生导师,研究方向:肿瘤标志物与基因工程,Email:xuelx@zzu.edu.cn
更新日期/Last Update: 1900-01-01