[1]罗萍)△,杜松),段红艳),等.普罗布考对过氧化氢诱导的大鼠心肌细胞凋亡及氧化应激水平的影响*[J].郑州大学学报(医学版),2011,(06):846.
 LUO Ping),DU Song),DUAN Hongyan),et al.Inhibition of probucol on cultured neonatal rat cardiomyocyte apoptosis induced by hydrogen peroxide[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):846.
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普罗布考对过氧化氢诱导的大鼠心肌细胞凋亡及氧化应激水平的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
846
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Inhibition of probucol on cultured neonatal rat cardiomyocyte apoptosis induced by hydrogen peroxide
作者:
罗萍1)杜松1)段红艳1)李海霞2)
1)河南省人民医院心内科 郑州 450003 2)河南省人民医院检验科 郑州450003
Author(s):
LUO Ping1)DU Song1)DUAN Hongyan1)LI Haixia2)
1)Department of Cardiology,Henan Provincial People’s Hospital,Zhengzhou 450003 2)Department of Clinical Laboratory,Henan Provincial People’s Hospital,Zhengzhou 450003
关键词:
普罗布考过氧化氢丙二醛超氧化物歧化酶BaxBcl2凋亡大鼠心肌细胞
Keywords:
probucolhydrogen peroxidemalondialdehydesuperoxide dismutaseBaxBcl2apoptosisratcardiomyocyte
分类号:
R972
摘要:
目的:探讨普罗布考对过氧化氢(H2O2)诱导的心肌细胞凋亡的影响,探讨其可能的机制。方法:培养的新生大鼠心肌细胞随机分组,分别给予0、1、10和100 μmol/L普罗布考预处理6 h后,再给予0.2 mmol/L H2O2继续孵育6 h。采用流式细胞仪检测细胞凋亡率,RTPCR法检测细胞Bcl2及Bax mRNA的表达,黄嘌呤氧化酶法测定细胞内超氧化物歧化酶(SOD)活性,硫代巴比妥酸法检测细胞内丙二醛(MDA)含量。结果:普罗布考剂量依赖性地抑制H2O2诱导的心肌细胞凋亡,降低心肌细胞Bax mRNA表达,升高Bcl2 mRNA表达,升高心肌细胞SOD活性,降低MDA含量。结论:普罗布考可抑制H2O2诱导的乳鼠心肌细胞凋亡,其作用机制与改善心肌细胞氧化应激水平有关。
Abstract:
Aim:To observe the effect of probucol on cardiomyocyte apoptosis induced by hydrogen peroxide(H2O2) and to explore its possible mechanisms.Methods:Cultured neonatal rat cardiomyocytes were randomly divided into 4 groups,and were given 0,1,10,and 100 μmol/L probucol pretreatment for 6 h,respectively,and then induced by 0.2 mmol/L H2O2 for 6 h.Apoptosis cells were assessed by flow cytometry. The mRNA expression of Bcl2 and Bax in cardiomyocytes was determined by RTPCR. The superoxide dismutase(SOD) activity and malondialdehyde(MDA) contents in cells were tested by xanthine oxidase method and color reaction of thiobarbituric acid method, respectively.Results:Probucol decreased cardiomyocyte apoptosis induced by H2O2 and Bax mRNA expression,increased Bcl2 mRNA expression,increased SOD activity and decreased the MDA content significantly. All these effects were in a dosedependent manner.Conclusion:Probucol could inhibit cardiomyocyte apoptosis induced by H2O2 partly by reducing the oxidative stress level.

参考文献/References:

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备注/Memo

备注/Memo:
*河南省科技厅基础与前沿项目资助课题112300410049 △女,1977年9月生,硕士,主治医师,研究方向:冠心病的基础与临床,Email:TJXLLP@163.com
更新日期/Last Update: 1900-01-01