[1]李笑竹,赵贵森#,岳阳阳,等.Hela细胞雄激素受体基因外显子1的甲基化检测*[J].郑州大学学报(医学版),2011,(06):856.
 LI Xiaozhu,ZHAO Guisen,YUE Yangyang,et al.Methylation analysis of androgen receptor exon1 in Hela cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):856.
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Hela细胞雄激素受体基因外显子1的甲基化检测*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
856
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Methylation analysis of androgen receptor exon1 in Hela cells
作者:
李笑竹赵贵森#岳阳阳翟仙敦艾红伟薛小琦
河南科技大学法医学院法医物证学教研室 洛阳 471003
Author(s):
LI Xiaozhu ZHAO Guisen YUE Yangyang ZHAI Xiandun AI Hongwei XUE Xiaoqi
Department of Forensic Biological Evidence,School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003
关键词:
雄激素受体DNA甲基化Hela细胞
Keywords:
androgen receptor DNA methylation Hela cell line
分类号:
R737.3
摘要:
目的:检测Hela细胞雄激素受体(AR)基因外显子1的甲基化水平。方法:用亚硫酸氢盐克隆测序法和联合亚硫酸氢盐的限制酶法(COBRA)检测不同来源Hela细胞AR基因外显子1的甲基化水平,BiQ Analyzer软件分析测序结果。结果:在非CpG胞嘧啶转化率>98%的前提下,Hela细胞AR基因外显子1片段的总甲基化率≥96.2%,除第8个CpG位点外,其他CpG位点均为完全甲基化或高甲基化。COBRA法与测序法结果一致。结论:DNA甲基化可能是Hela细胞AR基因失活的分子机制。
Abstract:
Aim:To investigate the methylation level of the androgen receptor(AR) exon1 in Hela cells.Methods:Methylation analysis of the AR exon1 was performed by bisulfite genomic sequencing(BGS) and combined bisulfite restriction analysis(COBRA). The sequencing data were analyzed by BiQ Analyzer.Results:At relatively high conversion rate (>98%), the methylation ratio of the AR exon1 was still more than 96.2%. Except for the eighth CpG, all other CpGs under investigations were fully or highly methylated. The results of COBRA were in accordance with that of BGS.Conclusion: DNA methylation may involve in the inactivation of AR gene in Hela cells.

参考文献/References:

[1]Benjamin CL, Jenster G, Piedrahita JA. Use of artificial androgen receptor coactivators to alter myoblast proliferation[J]. J Steroid Biochem Mol Biol,2004,91(3):111 [2]Liu L, Zhang J, Bates S, et al. A methylation profile of in vitro immortalized human cell lines[J]. Int J Oncol,2005,26(1):275 [3]Olek A, Oswald J, Walter J. A modified and improved method for bisulphite based cytosine methylation analysis[J]. Nucleic Acids Res,1996,24(24):5064 [4]Li LC, Dahiya R. MethPrimer: designing primers for methylation PCRs[J]. Bioinformatics,2002,18(11):1427 [5]Bock C,Reither S,Mikeska T,et al.BiQ Analyzer: visualization and quality control for DNA methylation data from bisulfite sequencing[J].Bioinformatics,2005,21(21):4067 [6]Grunau C, Clark SJ, Rosenthal A. Bisulfite genomic sequencing: systematic investigation of critical experimental parameters[J]. Nucleic Acids Res,2001,29(13):E65 [7]Sasaki M, Oh BR, Dharia A, et al. Inactivation of the human androgen receptor gene is associated with CpG hypermethylation in uterine endometrial cancer[J]. Mol Carcinog,2000,29(2):59 [8]Li LC, Carroll PR, Dahiya R. Epigenetic changes in prostate cancer: implication for diagnosis and treatment[J]. J Natl Cancer Inst,2005,97(2):103 [9]Leader JE,Wang C,Fu M,et al.Epigenetic regulation of nuclear steroid receptors[J].Biochem Pharmacol,2006,72(11):1589

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备注/Memo

备注/Memo:
*河南省重点科技攻关基金资助项目082102310097;河南科技大学科研基金资助项目2007QN013 #通讯作者,男,1972年12月生,博士,副教授,研究方向:分子遗传学,Email:lyfy@mail.haust.edu.cn
更新日期/Last Update: 1900-01-01