[1]刘瑜,王娜,冯斐斐,等.非小细胞肺癌组织中CD68与cJun蛋白的表达*[J].郑州大学学报(医学版),2011,(06):861.
 LIU Yu,WANG Na,FENG Feifei,et al.Expressions of CD68 and cJun proteins in nonsmall cell lung cancer tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):861.
点击复制

非小细胞肺癌组织中CD68与cJun蛋白的表达*
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
861
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Expressions of CD68 and cJun proteins in nonsmall cell lung cancer tissue
作者:
刘瑜王娜冯斐斐燕贞王威张少锋秦丽娟吴逸明#
郑州大学公共卫生学院劳动卫生学教研室 郑州 450001
Author(s):
LIU YuWANG NaFENG FeifeiYAN ZhenWANG WeiZHANG ShaofengQIN Lijuan WU Yiming
Department of Occupational Health,college of Public Health, Zhengzhou University, Zhengzhou 450001
关键词:
CD68巨噬细胞cJun非小细胞肺癌慢性炎症
Keywords:
CD68 macrophagecJun nonsmall cell lung cancerchronic inflammation
分类号:
R734.2
摘要:
目的:探讨非小细胞肺癌(NSCLC)组织中CD68和cJun蛋白的表达与肺癌发生发展的关系及其意义。方法:采用免疫组化SP法检测67例人NSCLC标本(每个标本取癌、癌旁和正常组织)中CD68和cJun蛋白的表达,应用Spearman秩相关检验CD68阳性细胞数与cJun蛋白表达的相关性。结果:肺癌、癌旁及正常肺组织中CD68阳性细胞数依次为11.8±1.4、97.5±2.5和52.1±1.9,差异有统计学意义(F=52 334.934,P<0.001);cJun蛋白的平均光密度值依次为0.25±0.03、0.23±0.02和0.20±0.01,差异有统计学意义(F=91.280, P<0.001)。癌旁组织中CD68阳性细胞数与年龄、性别、病理类型、分化程度、吸烟无关(P>0.05),与TNM分期(F=158.273, P<0.001)、淋巴结转移(F=84.985, P<0.001)、是否侵及胸膜(F=16.422, P<0.001)有关。癌旁组织中cJun蛋白的表达与年龄、性别、病理类型、吸烟无关(P>0.05),与TNM分期(F=19.319, P<0.001)、分化程度(F=33.918,P<0.001)、淋巴结转移(t=12.155,P<0.001)、是否侵及胸膜(F=8.318, P=0.005)有关。在癌旁组织中CD68阳性细胞数与cJun蛋白的表达呈正相关(rs=0.519, P<0.001)。结论:巨噬细胞主要浸润在癌旁组织中,可能通过激活蛋白1促进NSCLC的发展转移。
Abstract:
Aim:To explore the expressions of CD68 and cJun proteins in nonsmall cell lung cancer(NSCLC),and their relationship with the development of lung cancer.Methods:Immunohistochemical SP method was used to detect the expression of CD68 and cJun proteins in 67 cases of NSCLC samples including cancer tissues,adjacent tissues and normal tissues,and Spearman rank correlation analysis was used to investigate the correlation of CD68+ cell number with the expression of cJun protein.Results:CD68+ cell number in cancer tissues,adjacent tissues and normal tissues was 11.8±1.4, 97.5±2.5, 52.1±1.9,respectively,and there was statistical significance (F=52 334.934,P<0.001). The mean optical density of cJun protein in cancer tissues,adjacent tissues and normal tissues was 0.25±0.03, 0.23±0.02, 0.20±0.01, and there was statistical significance(F=91.280,P<0.001).The CD68+ cell number in adjacent tissues was correlated with the TNM stage(F=158.273, P<0.001), lymph node metastasis(F=84.985, P<0.001) and involvement of pleura(F=16.422,P<0.001).The expression of cJun in adjacent tissues was correlated with TNM stage(F=19.319,P<0.001),the differentiation of lung cancer(F=33.918,P<0.001),lymph node metastasis(F=12.155,P<0.001) and involvement of pleura(F=8.318,P=0.005).In adjacent tissues CD68+ cell number was positiuely correlated with the expression of cJun protein(rs=0.519, P<0.001).Conclusion:Macrophages mainly infiltrated in tumoradjacent tissues, and they may promote the development and metastasis of NSCLC by activating AP1.

参考文献/References:

[1]Ono M. Molecular links between tumor angiogenesis and inflammation: inflammatory stimuli of macrophages and cancer cells as targets for therapeutic strategy[J]. Cancer Sci,2008,99(8):1501 [2]Balkwill F, Mantovani A. Inflammation and cancer: back to virchow?[J]. Lancet,2001,357(9255):539 [3]Reddy SPM, Mossman BT. Role and regulation of activator protein1 in toxicantinduced responses of the lung[J]. Am J Physiol Lung Cell Mol Physiol,2002,283(6):L1161 [4]Lewis CE, Pollard JW. Distinct role of macrophages in different tumor microenvironments[J]. Cancer Res,2006,66(2):605 [5]Sica A, Larghi P, Mancino A, et al. Macrophage polarization in tumour progression[J]. Semin Cancer Biol,2008,18(5):349 [6]Leek RD, Lewis CE, Whitehouse R, et al. Association of macrophage infiltration with angiogenesis and prognosis in invasive breast carcinoma[J]. Cancer Res,1996,56(20):4625 [7]Leek RD, Talks KL, Pezzella F, et al. Relation of hypoxiainducible factor2 alpha(HIF2 alpha) expression in tumorinfiltrative macrophages to tumor angiogenesis and the oxidative thymidine phosphorylase pathway in human breast cancer[J]. Cancer Res,2002,62(5):1326 [8]Bingle L, Brown NJ, Lewis CE. The role of tumourassociated macrophages in tumour progression: implications for new anticancer therapies[J]. J Pathol,2002,196(3):254 [9]Mantovani A, Allavena P,Sica A,et al. Cancerrelated inflammation[J]. Nature,2008,454(7203):436 [10]Lin EY, Nguyen AV, Russell RG, et al. Colony stimulating factor 1 promotes progression of mammary tumors to malignancy[J]. J Exp Med,2001,193(6):727 [11]Vesely PW, Staber PB, Hoefler G, et al. Translational regulation mechanisms of AP1 proteins[J]. Mutat Res,2009,682(1):7 [12]Tichelaar JW, Yan Y,Tan Q,et al.A dominantnegative cjun mutant inhibits lung carcinogenesis in mice[J].Cancer Prev Res(Phila),2010,3(9):1148 [13]Hess J, Angel P, SchorppKistner M. AP1 subunits: quarrel and harmony among siblings[J]. J Cell Sci,2004,117(Pt25):5965 [14]Hagemann T, Wilson J, Kulbe H, et al. Macrophages induce invasiveness of epithelial cancer cells via NFκB and JNK[J]. J Immunol,2005,175(2):1197

相似文献/References:

[1]蒋翡翎)△,单保恩),姚敏),等.卡介苗蛋白提取物体外对小鼠腹腔巨噬细胞产生NO的影响[J].郑州大学学报(医学版),2010,(04):621.
 [J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(06):621.
[2]丁培杰,段长恩,张世杰,等.重组人生长激素对人巨噬细胞分泌IL1、IL6和TNFα的影响*[J].郑州大学学报(医学版),2011,(05):684.
 DING Peijie,DUAN Changen,ZHANG Shijie,et al.Effect of recombinant human growth hormone on production of IL1, IL6, and TNFα from human macrophages[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):684.
[3]黄丹丹,鲍朗#,杨晓玲,等.结核分枝杆菌MycP1蛋白的原核表达及其对巨噬细胞的毒性作用*[J].郑州大学学报(医学版),2012,(04):438.
 HUANG Dandan,BAO Lang,YANG Xiaoling,et al.Prokaryotic expression of MycP1 of Mycobacterium tuberculosis and its cytotoxicity to macrophages[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(06):438.
[4]王晓龙),陈新涛),曲戈),等.M1型、M2型巨噬细胞及肿瘤相关巨噬细胞中miR146a表达的差异*[J].郑州大学学报(医学版),2015,(02):225.
 WANG Xiaolong,CHEN Xintao,QU Ge,et al.Expression difference of miRNA146a among M1type,M2type and tumorassociated macrophages[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2015,(06):225.
[5]袁娜娜,卢高峰,唐芙爱#.人结直肠癌组织中肿瘤相关巨噬细胞的检测[J].郑州大学学报(医学版),2013,(01):106.
 YUAN Nana,LU Gaofeng,TANG Fuai.Detection of tumorassociated macrophages in colorectal cancer tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):106.

备注/Memo

备注/Memo:
*国家自然科学基金资助项目30872095,81001239,81001240 #通讯作者,男,1944年11月生,教授,研究方向:分子肿瘤学,Email: wuym@zzu.edu.cn
更新日期/Last Update: 1900-01-01