[1]黄学勇△,许玉玲,李幸乐,等.柯萨奇病毒B5河南分离株全基因组序列测定及分析*[J].郑州大学学报(医学版),2011,(06):868.
 HUANG Xueyong,XU Yuling,LI Xingle,et al.Analysis of genomic characteristics of coxsackie virus B5 strains isolated from Henan province[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):868.
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柯萨奇病毒B5河南分离株全基因组序列测定及分析*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
868
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Analysis of genomic characteristics of coxsackie virus B5 strains isolated from Henan province
作者:
黄学勇许玉玲李幸乐晁灵陈豪敏许汴利
河南省疾病预防控制中心传染病预防控制所 郑州 450016
Author(s):
HUANG XueyongXU Yuling LI XingleCHAO Ling CHEN HaominXU Bianli
Department of Infectious Disease Control and Prevention,Henan Provincial Center for Disease Control and Prevention, Zhengzhou 450016
关键词:
柯萨奇病毒B5全基因组序列基因特征
Keywords:
coxsackie virus B5complete genomegenomic characteristics
分类号:
R373.2+3
摘要:
目的:了解柯萨奇病毒B5(CoxB5)河南分离株全基因特征及与其他肠道病毒基因的关系。方法:采用RTPCR扩增全基因,采用DNAStar中的SeqMan拼接所测序列,BioEdit进行同源性比较,Simplot进行相似度分析。结果:CoxB5/Henan/2010基因组全长7 401 bp。与原型株Faulkner相比,编码区氨基酸同源性为95.6%,核苷酸同源性为79.9%,其中VP4VP2核苷酸差异为17.8%~20.4%,VP1核苷酸差异为19.5%,P2、P3核苷酸差异分别为19.5%、21.7%。不同型别肠道病毒间VP4VP2核苷酸差异为14.4%~34.9%,VP1核苷酸差异为18.3%~42.3%,P2、P3核苷酸差异为17.1%~21.0%、15.5%~22.6%。结论:与原型株相比,CoxB5/Henan/2010株编码区发生沉默突变,其核苷酸变异并不影响氨基酸序列的变化;肠道病毒基因组各分区在进化中不同步。
Abstract:
Aim: To reveal the genomic sequence characteristic of coxsackie virus B5(CoxB5) and its relationship with other enterovirus gene.Methods:The whole genome sequence was sequenced by RTPCR,and BioEdit and Simplot were used to analyze the homology and similarity.Results:The full length of CoxB5/Henan/2010 genome was 7 401 bp.Compared with the prototype strain Faulkner, the entire coding region of amino acid homology was 95.6%,and VP4VP2 nucleotide difference was 17.8%~20.4%, VP1 nucleotide difference was 19.5%,and P2,P3 nucleotide differences were 19.5%,21.7%. VP4VP2 displayed 14.4%~34.9%, VP1 displayed 18.3%~42.3% and P2,P3 displayed 17.1%~21.0%, 15.5%~22.6% in nucleotide difference compared with the other enterovirus, respectively.Conclusion:Compared with the prototype strain, CoxB5/Henan/2010 coding sequence is silent mutation, the nucleotide variation does not affect amino acid sequence changes. The genome evolution is not sync in the district of enterovirus.

参考文献/References:

[1]Oberste MS, Maher K,Pallansch MA. Evidence for frequent recombination within species human enterovirus B based on complete genomic sequences of all thirtyseven serotypes[J].J Virol,2004,78(2):855 [2]Oberste MS,Penaranda S,Pallansch MA. RNA recombination plays a major role in genomic change during circulation of coxsackie B viruses[J].J Virol,2004,78(6):2948 [3]胡永峰,赵丽娜,董杰,等.中国萨科奇病毒B5的全基因组测序及其序列分析[J].病毒学报,2010,26(4):283 [4]陈立,赵月萍,张礼壁,等.引发脑膜脑炎流行的柯萨奇B5病毒的序列分析[J].中国计划免疫,2003,9(2):94 [5]Rotbart HA,Sawyer MH, Fast S,et al. Diagnosis of enteroviral meningitis by using PCR with colorimetric microwell detection assay[J].J Clin Microbiol,1994,32(10):2590 [6]朱谦,许汴利,张锦,等.河南省首起柯萨奇B5病毒性脑炎暴发调查[J].中国公共卫生,2007,23(2):236

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[1]段晶晶),刘国华),黄学勇),等.柯萨奇病毒B5巢式RTPCR检测方法的建立*[J].郑州大学学报(医学版),2013,(03):349.
 DUAN Jingjing),LIU Guohua),HUANG Xueyong),et al.Establishment of nested RTPCR assay for detection of coxsackie virus B5[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2013,(06):349.

备注/Memo

备注/Memo:
*河南省医学科技重大攻关基金资助项目201001015 △男,1971年10月生,博士,副主任医师,研究方向:分子流行病学与传染病预防控制,Email:hxyzzu@163.com
更新日期/Last Update: 1900-01-01