[1]赵小明),薛新宏)#,赵少平).骨肉瘤组织中Ang2、VEGF的表达及MVD测定[J].郑州大学学报(医学版),2011,(06):882.
 ZHAO Xiaoming,XUE Xinhong,ZHAO Shaoping.Detection of expressions of angiopoietin2,VEGF and MVD in osteosarcoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):882.
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骨肉瘤组织中Ang2、VEGF的表达及MVD测定
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
882
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Detection of expressions of angiopoietin2,VEGF and MVD in osteosarcoma tissue
作者:
赵小明1)薛新宏2)#赵少平1)
1)唐山市第二医院骨科 唐山 0630002)河北联合大学附属医院神经内科 唐山 063000
Author(s):
ZHAO Xiaoming1)XUE Xinhong2) ZHAO Shaoping1)
1)Department of Orthopedics,Tangshan Second Hospital, Tangshan 0630002)Department of Neurology, the Affiliated Hospital,Hebei Union University, Tangshan 063000
关键词:
骨肉瘤血管生成素2血管内皮生长因子微血管密度
Keywords:
osteosarcoma angiopoietin2 VEGF microvessel density
分类号:
R738.1
摘要:
目的:检测骨肉瘤组织中血管生成素2(Ang2)与血管内皮生长因子(VEGF)的表达及微血管密度(MVD)。 方法:采用RTPCR技术和Western blot 法检测69例骨肉瘤患者和12例正常对照骨组织中Ang2的表达;采用免疫组化法检测69例骨肉瘤组织中VEGF与CD34的表达,计算MVD;分析Ang2的表达与VEGF、MVD的关系。结果:骨肉瘤组Ang2 mRNA的表达量、Ang2蛋白阳性表达率、VEGF蛋白阳性表达率及MVD均高于对照组(t=8.253,χ2校正=11.960和11.470,P均<0.01)。高VEGF表达组Ang2蛋白与mRNA的表达高于低VEGF表达组(P<0.01);高MVD组Ang2蛋白与mRNA的表达高于低MVD组(P<0.01)。结论:Ang2参与了骨肉瘤的血管生成及调控,在骨肉瘤的发生中起着重要的作用。
Abstract:
Aim: To detect the expressions of angiopoietin2(Ang2),VEGF and microvessel density(MVD) in osteosarcoma(OS) tissue.Methods:RTPCR and Western blot were used to detect the expressions of Ang2 mRNA and protein in bone marrow samples from 69 patients with OS and 12 normal control.The expressions of VEGF and CD34 proteins in OS tissue were detected using immunohistochemistry, and MVD was counted.Results:The mRNA expression level of Ang2,the positive expression rate of Ang2,VEGF,and MVD in OS group were significantly higher than those in control (t=8.253,χ2adj=11.960 and 11.470,P<0.01). The expression of Ang2 was significantly higher in highVEGF expression group than in lowVEGF expression group(P<0.01), and higher in highMVD group than in lowMVD group(P<0.01).Conclusion:Ang2 may be involved in the regulation of angiogenesis in OS,and play an important role in the occurrence of OS.

参考文献/References:

[1]Karamysheva AF. Mechanisms of angiogenesis[J]. Biochemistry (Mosc), 2008,73(7):751 [2]Huang Y, Lin Z, Zhuang J, et al. Prognostic significance of alpha V integrin and VEGF in osteosarcoma after chemotherapy[J]. Onkologie, 2008,31(10):535 [3]Bajpai J, Sharma M, Sreenivas V, et al. VEGF expression as a prognostic marker in osteosarcoma[J]. Pediatr Blood Cancer, 2009,53(6):1035 [4]Quartarone E, Alonci A, Allegra A, et al. Differential levels of soluble angiopoietin2 and Tie2 in patients with haematological malignancies[J]. Eur J Haematol, 2006,77(6):480 [5]Hu B, Cheng SY. Angiopoietin2: development of inhibitors for cancer therapy[J]. Curr Oncol Rep, 2009,11(2):111 [6]Detjen KM, Rieke S, Deters A, et al. Angiopoietin2 promotes disease progression of neuroendocrine tumors[J]. Clin Cancer Res, 2010,16(2):420

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备注/Memo

备注/Memo:
#通讯作者,女,1972年5月生,硕士,副主任医师,研究方向:血栓性疾病的防治,Email: xxhzxm129@sohu.com
更新日期/Last Update: 1900-01-01