[1]罗向阳△,王伟.尿道下裂患儿包皮组织p53和p21waf/cip1 mRNA的表达[J].郑州大学学报(医学版),2011,(06):898.
 LUO Xiangyang,WANG Wei.Expressions of p53 and p21waf/cip1 mRNA in preputial tissue of children with hypospadias[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):898.
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尿道下裂患儿包皮组织p53和p21waf/cip1 mRNA的表达
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
898
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Expressions of p53 and p21waf/cip1 mRNA in preputial tissue of children with hypospadias
作者:
罗向阳王伟
郑州大学第三附属医院泌尿外科 郑州 450052
Author(s):
LUO XiangyangWANG Wei
Department of Urology,the Third Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
p53p21waf/cip1尿道下裂
Keywords:
p53 p21waf/cip1hypospadias
分类号:
R695
摘要:
目的:探讨包皮组织中肿瘤抑制蛋白p53和细胞周期调节基因p21waf/cip1的表达与尿道下裂发病的关系。方法:采用RTPCR技术检测30例尿道下裂患儿和30例对照组患儿包皮组织p53和p21waf/cip1 mRNA表达水平。结果:与对照组比较,尿道下裂组p53 mRNA的表达降低(t=11.431,P=0.010),p21waf/cip1mRNA的表达无变化(t=1.408,P=0.244);不同程度尿道下裂患儿p53和p21waf/cip1的mRNA表达水平差异无统计学意义(F=3.641和3.089,P=0.158和0.187)。结论:p53和p21waf/cip1基因表达的变化可使尿道板和尿道襞上皮细胞过度增殖,尿道板腔化受阻,进而导致尿道下裂的发生。
Abstract:
Aim:To investigate the expressions of p53 and p21waf/cip1 mRNA in preputial tissue of children with hypospadias.Methods:The expressions of p53 and p21waf/cip1 mRNA were measured by semiquantitative reverse transcription polymerase chain reaction (RTPCR) in preputial tissue of children with hypospadias and control children.Results:Compared with the control group, the expression level of p53 mRNA decreased in hypospadias group(t=11.431,P=0.010),and the expression level of p21waf/cip1 mRNA had no change in hypospadias group(t=1.408,P=0.244). The expression levels of p53 and p21waf/cip1 mRNA had no significant difference among the children hypospadias at different lesion degree with (F=3.641 and 3.089,P=0.158 and 0.187).Conclusion:p53 and p21waf/cip1 may be related to the proliferation of prepuce and may contribute to the pathogenesy of hypospadias.

参考文献/References:

[1]陈嘉波,杨体泉.尿道板纵切卷管尿道成形术治疗尿道下裂22例[J].实用儿科临床杂志,2007,22(11):831 [2]Stephens FD,Hutson JM.Differences in embryogenesis of epispadias, exstrophyepispadias complex and hypospadias[J]. J Pediatr Urol,2005,1(4):283 [3]Shetty P,Velusamy T,Bhandary YP,et al.Urokinase expression by tumor suppressor protein p53: a novel role in mRNA turnover[J].Am J Respir Cell Mol Biol,2008,39(3):364 [4]Chen H, Yong W, Hinds TD Jr, et al. Fkbp52 regulates androgen receptor transactivation activity and male urethra morphogenesis[J]. J Biol Chem,2010,285(36):27776 [5]罗向阳,王伟.宫内抗雄性物质暴露对胎鼠及新生大鼠阴茎组织p53和细胞周期调节基因表达的影响[J].实用儿科临床杂志,2011,26(14):1137 [6]Komarova EA,Krivokrysenko V,Wang K,et al.P53 is a suppressor of inflammatory response in mice[J].FASEB J,2005,19(8):1030 [7]Habert R, Muczynski V, Lehraiki A, et al.Adverse effects of endocrine disruptors on the foetal testis development: focus on the phthalates[J].Folia Histochem Cytobiol,2009,47(5):S67

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备注/Memo

备注/Memo:
△男,1968年6月生,副教授,硕士,研究方向:小儿泌尿学畸形,Emali:Luoxy9826@yahoo.com.cn
更新日期/Last Update: 1900-01-01