[1]郭海玲),夏向群),杨海燕)#.机动车尾气有机提取物对HepG2细胞的遗传毒性[J].郑州大学学报(医学版),2011,(06):904.
 GUO Hailing),XIA Xiangqun),YANG Haiyan).Genotoxicity effects of organic extract of vehicle exhaust on HepG2 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):904.
点击复制

机动车尾气有机提取物对HepG2细胞的遗传毒性
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
904
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Genotoxicity effects of organic extract of vehicle exhaust on HepG2 cells in vitro
作者:
郭海玲1)夏向群2)杨海燕3)#
1)北京中医药大学东直门医院护理部 北京100700 2)河南省疾病预防控制中心环境卫生科 郑州 450016 3)郑州大学公共卫生学院流行病学教研室 郑州 450001
Author(s):
GUO Hailing1)XIA Xiangqun2)YANG Haiyan3)
1)Department of Nursing, Dongzhimen Hospital,Beijing University of Traditional Chinese Medicine,Beijing 100700 2)Department of Environment Health,Disease Prevention and Control Center of Henan,Zhengzhou 450016 3)Department of Epidemiology,College of Public Health,Zhengzhou University,Zhengzhou 450001
关键词:
机动车尾气双核细胞微核彗星试验遗传毒性
Keywords:
vehicle exhaustcytokinesisblock micronucleuscomet assay genotoxicity
分类号:
R114
摘要:
目的:探讨机动车尾气有机提取物的体外遗传毒性作用。方法:用双核细胞微核试验和彗星试验评价机动车尾气有机提取物(2.5、5.0和10.0 mg/L)对HepG2细胞的遗传毒性,另设溶剂对照组和阳性对照组,每组6个平行孔。结果:各组双核细胞微核率和Olive尾矩比较,差异有均统计学意义(F=18.608和9.525,P均<0.001)。5.0 和10.0 mg/L有机提取物组双核细胞微核率高于溶剂对照组,3个剂量组Olive尾矩均高于溶剂对照组(P均<0.05)。结论:机动车尾气有机提取物对HepG2细胞具有明显的遗传毒性。
Abstract:
Aim:To explore the genotoxicity of organic extract of vehicle exhaust on HepG2 cells in vitro.Methods:Cytokinesisblock micronucleus assay and comet assay were used to evaluate the genotoxicity of organic extract of vehicle exhaust (2.5,5.0 and 10.0 mg/L) on HepG2 cells.The solvent control group and positive control group were used as control.Results:There were significant differences in frequency of cytokinesisblock micronucleus and Olive tail moment among five groups(F=18.608 and 9.525,P<0.001).A significant increased frequency of cytokinesisblock micronucleus was observed in both 5.0 and 10.0 mg/L dose group compared with the solvent control group(P<0.05).A significant increased Olive tail moment was observed in three dose groups compared with the solvent control group(P<0.05).Conclusion:The organic extract of vehicle exhaust has a distinct genotoxicity on HepG2 cells in vitro.

参考文献/References:

[1]程义斌, 金银龙, 刘迎春. 汽车尾气对人体健康的危害[J].卫生研究, 2003, 32(5): 504 [2]刘文彪, 董胜璋. 机动车尾气慢性毒性效应的研究进展[J].环境与健康杂志, 2002, 19(5): 413 [3]Boettcher M, Grund S, Keiter S, et al. Comparison of in vitro and in situ genotoxicity in the Danube River by means of the comet assay and the micronucleus test[J]. Mutat Res,2010,700(1/2):11 [4]Danielsen PH, Loft S, Kocbach A, et al. Oxidative damage to DNA and repair induced by Norwegian wood smoke particles in human A549 and THP1 cell lines[J].Mutat Res,2009,674(1/2):116 [5]Fenech M. The micronucleus assay determination of chromosomal level DNA damage[J]. Methods Mol Biol,2008,410:185 [6]王亚东,陈小玉,吴逸明, 等. 氯化汞诱导MCF7人乳腺癌细胞增殖的实验研究[J]. 河南医学研究,2005, 14(1):11 [7]曹贤文,杜海荣,张荣,等. 不同预处理消毒饮用水中有机提取物对HepG2细胞的损伤作用[J]. 环境与职业医学,2010(4):198 [8]冷曙光,牛勇,潘祖飞,等. 彗星试验琼脂糖凝胶片保存方法研究[J].卫生研究,2003,32(1):60 [9]朱志良,庄志雄,黄钰,等. 单细胞凝胶电泳图像分析系统的研制及应用[J]. 中华劳动卫生职业病杂志,2001,19(4):298 [10]Fenech M. Cytokinesisblock micronucleus assay evolves into a “cytome” assay of chromosomal instability,mitotic dysfunction and cell death[J]. Mutat Res,2006,600(1/2):58 [11]Zhang Z, Che W, Liang Y, et al. Comparison of cytotoxicity and genotoxicity induced by the extracts of methanol and gasoline engine exhausts[J]. Toxicol In Vitro,2007,21(6):1058 [12]Xu DQ, Zhang WL. Monitoring of pollution of air fine particles (PM2.5) and study on their genetic toxicity[J]. Biomed Environ Sci,2004,17(4):452 [13]Feng S, Kong Z, Wang X, et al. Assessing the genotoxicity of imidacloprid and RH5849 in human peripheral blood lymphocytes in vitro with comet assay and cytogenetic tests[J]. Ecotoxicol Environ Saf,2005,61(2):239 [14]程娟,冷曙光,牛勇,等. 多环芳烃致4种细胞彗星尾矩和双核细胞微核率变化差异的研究[J]. 卫生研究,2008, 37(3):273

备注/Memo

备注/Memo:
#通讯作者,女,1975年6月生,博士,副教授,研究方向:流行病学,Email:yhy@zzu.edu.cn
更新日期/Last Update: 1900-01-01