[1]王存良)△,李远哲).亳菊中木犀草素和木犀草苷含量的反相高效液相色谱法测定[J].郑州大学学报(医学版),2011,(06):912.
 WANG Cunliang,LI Yuanzhe.Determination of luteolin7Oglucoside and luteolin in Chrysanthemum morifolium by RPHPLC[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2011,(06):912.
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亳菊中木犀草素和木犀草苷含量的反相高效液相色谱法测定
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2011年06期
页码:
912
栏目:
论著
出版日期:
2011-11-20

文章信息/Info

Title:
Determination of luteolin7Oglucoside and luteolin in Chrysanthemum morifolium by RPHPLC
作者:
王存良1)李远哲2)
1)郑州大学第一附属医院药剂科 郑州 450052 2)河南工业大学生物制药系 郑州450001
Author(s):
WANG Cunliang1)LI Yuanzhe2)
1)Department of Pharmacy, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Biological Pharmaceutical,Henan University of Technology, Zhengzhou 450001
关键词:
反相高效液相色谱亳菊木犀草素木犀草苷
Keywords:
HPLCChrysanthemum morifoliumluteolinluteolin7Oglucoside
分类号:
R284
摘要:
目的:建立亳菊中木犀草素和木犀草苷含量的反相高效液相色谱测定方法。方法:采用反相高效液相色谱法,色谱柱为Scienhome C18 (4.6 mm×200 mm,5 μm),检测波长350 nm,柱温30 ℃,流速1 mL/min。结果:木犀草苷含量在0.004 8~0.038 4 μg内线性良好,Y=10 000 000X-6 650,r=0.999 9,回收率为104.9%,RSD为1.64%;木犀草素含量在0.032~0.256 μg内线性良好,Y=8 000 000X-20 581,r=0.999 9,回收率为101.7%,RSD为1.68%。结论:该测定方法准确可靠,重现性好,对亳菊的质量控制有重要意义。
Abstract:
Aim:To develop a RPHPLC method for the determination of luteolin7Oglucoside and luteolin in Chrysanthemum morifolium.Methods:RPHPLC was carried out on a Purospher star Scienhome C18 (4.6 mm×200 mm,5 μm) at 30 ℃, at a flow rate of 1 mL/min.The detection wavelength was 350 nm.Results:The linearity of luteolin7Oglucoside was found in the range of 0.004 8~0.038 4 μg,Y=10 000 000X-6 650,r=0.999 9, the average recovery was 104.9%,and RSD was 1.64%.The linearity of luteolin was found in the range of 0.032~0.256 μg,Y=8 000 000X-20 581,r=0.999 9, the average recovery was 101.7%,and RSD was 1.68%.Conclusion:This method is reliable,accurate and suitable for the determination of luteolin7Oglucoside and luteolin in Chrysanthemum morifolium, and plays an important role in the quality control of Chrysanthemum morifolium.

参考文献/References:

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备注/Memo

备注/Memo:
△男,1973年2月生,主管药师,研究方向:药理学,Email:wangcunliang730217@163.com
更新日期/Last Update: 1900-01-01