[1]翟文杰,翟明霞,吕虹,等.结核分枝杆菌CFP21抗原的HLAA*0201/*03限制性细胞毒T淋巴细胞表位预测*[J].郑州大学学报(医学版),2012,(01):10.
 ZHAI Wenjie,ZHAI Mingxia,LV Hong,et al.Prediction of HLAA*0201/*03restricted cytotoxic T lymphocyte epitopes in Mycobacterium tuberculosis CFP21 antigen[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):10.
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结核分枝杆菌CFP21抗原的HLAA*0201/*03限制性细胞毒T淋巴细胞表位预测*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
10
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Prediction of HLAA*0201/*03restricted cytotoxic T lymphocyte epitopes in Mycobacterium tuberculosis CFP21 antigen
作者:
翟文杰翟明霞吕虹祁元明高艳锋#
郑州大学生物工程系 郑州 450001
Author(s):
ZHAI Wenjie ZHAI Mingxia LV Hong QI Yuanming GAO Yanfeng
Department of Bioengineering,Zhengzhou University,Zhengzhou 450001
关键词:
结核分枝杆菌CFP21表位
Keywords:
Mycobacterium tuberculosisCFP21epitope
分类号:
R52
摘要:
目的:探寻结核分枝杆菌CFP21抗原的同时针对HLAA*0201/*03型别的广谱细胞毒T淋巴细胞(CTL)表位。方法:对结核分枝杆菌CFP21抗原的HLAA*0201候选肽,利用在线数据库预测HLAA*03限制性的天然表位肽,通过氨基酸置换适当修饰获得对应的改造肽。通过T2A3细胞结合力实验筛选候选肽,用于细胞毒活性实验。结果:在线数据库预测共获得4条母体肽和3条改造肽。结合力实验显示,改造肽p1341Y2L、p1341Y2L9L和母体肽p134与HLAA*03分子均具有较高的结合力。改造肽p1341Y2L9L和母体肽p134均能诱导CTL反应,但p134诱导出的CTL对靶细胞具有更强的杀伤效应。结论:p134(AVADHVAAV)是同时针对HLAA*0201/*03型别的广谱CTL表位。
Abstract:
Aim:To explore the HLAA*0201/*03 cytotoxic T lymphocyte(CTL) epitopes from Mycobacterium tuberculosis CFP21.Methods:The online database was applied to predict the HLAA*03 restricted natural epitope peptide based on the HLAA*0201 candidate peptides of CFP21 antigen,and modified epitopes were obtained by amino acid replacement. T2A3 cell line was used to determine the binding affinity of the candidate peptides,and the candidate peptides were selected for subsequent cytotoxicity assay.Results:Four native epitopes and 3 modified epitopes were obtained.The binding affinity of the modified epitope p1341Y2L, p1341Y2L9L and the native p134 towards HLAA*03 molecule was relatively high.Cytotoxicity assay showed that both the modified epitope p1341Y2L9L and the native peptide p134 could induce potent CTL response,and p134induced CTLs could potently lyse the peptideloaded target cells.Conclusion:p134(AVADHVAAV) could be a broad spectrum CTL epitope towards HLAA*0201/*03.

参考文献/References:

[1]Rajasekaran S,Chandrasekar C,Mahilmaran A,et al.HIV coinfection among multidrug resistant and extensively drug resistant tuberculosis patients—a trend[J].J Indian Med Assoc,2009,107(5):281 [2]Roy E,Lowrie DB,Jolles SR.Current strategies in TB immunotherapy[J].Curr Mol Med, 2007,7(4):373 [3]Grover A, Ahmed MF, Verma I, et al. Expression and purification of the Mycobacterium tuberculosis complexrestricted antigen CFP21 to study its immunoprophylactic potential in mouse model[J]. Protein Expr Purif, 2006,48(2):274 [4]吕虹,高艳锋,祁元明. 结核杆菌抗原CFP21/MTB12/Rv3881c的HLA限制性细胞毒T淋巴细胞表位预测[J].郑州大学学报:医学版, 2010,45(1):78 [5]Lv H, Gao Y, Wu Y, et al. Identification of a novel cytotoxic T lymphocyte epitope from CFP21, a secreted protein of Mycobacterium tuberculosis[J]. Immunol Lett, 2010,133(2):94 [6]Wang B, Yao K, Liu G, et al. Computational prediction and identification of EpsteinBarr virus latent membrane protein 2A antigenspecific CD8+Tcell epitopes[J]. Cell Mol Immunol, 2009,6(2):97 [7]Lazoura E, Apostolopoulos V. Rational Peptidebased vaccine design for cancer immunotherapeutic applications[J]. Curr Med Chem,2005,12(6):629 [8]Li F,Yang D,Wang Y,et al.Identification and modification of an HLAA*0201restricted cytotoxic T lymphocyte epitope from Ran antigen[J].Cancer Immunol Immunother, 2009,58(12):2039 [9]Ding FX,Wang F,Lu YM,et al.Multiepitope peptideloaded viruslike particles as a vaccine against hepatitis B virusrelated hepatocellular carcinoma[J].Hepatology, 2009,49(5):1492 [10]Aoshi T, Nagata T, Suzuki M, et al. Identification of an HLAA*0201restricted Tcell epitope on the MPT51 proteina major secreted protein derived from Mycobacterium tuberculosis, by MPT51 overlapping peptide screening[J]. Infect Immun, 2008,76(4):1565 [11]Hovav AH,Fishman Y,Bercovier H.Gamma interferon and monophosphory lipid Atrehalose dicorynomycolate are efficient adjuvants for Mycobacterium tuberculosis multivalent acellular vaccine[J].Infect Immun, 2005,73(1):250 [12]Sablel SB,Kalra M,Verma I,et al.Tuberculosis subunit vaccine design:the conflict of antigenicity and immunogenicity[J].Clin Immunol, 2007,122(3):239 [13]陈燕,娄加陶,吴传勇,等.KLIANNTRV是结核抗原Ag85A的HLAA*0201限制性CTL 表位[J].现代检验医学杂志,2007,22(5):1 [14]吴传勇,娄加陶,蒋廷旺,等.结核杆菌抗原Ag85C的HLAA*0201限制性CD8+ CTL表位的预测及鉴定[J].第二军医大学学报, 2007,28(4):349 [15]Frieder M,Lewinsohn DM.Tcell epitope mapping in Mycobacterium tuberculosis using pepmixes created by microscale SPOTsynthesis[J].Methods Mol Biol, 2009,524:369

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30872381;30901362 doi:10.3969/j.issn.16716825.2012.01.004 #通讯作者,男,1980年10月生,博士,副教授,研究方向:抗原肽和多肽疫苗,Email:gaoyf@zzu.edu.cn
更新日期/Last Update: 1900-01-01