[1]张楠楠),潘卫东),郑国庭),等.甘蓝型油菜二酰甘油酰基转移酶基因微藻真核表达载体的构建*[J].郑州大学学报(医学版),2012,(01):14.
 ZHANG Nannan,PAN Weidong,ZHENG Guoting,et al.Construction of eukaryotic expression vector for microalga using cloned DGAT genes from Brassica napus[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):14.
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甘蓝型油菜二酰甘油酰基转移酶基因微藻真核表达载体的构建*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
14
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Construction of eukaryotic expression vector for microalga using cloned DGAT genes from Brassica napus
作者:
张楠楠1)潘卫东2)郑国庭3)李靓1)崔玉琳4)秦松5)薛乐勋1)#
1)郑州大学生物工程系细胞生物学研究室 郑州 450001 2)郑州大学基础医学院微生物学与免疫学教研室 郑州 450001 3)宁波大学海洋生物技术重点实验室 宁波 315211 4)中国科学院海洋研究所 青岛 266071 5)中国科学院烟台海岸带研究所 烟台 264003
Author(s):
ZHANG Nannan1)PAN Weidong2)ZHENG Guoting3) LI Liang1) CUI Yulin4) QIN Song5) XUE Lexun1)
1)Laboratory for Cell Biology,Department of Bioengineering, Zhengzhou University, Zhengzhou 450001 2)Department of Microbiology and Immunology,College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450001 3)Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211 4)Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071 5)Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003
关键词:
甘蓝型油菜二酰甘油酰基转移酶微藻真核表达载体
Keywords:
Brassica napusdiacylglycerol acyltransferasemicroalgaeukaryotic expression vector
分类号:
Q782
摘要:
目的:构建二酰甘油酰基转移酶(DGAT)的微藻真核表达载体。方法:采用RTPCR从甘蓝型油菜中扩增得到DGAT1和DGAT2 2个基因cDNA编码区序列,分别连接到载体pMD18T Simple上,经测序及与已知的DGAT1和DGAT2序列比对,将鉴定正确的基因用于微藻真核表达载体pSV40DGAT1/CaMVBar和pSV40DGAT2/CaMVBar的构建。结果:克隆到的甘蓝型油菜DGAT1和DGAT2 2个基因长度分别为1 512和1 026 bp,同源性分别为99%和98%,构建了微藻真核表达载体pSV40DGAT1/CaMVBar和pSV40DGAT2/CaMVBar。结论:2个微藻真核表达载体构建成功。
Abstract:
Aim:To construct the diacylglycerol acyltransferase(DGAT) eukaryotic expression vector for microalga.Methods:DGAT1 and DGAT2 were obtained from Brassica napus through RTPCR and then cloned into vector pMD18T Simple. The cDNA fragments indentified correctly by sequencing were subcloned into an eukaryotic expression vector to construct pSV40DGAT1/CaMVBar and pSV40DGAT2/CaMVBar.Results:The cloned cDNA sequences were 1 512 and 1 026 bp with hemology of 99% and 98%,respectively, and were utilized for constructing eukaryotic expression vectors pSV40DGAT1/CaMVBar and pSV40DGAT2/CaMVBar.Conclusion:The two eukaryotic expression vectors for microalga have been successfully constructed.

参考文献/References:

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备注/Memo

备注/Memo:
*国家自然科学基金资助项目30700014;科技部国际科技合作基金资助项目2007DFA01240 doi:10.3969/j.issn.16716825.2012.01.005 #通讯作者,男,1944年2月生,教授,博士研究生导师,研究方向:肿瘤标志物与基因工程,Email:xuelx@zzu.edu.cn
更新日期/Last Update: 1900-01-01