[1]王璐,朱莹,袁耀东,等.皮肤鳞状细胞癌组织中埃兹蛋白和水通道蛋白3的表达*[J].郑州大学学报(医学版),2012,(01):23.
 WANG Lu,ZHU Ying,YUAN Yaodong,et al.Expressions of Ezrin and AQP3 in skin squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):23.
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皮肤鳞状细胞癌组织中埃兹蛋白和水通道蛋白3的表达*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
23
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Expressions of Ezrin and AQP3 in skin squamous cell carcinoma tissue
作者:
王璐朱莹袁耀东刘林嶓#
郑州大学第一附属医院整形外科 郑州 450052
Author(s):
WANG LuZHU YingYUAN YaodongLIU Linbo
Department of Plastic Surgery, the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052
关键词:
埃兹蛋白水通道蛋白3鳞状细胞癌皮肤肿瘤
Keywords:
Ezrinaquaporins3squamous cell carcinomaskin neoplasm
分类号:
R739.5
摘要:
目的:探讨埃兹蛋白(Ezrin)和水通道蛋白3(AQP3)在皮肤鳞状细胞癌(SCC)组织中的表达及临床意义。方法:应用免疫组化染色SP法检测65例皮肤SCC(患者中男36例,女29例;高分化24例,中分化23例,低分化18例;有颈淋巴结转移9例;均无合并症且未经治疗)和20例正常皮肤组织中Ezrin和AQP3的表达情况。结果:SCC组织中Ezrin、AQP3阳性表达率分别为81.5%(53/65)和86.2%(56/65),2者在正常皮肤组织中的阳性表达率均为5.0%(1/20),SCC组织中Ezrin和AQP3阳性表达率高于正常皮肤组织(χ2分别为38.669,45.598,P均<0.001)。Ezrin的表达与SCC的分化程度有关,分化程度越低阳性表达率越高(P=0.024);且与淋巴结转移无关(χ2校正=0.022,P=0.881)。AQP3蛋白的表达与SCC的分化程度有关,随SCC分化程度的增高而升高(P=0.012);与淋巴结转移无关(χ2校正<0.001,P>0.999)。SCC组织中Ezrin和AQP3的表达呈正关联(rP=0.446,P<0.001)。结论:Ezrin和AQP3的高表达可能参与了皮肤SCC的发生、发展和转移,并与其恶性程度相关。
Abstract:
Aim: To investigate the expressions and the clinical significance of Ezrin and aquaporins3(AQP3) proteins in skin squamous cell carcinoma(SCC) tissue.Methods:Ezrin and AQP3 proteins were detected in 65 cases of skin SCC(36 males,29 females;24 cases of well differentiated,23 cases of moderately differentiated and 18 cases of poorly differentiated;9 cases with lymph node metastasis;all without complications and treatment) and 20 normal skin specimens by SP immunohistochemical technique.Results:The positive expression rates of Ezrin and AQP3 in SCC were 81.5%(53/65) and 86.2%(56/65),both of them in normal skin tissue was 5.0%.The positive expression rates of Ezrin and AQP3 in SCC were higher than those in normal skin tissue (χ2=38.669,45.598,P<0.001).In SCC,the expression of Ezrin increased from highly differentiated to poorly differentiated tissue (P=0.024),and was not related to lymph node metastasis(χ2adj=0.022,P=0.881).The positive expression rate of AQP3 decreased from highly differentiated to poorly differentiated tissue (P=0.012),and was not related to lymph node metastasis(χ2adj<0.001,P>0.999). The expression of Ezrin was positively associated with that of AQP3 in SCC tissue(rP=0.446,P<0.001).Conclusion:The high expressions of Ezrin and AQP3 proteins may contribute to the pathogenesis and progression and metastasis,and malignant degree of skin SCC.

参考文献/References:

[1]Christenson LJ,Borrowman,Vachon CM,et al. Incidence of baseal cell and squamous cell carcinoma in a population younger than 40 years[J]. JAMA,2005,294(6):681 [2]Valdman A,Fang X,Pang ST,et al.Ezrin expression in prostate cancer and benign prostate tissue[J]. Eur Urol,2005,48(5):852 [3]Takata K,Matsuzaki T,Tajika Y.Aquaporins: water channel proteins of the cell membrane[J]. Prog Histochem Cytochem,2004,39(1):1 [4]King LS,Yasui M,Agre P.Aquaporins in health and disease[J].Mol Med Today,2000,6(2):60 [5]李留霞,赵艳萍,文建国,等.卵巢上皮性癌组织中水通道蛋白3的表达[J].郑州大学学报:医学版,2008,43(2):329 [6]李真珍,文建国,张红,等.轻度肾积水儿童肾脏水通道蛋白2、3和4的表达[J].实用儿科临床杂志,2007,22(22):1751 [7]Moilanen J,Lassus H,Leminen A,et al.Ezrin immunoreactivity in relation to survival in serous ovarian carcinoma patients[J]. Gynecol Oncol,2003,90(2):273 [8]Pujuguet P,Del Maestro L,Gautreau A,et al.Ezrin regulates Ecadherindependent adherens junction assembly through Rac1 activation[J]. Mol Biol Cell,2003,14(5):2 181 [9]Khanna C,Wan X,Bose S,et al.The membrane cytoskeleton linker ezrin is necessary for osteosarcoma metastasis[J]. Nat Med,2004,10(2):182 [10]Luciani F,Matarrese P,Giammarioli AM,et al. CD95/phosphorylated ezrin association underlies HIV1 GP120/IL2induced susceptibility to CD95 (APO1/Fas)mediated apoptosis of human resting CD4(+) T lymphocytes[J].Cell Death Differ,2004,11(5):574 [11]Morishita Y,Sakube Y,Sasaki S,et al. Molecular mechanisms and drug development in aquaporin water channel diseases: aquaporin superfamily(superaquaporins): expansion of aquaporins restricted to multicellular organisms[J].Pharmacol Sci,2004,96(3):276 [12]Sougrat R,Morand M,Gondran C,et al.Functional expression of AQP3 in human skin epidermis and reconstrueted epidermis[J].J Invest Dermatol,2002,118(4):678 [13]HaraChikuma M,Verkman AS.Prevention of skin tumorigenesis and impairment of epidermal cell proliferation by targeted aquaporin3 disruption[J]. Mol Cell biol,2008,28(1):326 [14]Xiang Y,Ma B,Li T,et al.Acetazolamide suppresses tumor metastasis and related protein expression in mice bearing lewis lung carcinoma[J].Acta Pharmacol Sin,2002,23(8):745

备注/Memo

备注/Memo:
*河南省医学科技攻关基金资助项目200703028 doi:10.3969/j.issn.16716825.2012.01.008 #通讯作者,男,1960年3月生,教授,研究方向:体表肿瘤的防治,Email:liulinbo@zzu.edu.cn
更新日期/Last Update: 1900-01-01