[1]赵焕焕),贾陆),裴迎新)#.黄秋葵粗多糖体外抗氧化活性测定[J].郑州大学学报(医学版),2012,(01):40.
 ZHAO Huanhuan,JIA Lu,PEI Yingxin.Determination of antioxidant activity of okra raw polysaccharides in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):40.
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黄秋葵粗多糖体外抗氧化活性测定
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
40
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Determination of antioxidant activity of okra raw polysaccharides in vitro
作者:
赵焕焕1)贾陆2)裴迎新1)#
1)郑州大学公共卫生学院营养与食品卫生学教研室 郑州 450001 2)郑州大学药学院生药学教研室 郑州 450001
Author(s):
ZHAO Huanhuan1)JIA Lu2)PEI Yingxin1)
1)Department of Nutrition and Food Hygiene,College of Public Health, Zhengzhou University, Zhengzhou 450001 2)Department of Natural Products Chemistry,College of Pharmacy,Zhengzhou University, Zhengzhou 450001
关键词:
黄秋葵多糖抗氧化活性
Keywords:
okrapolysaccharideantioxidant activity
分类号:
TQ461
摘要:
目的:测定黄秋葵粗多糖(RPS)的体外抗氧化活性。方法:采用水提醇沉淀法提取黄秋葵RPS,并用蒽酮硫酸法测定RPS的含量,应用1,1二苯基苦味酰基苯肼(DPPH)氧化法检测0.086、0.171、0.342、0.684、1.367和2.734 g/L RPS对 DPPH自由基(DPPH•)的清除率,水杨酸法检测0.157、0.313、0.625、1.250、2.500和5.000 g/L RPS对羟基自由基(•OH)的清除率,邻苯三酚自氧化法检测0.086、0.171、0.342、0.684、1.367和2.734 g/L RPS对超氧阴离子自由基(O2÷)的清除率,以维生素C作阳性对照。结果:黄秋葵中RPS含量为(11.23±0.04)%。RPS对DPDH•、•OH和O2÷的体外清除率呈现剂量依赖性。2.734 g/L RPS对DPPH•和O2÷的清除率分别达(44.48±0.64)%及(35.67±0.21)%,5.000 g/L RPS对•OH的清除率达(68.63±0.07)%。其清除能力较维生素C稍弱。结论:黄秋葵RPS具有较明显的体外抗氧化能力。
Abstract:
Aim:To study the antioxidant activity of okra raw polysaccharides(RPS) in vitro.Methods:RPS was extracted from fresh okra by hot water, then precipitated by alcohol, and its total sugar content was determined by the anthronesulfuric acid method. 1,1dipheny2picrylhydrazyl (DPPH) oxidation method was adopted for measuring the DPPH free radicals (DPPH•) clearance of 0.086,0.171,0.342,0.684,1.367,2.734 g/L RPS, salicylic acid method was adopted for measuring hydroxyl radicals (•OH) clearance of 0.157,0.313,0.625,1.250,2.500,5.000 g/L RPS, pyrogallol autoxidation method was adopted for determining superoxide anion free radicals (O2÷) clearance of 0.086,0.171,0.342,0.684,1.367,2.734 g/L RPS.Vitamin C was selected as a positive control.Results:The content of RPS was (11.23±0.04)%.The scavenging effects of RPS had certain dosagedependent trend and was lower than that of Vitamin C.When the concentration of RPS was 2.734 g/L,the clearance rates of DPPH•and O2÷ were (44.48±0.64)% and(35.67±0.21)%,respectively. The clearance rate of •OH was (68.63±0.07)% when the concentration was 5.000 g/L.Conclusion:Okra RPS exhibit obvious antioxidant activity in vitro.

参考文献/References:

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备注/Memo

备注/Memo:
doi:10.3969/j.issn.16716825.2012.01.013 #通讯作者,女,1955年11月生,博士,教授,研究方向:营养与疾病,Email:pyx@zzu.edu.cn
更新日期/Last Update: 1900-01-01