[1]李江),万鼎铭)#.急性白血病患者骨髓单个核细胞中BAG1及Bcl2蛋白的表达[J].郑州大学学报(医学版),2012,(01):57.
 LI Jiang,WAN Dingming.Expressions of BAG1 and Bcl2 proteins in bone marrow mononuclear cells from patients with acute leukemia[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):57.
点击复制

急性白血病患者骨髓单个核细胞中BAG1及Bcl2蛋白的表达
分享到:

《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
57
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Expressions of BAG1 and Bcl2 proteins in bone marrow mononuclear cells from patients with acute leukemia
作者:
李江12)万鼎铭1)#
1)郑州大学第一附属医院血液科 郑州 450052 2)河南职工医学院内科教研室 郑州 451191
Author(s):
LI Jiang12)WAN Dingming1)
1)Department of Hematology,the First Affiliated Hospital,Zhengzhou University, Zhengzhou 450052 2)Department of Internal Medicine,Henan Medical College of Staff and Workers,Zhengzhou 451191
关键词:
BAG1Bcl2急性白血病
Keywords:
Bcl2 associated athanogeneBcl2acute leukemia
分类号:
R733.71
摘要:
目的:探讨急性白血病(AL)患者骨髓单个核细胞中BAG1和Bcl2蛋白的表达及意义。方法:用免疫细胞化学SP染色法分别检测初治AL患者(112例)、急性白血病完全缓解 (ALCR)患者(57例)、急性白血病难治复发(RRAL)患者(29例)及正常对照者(20例)的骨髓单个核细胞中BAG1及Bcl2蛋白的表达。结果:4组间BAG1及Bcl2蛋白阳性表达率差异有统计学意义(χ2=61.795、37.866,P<0.001),初治AL组和RRAL组中2者的阳性表达率均高于ALCR组和正常对照组(P<0.001)。初治AL患者骨髓单个核细胞中BAG1蛋白阳性表达率与白细胞计数及髓外浸润有关(χ2=16.336、19.289,P<0.001),且BAG1与Bcl2的表达呈正关联(rP=0.587,P<0.001)。结论:BAG1可能协同Bcl2参与了急性白血病的发生发展。
Abstract:
Aim:To explore the BAG1 and Bcl2 expressions in bone marrow mononuclear cells from patients with acute leukemia(AL).Methods:The expressions of BAG1 and Bcl2 in bone marrow mononuclear cells were detected by SP immunocytochemical staining assay in untreated AL group (112 cases),AL complete remission(ALCR) group(57 cases), relapsing and refractory AL(RRAL) group (29 cases), and normal control group(20 cases).Results:There were significant differences in the positive expression rates of BAG1 and Bcl2 proteins among the 4 groups (χ2=61.795,37.866,P<0.001),and the positive rates of the two proteins in untreated AL group and RRAL group were significantly higher than those in ALCR group and normal control group (P<0.001).In untreated group,there was a marked relation between the expression of BAG1 and the number of WBC in peripheral blood or leukemic infiltration(χ2=16.336,19.289,P<0.001),and BAG1 positive rate was associated with Bcl2(rP=0.587,P<0.001).Conclusion:BAG1 combined with Bcl2 may play an important role in the pathogenesis and progression of AL.

参考文献/References:

[1]Townsend PA, Cutress RI, Sharp A, et al. BAG1:a multifunctional regulator of cell growth and survival[J].Biochim Biophys Acta,2003,1603(2):83 [2]Tang SC,Beck J,Murphy S,et al.BAG1 expression correlates with Bcl2,p53,differentiation,estrogen and progesterone receptors in invasive breast carcinoma[J].Breast Cancer Res Treat,2004,84(3):203 [3]Krajewska M,Turner BC,Shabaik A,et al.Expression of BAG1 protein correlates with aggressive behavior of prostate cancers[J].Prostate,2006,66(8):801 [4]Barnes JD,Arhel NJ,Lee SS,et al.Nuclear BAG1 expression inhibits apoptosis in colorectal adenomaderived epithelial cells[J].Apoptosis,2005,10(2):301 [5]邱根全,赵云,刘日失,等.白术对小鼠移植性肉瘤S180的抑瘤作用及对Bcl2基因表达的影响[J].西安交通大学学报:医学版,2009,30(6):759 [6]李洪梅,杨华,周小鸽,等.年轻人霍奇金淋巴瘤亚型特点及肿瘤细胞EB病毒和Bcl2蛋白的表达及意义[J].吉林大学学报:医学版,2011,37(6):1111 [7]唐成和,李树军, 李明超,等.血管内皮生长因子与Bcl2基因在急性淋巴细胞白血病的表达及意义[J].实用儿科临床杂志,2007,22(3):183 [8]林敏,谢海龙,苏琦,等.二烯丙基二硫作用于HL60细胞后凋亡相关基因的差异表达[J].癌症,2007,26(4):351 [9]Xiong J,Chen J,Chernenko G,et al.Antisense BAG1 sensitizes HeLa cells to apoptosis by multiple pathways[J].Biochen Biophys Res Commun,2003,312(3):585 [10]Antoku K, Maser RS, Scully WJ Jr, et al. Isolation of Bcl2 binding proteins that exhibit homology with BAG1 and suppressor of death domains protein[J].Biochem Biophys Res Commun,2001,286(5):1003 [11]Tothova E,Stecova N,Kafkova A,et al.Relation between Bcl2 protein expression and results of therapy in patients with acute myeloblastic leukemia[J].Vnitr Lek,2004,50(2):139 [12]Hartung L,Bahler DW. Flow cytometric analysis of Bcl2 can distinguish small numbers of acute lymphoblastic leukemia cells from Bcell precursors[J].Br J Haematol,2004,127(1):50 [13]Daneshbod Y,Amirqhofran Z,Tabei SZ. Bcl2 expression in acute myelogenous leukemia: the relation to myeloid antigen expression and response to therapy in Iranian patients[J].Neoplasma,2005,52(2):109

相似文献/References:

[1]魏攀,李杰,王莉莉,等.转染杜氏盐藻1433基因对食管鳞癌EC9706细胞Bcl2和Caspase9蛋白表达的影响*[J].郑州大学学报(医学版),2010,(01):4.
 WEI Pan,LI Jie,WANG Lili,et al.Expression of Bcl2 and Caspase9 proteins of EC9706 cell transfected 1433 gene of Dunaliella salina[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):4.
[2]庞霞,郑湘予,李晟磊,等.食管鳞癌组织中半乳糖凝集素3和Bcl2蛋白的表达*[J].郑州大学学报(医学版),2010,(01):8.
 PANG Xia,ZHENG Xiangyu,LI Shenglei,et al.Expression of Galectin3 and Bcl2 protein in esophageal squamous cell carcinoma tissue[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):8.
[3]马俊芬,李沛,黄幼田,等.曲古菌素A对食管癌细胞系EC1细胞凋亡的影响*[J].郑州大学学报(医学版),2010,(02):180.
 MA Junfen,LI Pei,HUANG Youtian,et al.Effects of trichostatin A on apoptosis in esophageal carcinoma cell line EC1 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):180.
[4]夏宗江,赵松,崔广晖#.非小细胞肺癌组织中Survivin、Bcl2及PCNA蛋白的表达[J].郑州大学学报(医学版),2010,(02):322.
[5]常方方,高明,周云#.奥沙利铂联合顺铂对体外培养BGC823细胞增殖及Bcl2、Bax、Caspase3蛋白表达的影响[J].郑州大学学报(医学版),2009,(02):359.
 CHANG Fangfang,GAO Ming,ZHOU Yun.Effects of OXA and DDP on cell proliferation and Bcl2,Bax,Caspase3 expression of BGC823 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):359.
[6]吴大鹏)△,姜睿斌),赵德强),等.β榄香烯乳对体外培养Tca8113细胞放射增敏、细胞周期、凋亡及Bcl2、Bax蛋白表达的影响[J].郑州大学学报(医学版),2009,(02):414.
 WU Dapeng),JIANG Ruibin),ZHAO Deqiang),et al.Effects of βelemene on radiosensitivity, cell cycle, apoptosis, Bcl2 and Bax expression of Tca8113 cells in vitro[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):414.
[7]陈吉相△,赵婷,黄丹霞,等.凝血酶预处理对大鼠神经细胞凋亡的影响[J].郑州大学学报(医学版),2009,(04):781.
 CHEN Jixiang,ZHAO Ting,HUANG Danxia,et al.Effect of thrombin preconditioning of neurocytes apoptosis of rats[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):781.
[8]田卫红),田芳),许培荣),等.HeLa细胞系中核转录因子κB与Bcl2的表达*[J].郑州大学学报(医学版),2009,(01):74.
 TIAN Weihong),TIAN Fang),XU Peirong),et al.Correlation between nuclear factorkappa B and Bcl2 in cervical cancer cell cervical carcinoma[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):74.
[9]臧卫周△,杨红旗,徐军,等.姜黄素对缺氧复氧损伤PC12细胞的影响[J].郑州大学学报(医学版),2009,(03):594.
 ZANG Weizhou,YANG Hongqi,XU Jun,et al.Effects of curcumin on PC12 cells impaired by mimic ischemia and reperfusion[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2009,(01):594.
[10]韩萍)△,赵妍娟),裴兰英),等.顺铂联合葛根素纯品和葛根粗提物对H446细胞增殖及Bax、Bcl2蛋白表达的影响[J].郑州大学学报(医学版),2010,(03):477.
 HAN Ping),ZHAO Yanjuan),PEI Lanying),et al.Effects of cisplatin combined with pure puerarin or pueraria crude extract on proliferation,expressions of Bax and Bcl2 of H446 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2010,(01):477.

备注/Memo

备注/Memo:
doi:10.3969/j.issn.16716825.2012.01.018 #通讯作者,男,1963年5月生,博士,主任医师,研究方向:造血系统疾病的基础与临床,Email:wwddmm@vip.sina.com
更新日期/Last Update: 1900-01-01