[1]王小稳△.淫羊藿总黄酮对病毒性心肌炎小鼠血清免疫调节因子水平的影响[J].郑州大学学报(医学版),2012,(01):60.
 WANG Xiaowen.Influence of total flavones of epimedium on serum levels of cytokines of viral myocarditis mouse[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):60.
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淫羊藿总黄酮对病毒性心肌炎小鼠血清免疫调节因子水平的影响
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
60
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Influence of total flavones of epimedium on serum levels of cytokines of viral myocarditis mouse
作者:
王小稳
郑州市儿童医院肾病风湿科 郑州 450053
Author(s):
WANG Xiaowen
Department of Nephropathy Rheumatology,Children's Hospital of Zhengzhou City,Zhengzhou 450053
关键词:
淫羊藿总黄酮病毒性心肌炎IFNγ小鼠白细胞介素
Keywords:
total flavones of epimediumviral myocarditisIFNγmouseinterleukin
分类号:
R542.2
摘要:
目的:观察淫羊藿总黄酮(TFE)对病毒性心肌炎小鼠免疫功能的影响。方法:雄性BALB/C小鼠32只,随机分为正常组、模型组、TFE高剂量组和TFE低剂量组,每组8只。模型组和TFE高、低剂量组采用腹腔接种柯萨奇病毒B3的方法建立病毒性心肌炎模型。末次接种1 h后,正常组及模型组小鼠灌胃羧甲基纤维素钠溶液,TFE高、低剂量组分别灌胃200、100 mg/kg TFE。连续灌胃14 d后,测定心肌肥厚指数,HE染色法观察心肌病理变化,比色法测定血清LDH、CK水平,ELISA法检测血清IFNγ、IL2、IL4、IL10水平。结果:4组小鼠心肌肥厚指数,血清LDH、CK、IFNγ、IL2、IL4及IL10水平差异均有统计学意义(F=42.895,105.240,96.955,15.679,12.661,25.453,13.925,P<0.001)。与正常组比较,模型组小鼠心肌发生明显病理改变,心肌肥厚指数和血清LDH、CK、IFNγ、IL2水平上升,而IL4、IL10水平下降(P<0.05)。与模型组比较,2个TFE组心肌病理变化减轻,心肌肥厚指数和血清LDH、CK、IFNγ、IL2水平降低,IL4、IL10水平升高(P<0.05)。高剂量组效果较明显。结论:TFE可通过免疫调节保护病毒性心肌炎小鼠心脏。
Abstract:
Aim:To observe the influence of total flavones of epimedium(TFE) on immunological regulation of viral myocarditis mice.Methods:A total of 32 male BALB/C mice were randomly divided into normal, model, TFE highdose and TFE lowdose groups,and eight in each group. The viral myocarditis model was established with intraperitoneal inoculation of coxsackie virus B3 in model, TFE highdose and TFE lowdose groups.1 h after the last inoculation,carboxymethlylcellulose sodium solution was irrigated to the mice in model and normal groups.200 and 100 mg/kg TFE was irrigated to the mice in TFE high and lowdose groups for 14 d.Myocardial hypertrophy index was detected,pathologic changes of myocardium were observed with HE staining,serum LDH and CK levels were assayed with colorimetric method,and serum IFNγ,IL2, IL4, IL10 levels were determined by ELISA method.Results:There were significant differences in these indices mentioned above among the four groups(F=42.895,105.240,96.955,15.679,12.661,25.453,13.925,P<0.05).Compared with those of the normal group, the pathologic changes of myocardium happened, myocardial hypertrophy index, serum LDH, CK, IFNγ, IL2 levels obviously rised, while serum IL4, IL10 levels significantly decreased in the model group(P<0.05).Compared with those of the model group,the pathologic changes of myocardium improved, the myocardial hypertrophy index, serum LDH, CK, IFNγ,IL2 levels decreased, and serum IL4, IL10 levels increased in the two TFE groups(P<0.05). The effectiveness of TFE highdose group was more significant.Conclusion: TFE could protect the hearts of viral myocarditis mice through immunological regulation.

参考文献/References:

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备注/Memo

备注/Memo:
doi:10.3969/j.issn.16716825.2012.01.019 △女,1967年8月生,本科,副主任医师,研究方向:肾病、风湿疾病
更新日期/Last Update: 1900-01-01