[1]仇朝红)△,魏玉海),李文渊).藏药智托洁白丸中没食子酸的定性与定量检测[J].郑州大学学报(医学版),2012,(01):88.
 QIU Chaohong,WEI Yuhai,LI Wenyuan.Qualitative and quantitative determination of gallic acid in tibetan Zhi Tuo Jie Bai Wan[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(01):88.
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藏药智托洁白丸中没食子酸的定性与定量检测
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年01期
页码:
88
栏目:
论著
出版日期:
2012-01-20

文章信息/Info

Title:
Qualitative and quantitative determination of gallic acid in tibetan Zhi Tuo Jie Bai Wan
作者:
仇朝红1)魏玉海1)李文渊2)
1)青海省食品药品检验所化学室 西宁 810016 2)青海大学医学院中藏药研究中心 西宁 810001
Author(s):
QIU Chaohong1)WEI Yuhai1)LI Wenyuan2)
1)Department of Chemistry,Qinghai Institute for Food and Drug Control,Xining 8100162)Research Center for Traditional Chinese Drug and Tibetan Drug,College of Medical Sciences,Qinghai University,Xining 810001
关键词:
智托洁白丸没食子酸薄层色谱法高效液相色谱法
Keywords:
Zhi Tuo Jie Bai Wangallic acidthin layer chromatographyhigh performance liquid chromatography
分类号:
R282.710.5
摘要:
目的:建立智托洁白丸处方中没食子酸定性与定量测定方法。方法:定性测定采用薄层色谱法。定量测定采用高效液相色谱法,色谱柱为SunfireODS C18柱(250 mm,4.6 mm),柱温30 ℃,以乙腈含体积分数0.1%三乙胺的体积分数0.1%磷酸溶液(体积比397) 为流动相;检测波长为273 nm,理论板数按没食子酸峰计算不低于3 000。结果:薄层色谱图中,供试品、对照药材和对照品在相应位置上显相同颜色的斑点,阴性对照无干扰。定量检测时,没食子酸进样量在0.015 7~0.283 0 μg范围内线性关系良好,标准曲线为Y=4×107X+6 532(R2=0.999 8);方法的精密度和重现性均良好(RSD分别为0.05%,1.43%),12 h内的稳定性良好(RSD=0.65%);平均回收率为98.07%,RSD=0.76%。9批样品中没食子酸平均含量为8.8 mg/g。结论:所建立的薄层色谱定性检测方法和高效液相色谱定量检测方法可用于智托洁白丸中没食子酸的定性鉴别和定量控制。
Abstract:
Aim:To establish the qualitative and quantitative determination methods of gallic acid in Zhi Tuo Jie Bai Wan.Methods:Thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) were established for the qualitative and quantitative determination of gallic acid.HPLC:SunfireODS C18 column(250 mm,4.6 mm),column temperature was 30 ℃,a mixture of 3 volumes of acetonitrile and 97 volumes of 0.1% phosphoric acid which contained 0.1% TEA was as the mobile phase,the detection wavelength was 273 nm,the number of theoretical plates of gallic acid peak should be no less than 3 000.Results:The spot in TLC of the test sample was similar in position and colour to those of the reference substance and control medicinal substances,and negative control had noninterference.For HPLC,the standard line was Y=4×107X+6 532(R2=0.999 8),the range was 0.015 7~0.283 0 μg,the precision and stability in 12 h were good (RSD=0.05%,0.65%),and the average recovery was 98.07%(RSD=0.76%).The average contents of gallic acid of 9 batches was 8.8 mg/g.Conclusion:TLC and HPLC method can be used for the qualitative determination and the quantitative control of gallic acid in Zhi Tuo Jie Bai Wan.

参考文献/References:

[1]国家药典委员会.中华人民共和国药典一部[S].2010年版.北京:中国医药科技出版社,2010:173 [2]刘起中,张可可.HPLC法测定五倍子中没食子酸的含量[J].中草药,2005,33(5):427 [3]韩双,席先蓉,黄平.贵州不同地区五倍子中没食子酸含量测定与品质评价[J].中国中医药信息杂志,2009,16(5):52 [4]龙海燕,彭飞城,姚蓉.高效液相色谱法测定复方青果颗粒中没食子酸含量 [J] .中南药学,2010,8(6):416 [5]潘国庆,冯学梅,马玉花.HPLC测定藏成药仲泽八味沉香散中没食子酸的含量 [J] .华西药学杂志,2006,21(4):388

备注/Memo

备注/Memo:
doi:10.3969/j.issn.16716825.2012.01.028 △男,1976年2月生,本科,主管药师,研究方向:药品检验方法,Email:qiuchaohong@126.com
更新日期/Last Update: 1900-01-01