[1]赵玉玲△,杨洪毅,马晓光,等.河南省耐多药结核分枝杆菌利福平耐药基因rpoB的突变特征*[J].郑州大学学报(医学版),2012,(02):166.
 ZHAO Yuling,YANG Hongyi,MA Xiaoguang,et al.Mutations of rpoB gene in multidrug resistant Mycobacterium tuberculosis clinical isolates from Henan province[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):166.
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河南省耐多药结核分枝杆菌利福平耐药基因rpoB的突变特征*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
166
栏目:
论著
出版日期:
2012-03-20

文章信息/Info

Title:
Mutations of rpoB gene in multidrug resistant Mycobacterium tuberculosis clinical isolates from Henan province
作者:
赵玉玲杨洪毅马晓光石洁李辉赵东阳
河南省疾病预防控制中心结核病预防控制所 郑州 450016
Author(s):
ZHAO Yuling YANG Hongyi MA Xiaoguang SHI Jie LI Hui ZHAO Dongyang
Institution of Tuberculosis Prevention and Control,Center for Disease Control and Prevention of Henan Province, Zhengzhou 450016
关键词:
结核分枝杆菌耐多药利福平rpoB突变
Keywords:
Mycobacterium tuberculosismultidrug resistantrifampinrpoBmutation
分类号:
R521
摘要:
目的:分析河南省耐多药(MDR)结核分枝杆菌利福平耐药相关基因rpoB的突变特征。方法:收集150株MDR结核分枝杆菌,应用PCR扩增利福平耐药相关基因rpoB编码区全长并测序,以结核分枝杆菌H37Rv标准株DNA序列为参比序列,分析菌株的突变特征。结果:MDR菌株rpoB基因编码区存在突变者占97.2%(140/144),突变在利福平耐药决定区(RRDR)内者占96.5%(139/144),RRDR内和RRDR外同时存在突变者占99.3%(139/140);突变热点依次为rpoB531、rpoB526和rpoB516。发现未被记录的15个突变位点和4个突变类型,其中rpoB1284位点突变可能是多态性位点,与利福平耐药无关。结论:检测河南地区结核分枝杆菌rpoB基因RRDR内位点的突变可预测MDR结核分枝杆菌的耐药性。
Abstract:
Aim:To analyze the characteristics of the mutations of rpoB genes in multidrug resistant Mycobacterium tuberculosis (MDRTB) clinical isolates from Henan province.Methods:A total of 150 MDRTB clinical strains were collected and the mutation patterns of rpoB full ORF was detected by PCR and sequencing with H37Rv as control.Results:97.2%(140/144) isolates carried mutations in the full ORF of rpoB gene,and 96.5%(139/144) isolates carried mutations in RRDR.99%(139/140) isolates showed mutations both in the RRDR and outside the RRDR.The top 3 loci with high mutation frequency were rpoB531, rpoB526 and rpoB516.Discovered 15 mutation loci and 4 mutation types that had not been recorded previously. Among them, rpoB1284 probably was polymorphism locus and had nothing to do with rifampcin resistance.Conclusion: It is of high sensitivity to discover MDRTB by detecting mutation loci within RRDR.

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备注/Memo

备注/Memo:
*河南省医学科技攻关基金资助项目201003140 女,1968年6月生,硕士,副主任技师,研究方向:微生物与分子生物学,Email:wbb558@hotmail.com
更新日期/Last Update: 1900-01-01