[1]单中杰),郭亮),侯箐岚),等.白藜芦醇对人肾细胞癌7860细胞周期、凋亡及PDCD5 mRNA表达的影响*[J].郑州大学学报(医学版),2012,(02):182.
 SHAN Zhongjie),GUO Liang),HOU Qinglan),et al.Effects of reservatrol on cell cycle, apoptosis and expression of PDCD5 mRNA in 7860 cells[J].JOURNAL OF ZHENGZHOU UNIVERSITY(MEDICAL SCIENCES),2012,(02):182.
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白藜芦醇对人肾细胞癌7860细胞周期、凋亡及PDCD5 mRNA表达的影响*
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《郑州大学学报(医学版)》[ISSN:1671-6825/CN:41-1340/R]

卷:
期数:
2012年02期
页码:
182
栏目:
论著
出版日期:
2012-03-20

文章信息/Info

Title:
Effects of reservatrol on cell cycle, apoptosis and expression of PDCD5 mRNA in 7860 cells
作者:
单中杰12)郭亮2)侯箐岚2)张楠2)魏金星1)#
1)郑州大学第一附属医院泌尿外科 郑州 450052 2)郑州人民医院泌尿外科 郑州 450012
Author(s):
SHAN Zhongjie12)GUO Liang2)HOU Qinglan2)ZHANG Nan2)WEI Jinxing1)
1)Department of Urology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052 2)Department of Urology,the People’s Hospital of Zhengzhou,Zhengzhou 450012
关键词:
肾细胞癌白藜芦醇PDCD57860细胞周期凋亡
Keywords:
renal cell carcinomareservatrolPDCD57860 cellcell cycleapoptosis
分类号:
R737.11
摘要:
目的:探讨白藜芦醇(RES)对人肾细胞癌7860细胞周期、凋亡及PDCD5 mRNA表达的影响。方法:取对数生长期的7860细胞,用含25 μmol/L RES的培养基(实验组)培养24、48及72 h后,应用流式细胞术检测7860细胞周期和凋亡情况,采用原位杂交技术检测细胞中PDCD5 mRNA的表达情况,均以不含RES的培养基作为空白对照。结果:25 μmol/L RES作用24 h后,实验组7860细胞G1期及G2期比例显著下降,S期比例明显升高。经25 μmol/L RES处理24、48及72 h后,实验组7860细胞凋亡细胞数(F组间=869.853,P<0.001)和PDCD5 mRNA的表达均高于空白对照组(F时间=10.067,P<0.001;F组间=649.629,P<0.001),差异有统计学意义。结论:RES可上调人肾细胞癌7860细胞PDCD5 mRNA的表达,使7860细胞阻滞在S期而诱导细胞凋亡。
Abstract:
Aim:To investigate the effects of reservatrol(RES) on the cell cycle, apoptosis and expression of PDCD5 mRNA in renal cell carcinoma 7860 cells.Methods:7860 cells in the experimental group were cultured with the medium containing 25 μmol/L RES for 24, 48 and 72 h,and the cells in the blank control group were cultured with the medium without RES.The cell cycle phase distribution and the cell apoptosis of 7860 cells were analyzed by using flow cytometry; and the expression of PDCD5 mRNA was analyzed by using in situ hybridization.Results:7860 cells in the experimental group at G1 phase and G2 phase ratio decreased significantly,and the proportion of S phase significantly increased after RES treatment for 24 h. The mRNA level of PDCD5 expression and the number of apoptotic cells in the experimental group were higher than the blank control group at 24,48 and 72 h.Conclusion:RES could upregulate the expression of PDCD5 mRNA in 7860 cells,which induces cell apoptosis by arresting cells at S phase.

参考文献/References:

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备注/Memo

备注/Memo:
*郑州市科委科技发展计划基金资助项目2009年1620 #通讯作者,男,1956年6月生,本科,教授,研究方向:泌尿系肿瘤, Email:weijinxing1956@163.com
更新日期/Last Update: 1900-01-01